Fujian Maternity and Child Health Hospital 2 articles published in JoVE Biology Epon Post Embedding Correlative Light and Electron Microscopy Shuyuan Wang*1, Haiyan Xiong*1,2, Qiyuan Chang*1,3, Xudong Zhuang*4,5,6, Yaochen Wu1,3, Xinrui Wang4,5,6, Congxian Wu1, Zhifei Fu1,7,8 1Public Technology Service Center, Fujian Medical University, 2The School of Pharmacy, Fujian Medical University, 3The School of Basic Medical Sciences, Fujian Medical University, 4College of Clinical Medicine for Obstetrics & Gynecology and Pediatrics, Fujian Medical University, 5Medical Research Center, Fujian Maternity and Child Health Hospital, 6NHC Key Laboratory of Technical Evaluation of Fertility Regulation for Non-human Primate, Fujian Maternity and Child Health Hospital, 7Institute of Neuroscience, Fujian Medical University, 8Fujian Key Laboratory of Molecular Neurology, Fujian Medical University We present a detailed protocol for Epon post-embedding correlative light and electron microscopy using a fluorescent protein called mScarlet. This method can maintain the fluorescence and the ultrastructure simultaneously. This technique is amenable to a wide variety of biological applications. Biology Generation of Centromere-Associated Protein-E CENP-E-/- Knockout Cell Lines using the CRISPR/Cas9 System Meng-Fei Xu1,2, Jie Chen1,2, Yue Xu1,2, Jing-Lian Zhang1,2, Yi Zhou1,2, Jie-Jie He1,2, Shan Wu1,2, Ya-Lan Wei3,4, Zhen-Yu She1,2 1Department of Cell Biology and Genetics, The School of Basic Medical Sciences, Fujian Medical University, 2Key Laboratory of Stem Cell Engineering and Regenerative Medicine, Fujian Province University, 3College of Clinical Medicine for Obstetrics & Gynecology and Pediatrics, Fujian Medical University, 4Medical Research Center, Fujian Maternity and Child Health Hospital This article reports the construction of centromere-associated protein-E (CENP-E) knockout cells using the CRISPR/Cas9 system and three phenotype-based screening strategies. We have utilized the CENP-E knockout cell line to establish a novel approach to validate the specificity and toxicity of the CENP-E inhibitors, which is useful for drug development and biological research.
