Southeast University (China) View Institution's Website 13 articles published in JoVE Behavior High-Throughput Small Molecule Drug Screening For Age-Related Sleep Disorders Using Drosophila melanogaster Zheng Zhang1, Yuedong Wang1, Jiaying Zhao1, Shifeng Han1, Zi Chao Zhang1, Yao Tian1 1School of Life Science and Technology, Key Laboratory of Developmental Genes and Human Disease, Southeast University Presented is a protocol for high-throughput drug screening to improve sleep by monitoring the sleep behavior of fruit flies in an elderly Drosophila model. Bioengineering An Integrated Micro-Device System for Coral Growth and Monitoring Jinglun Zhao1, Tao Yuan2, Hui Huang2, Xiaolin Lu1 1State Key Laboratory of Digital Medical Engineering, School of Biological Science & Medical Engineering, Southeast University, 2Key Laboratory of Tropical Marine Bio-resources and Ecology, Guangdong Provincial Key Laboratory of Applied Marine Biology, South China Sea Institute of Oceanology, University of Chinese Academy of Sciences This protocol describes the development of a modular controllable micro-device system that can be applied for the long-term culturing and monitoring of sea corals. Cancer Research Generation of 3D Tumor Spheroids for Drug Evaluation Studies Wei Hou*1,2, Zilin Zhang*3,4, Jing Zhang3,4, Shihui Xu2,4, Menglan Li4, Xiaoran Li2,4, Zaozao Chen2,3, Cailian Wang1,2 1Department of Oncology, School of Medicine, Southeast University, 2State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, 3Institute of Medical Devices (Suzhou), Southeast University, 4Jiangsu Avatarget Biotechnology Co., Ltd. This article demonstrates a standardized method for constructing three-dimensional tumor spheroids. A strategy for spheroid observation and image-based deep-learning analysis using an automated imaging system is also described. Engineering Determination of Aggregate Surface Morphology at the Interfacial Transition Zone (ITZ) Kai Lyu1,2, Wei She1,2 1School of Materials Science and Engineering, Southeast University, 2State Key Laboratory of High Performance of Civil Engineering Materials Hereby, we proposed a protocol to illustrate the effect of aggregate surface morphology on the ITZ microstructure. The SEM-BSE image were quantitatively analyzed to obtain ITZ's porosity gradient via digital image processing and a K-means clustering algorithm was further employed to establish a relationship between porosity gradient and surface roughness. Neuroscience Cochlear Surface Preparation in the Adult Mouse Qiao-Jun Fang1,2, Fan Wu1, Renjie Chai2, Su-Hua Sha1 1Department of Pathology and Laboratory Medicine, Medical University of South Carolina, 2MOE Key Laboratory of Developmental Genes and Human Disease, Institute of Life Sciences, Southeast University This article presents a modified cochlear surface preparation method that requires decalcification and use of a cell and tissue adhesive to adhere the pieces of cochlear epithelia to 10 mm round cover slips for immunohistochemistry in adult mouse cochleae. Biochemistry Interfacial Molecular-level Structures of Polymers and Biomacromolecules Revealed via Sum Frequency Generation Vibrational Spectroscopy Xu Li1, Liang Ma1, Xiaolin Lu1 1State Key Laboratory of Bioelectronics, School of Biological Science & Medical Engineering, Southeast University Being comprehensively utilized, sum frequency generation (SFG) vibrational spectroscopy can help to reveal chain conformational order and secondary structural change happening at polymer and biomacromolecule interfaces. Medicine Polyethyleneimine-coated Iron Oxide Nanoparticles as a Vehicle for the Delivery of Small Interfering RNA to Macrophages In Vitro and In Vivo Na Jia*1, Haoan Wu*2, Juanli Duan1, Chunxue Wei1, Kaixuan Wang2, Yu Zhang 2, Xiaohua Mao1 1Key Laboratory of Ministry of Education for Developmental Genes & Human Diseases, Institute of Life Sciences and School of Medicine, Southeast University, 2State Key Laboratory of Bioelectronics, Jiangsu Key Laboratory for Biomaterials & Devices, School of Biological Science & Medical Engineering, Southeast University We describe a method of using polyethyleneimine (PEI)-coated superparamagnetic iron oxide nanoparticles for transfecting macrophages with siRNA. These nanoparticles can efficiently deliver siRNA to macrophages in vitro and in vivo and silence target gene expression. Neuroscience Microstate and Omega Complexity Analyses of the Resting-state Electroencephalography Fei Gao1, Huibin Jia2, Yi Feng1 1 This article describes the protocol underlying electroencephalography (EEG) microstate analysis and omega complexity analysis, which are two reference-free EEG measures and highly valuable to explore the neural mechanisms of brain disorders. Chemistry A Convenient Method for Extraction and Analysis with High-Pressure Liquid Chromatography of Catecholamine Neurotransmitters and Their Metabolites Li Xie1, Liqin Chen3, Pan Gu4, Lanlan Wei1, Xuejun Kang2 1School of Public Health of Southeast University, Laboratory of Environment and Biosafety Research Institute of Southeast University in Suzhou, 2Key Laboratory of Child Development and Learning Science (Ministry of Education), School of Biological Science & Medical Engineering, Southeast University, 3School of Public Health, Tianjin Medical University, 4British Columbia Academy, Nanjing Foreign Language School We present a convenient solid-phase extraction coupled to high-pressure liquid chromatography (HPLC) with electrochemical detection (ECD) for simultaneous determination of three monoamine neurotransmitters and two of their metabolites in infants' urine. We also identify the metabolite MHPG as a potential biomarker for the early diagnosis of brain damage for infants. Engineering Detecting the Water-soluble Chloride Distribution of Cement Paste in a High-precision Way Honglei Chang1,2, Song Mu2 1School of Materials Science and Engineering, Southeast University, 2State Key Laboratory of High Performance Civil Engineering Materials, Jiangsu Research Institute of Building Science A protocol for obtaining a water-soluble chloride profile by using a high precision milling method is presented. Biochemistry Personalized Peptide Arrays for Detection of HLA Alloantibodies in Organ Transplantation Pan Liu1, Tomokazu Souma1, Andrew Zu-Sern Wei1, Xueying Xie3, Xunrong Luo2, Jing Jin1 1Division of Nephrology and Hypertension, and the Center for Kidney Research and Therapeutics at the Feinberg Cardiovascular Research Institute, Northwestern University Feinberg School of Medicine, 2Surgery-Organ Transplantation, Northwestern University Feinberg School of Medicine, 3School of Biological Sciences and Medical Engineering, Southeast University Mismatches in human leukocyte antigen (HLA) sequences between organ donor and recipient pairs are the major cause of antibody-mediated rejection in organ transplantation. Here we present the use of custom antigen arrays that are based on individual donors' HLA sequences to probe anti-donor HLA alloantibodies in organ recipients. Neuroscience The Culture of Primary Motor and Sensory Neurons in Defined Media on Electrospun Poly-L-lactide Nanofiber Scaffolds Michelle K. Leach1, Zhang-Qi Feng2, Caitlyn C. Gertz3, Samuel J. Tuck3, Tara M. Regan3, Youssef Naim3, Andrea M. Vincent3, Joseph M. Corey1,3,4 1Department of Biomedical Engineering, University of Michigan, 2State Key Laboratory of Bioelectronics, Southeast University, 3Department of Neurology, University of Michigan, 4Geriatric Research, Education and Clinical Center, Veterans Affairs Ann Arbor Health System Aligned electrospun fibers direct the growth of neurons in vitro and are a potential component of nerve regeneration scaffolds. We describe a procedure for preparing electrospun fiber substrates and the serum-free culture of primary rat E15 sensory (DRG) and motor neurons. Visualization of neurons by immunocytochemistry is also included. Bioengineering Electrospinning Fundamentals: Optimizing Solution and Apparatus Parameters Michelle K. Leach1, Zhang-Qi Feng1,2, Samuel J. Tuck3, Joseph M. Corey1,3,4 1Department of Biomedical Engineering, University of Michigan, 2State Key Laboratory of Bioelectronics, Southeast University, 3Department of Neurology, University of Michigan, 4Geriatrics Research, Education and Clinical Center, Veterans Affairs Ann Arbor Healthcare Center Electrospinning techniques can create a variety of nanofibrous scaffolds for tissue engineering or other applications. We describe here a procedure to optimize the parameters of the electrospinning solution and apparatus to obtain fibers with the desired morphology and alignment. Common problems and troubleshooting techniques are also presented.