Chapter 6
Principles of Mass Spectrometry
![Mass Spectrometry: Overview](https://cloudfront.jove.com/files/media/science-education/science-education-thumbs/12296.jpg)
Mass spectrometry is an analytical technique used to determine the molecular mass and molecular formula of a compound. The basic principle of mass…
![Mass Spectrometry: Isotope Effect](https://cloudfront.jove.com/files/media/science-education/science-education-thumbs/13040.jpg)
Most elements exist in nature as a mixture of isotopes. The isotopes differ in weight due to their respective number of neutrons. The molecular…
![Mass Spectrometers](https://cloudfront.jove.com/files/media/science-education/science-education-thumbs/13038.jpg)
This lesson details the instrumentation of a mass spectrometer—a physical instrument to perform mass spectrometry on analyte molecules and…
![Mass Spectrum: Overview](https://cloudfront.jove.com/files/media/science-education/science-education-thumbs/12297.jpg)
A mass spectrum is the graphical representation of the relative abundance of the charged fragments in an analyte plotted against their mass-to-charge…
![Mass Analyzers: Overview](https://cloudfront.jove.com/files/media/science-education/science-education-thumbs/14590.jpg)
The mass analyzer is a crucial component of the mass spectrometer. In the ionization chamber, the vaporized sample is bombarded with a high-energy…
![Mass Analyzers: Common Types](https://cloudfront.jove.com/files/media/science-education/science-education-thumbs/14591.jpg)
The quadrupole mass analyzer consists of four cylindrical metal rods arranged in a diamond carrying a DC voltage and a radio-frequency AC voltage.…
![Mass Spectrometry: Complex Analysis](https://cloudfront.jove.com/files/media/science-education/science-education-thumbs/14592.jpg)
Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex…
![Tandem Mass Spectrometry](https://cloudfront.jove.com/files/media/science-education/science-education-thumbs/14593.jpg)
Tandem mass spectrometry is a technique that uses multiple mass analyzers in series to obtain a higher selectivity and signal-to-noise ratio for the…
![A Mass Spectrometry-Based Approach to Identify Phosphoprotein Phosphatases and their Interactors](https://cloudfront.jove.com/files/thumbs/63805_t.png)
Most cellular processes are regulated by dynamic protein phosphorylation. More than three-quarters of proteins are phosphorylated, and phosphoprotein…
![Mass Spectrometry Analysis to Identify Ubiquitylation of EYFP-tagged CENP-A (EYFP-CENP-A)](https://cloudfront.jove.com/files/thumbs/61138_t.png)
Studying the structure and the dynamics of kinetochores and centromeres is important in understanding chromosomal instability (CIN) and cancer…