Istituto di Fotonica e Nanotecnologie 1 article published in JoVE Medicine Non-Invasive Monitoring of Microvascular Oxygenation and Reactive Hyperemia using Hybrid, Near-Infrared Diffuse Optical Spectroscopy for Critical Care M. Atif Yaqub*1, Marta Zanoletti*1, Lorenzo Cortese1, Daniel Senciales Sánchez1, Caterina Amendola2, Lorenzo Frabasile2, Umut Karadeniz1, Jacqueline Martinez Garcia1, Marta Martin1, Jordi Cortes-Picas1, Alba Caballer3, Edgar Cortes3, Sara Nogales3, Alberto Tosi4, Talyta Carteano5, Diego Sanoja Garcia5, Jakub Tomanik6, Tessa Wagenaar6, Hsiao Mui6, Claudia Nunzia Guadagno7, Shahrzad Parsa8, Sanathana Konugolu Venkata Sekar7, Luc Demarteau6, Tijl Houtbeckers6, Udo M. Weigel8, Michele Lacerenza9, Mauro Buttafava9, Alessandro Torricelli2,10, Davide Contini2, Jaume Mesquida3, Turgut Durduran1,11 1ICFO - Institut de Ciències Fotòniques, The Barcelona Institute of Science and Technology, 2Dipartimento di Fisica, Politecnico di Milano, 3Critical Care Department, Parc Taulí Hospital Universitari, 4Dipartimento di Elettronica, Informazione e Bioingegneria, Politecnico di Milano, 5ASPHALION s.l., 6Splendo, 7BioPixS-Biophotonics Standards, IPIC, Tyndall National Institute, 8Hemophotonics s.l., 9PIONIRS s.r.l., 10Consiglio Nazionale delle Ricerche, Istituto di Fotonica e Nanotecnologie, 11Institució Catalana de Recerca i Estudis Avançats (ICREA) We describe a protocol to non-invasively and continuously measure absolute microvascular blood flow index and blood oxygen saturation using a multi-modal device based on near-infrared diffuse optics. We then evaluate the metabolic rate of oxygen consumption and reactive hyperemia utilizing a vascular occlusion test.
