Nihon University 4 articles published in JoVE Biochemistry Quantitative Microtubule Fractionation Technique to Separate Stable Microtubules, Labile Microtubules, and Free Tubulin in Mouse Tissues Ayaka Hagita-Tatsumoto1,2, Tomohiro Miyasaka1,2,3 1Department of Neuropathology, Faculty of Life and Medical Sciences, Doshisha University, 2Center for Research in Neurodegenerative Diseases, Doshisha University, 3Laboratory of Physiology and Anatomy, School of Pharmacy, Nihon University Microtubules, which are tubulin polymers, play a crucial role as a cytoskeleton component in eukaryotic cells and are known for their dynamic instability. This study developed a method for fractionating microtubules to separate them into stable microtubules, labile microtubules, and free tubulin to evaluate the stability of microtubules in various mouse tissues. Medicine Novel Methods for Intranasal Administration Under Inhalation Anesthesia to Evaluate Nose-to-Brain Drug Delivery Takanori Kanazawa*1, Mitsuyoshi Fukuda*1, Naoto Suzuki1, Toyofumi Suzuki1 1Laboratory of Pharmaceutics, School of Pharmacy, Nihon University Here, we describe two novel methods of stable intranasal administration under inhalation anesthesia with minimal physical stress for experimental animals. We also describe a method for quantitative evaluation of drug distribution levels in the brain via the nose-to-brain pathway using radiolabeled [14C]-inulin as a model substrate of water-soluble macromolecules. Developmental Biology Imaging of Cell Shape Alteration and Cell Movement in Drosophila Gastrulation Using DE-cadherin Reporter Transgenic Flies M. Rezaul Karim1, Tomohiro Haruta2, Taro Matsumoto3, Hiroki Oda2, Hiroaki Taniguchi3,4,5 1Biotechnology and Genetic Engineering Department, Jahangirnagar University, 2JT Biohistory Research Hall, 3Division of Cell Regeneration and Transplantation, School of Medicine, Nihon University, 4Graduate School of Life and Medical Sciences, Doshisha University, 5Institute of Genetics and Animal Breeding of the Polish Academy of Sciences Herein we describe a procedure to capture live images of Drosophila gastrulation. This has enabled us to better understand the apical constriction involved in early development and further analyze mechanisms governing cellular movements during tissue structure modification. Developmental Biology An Efficient Method to Obtain Dedifferentiated Fat Cells Hiroaki Taniguchi*1, Tomohiko Kazama*1, Kazuhiro Hagikura*1, Chii Yamamoto1, Minako Kazama1, Yuki Nagaoka1, Taro Matsumoto1 1Division of Cell Regeneration and Transplantation, School of Medicine, Nihon University We have modified the conditions for DFAT cell generation and provide herein information regarding the use of an improved growth medium for the production of these cells.