Beijing Hospital of Traditional Chinese Medicine affiliated with Capital Medical University 1 article published in JoVE Medicine Application of Acupotomy in a Knee Osteoarthritis Model in Rabbit Xing LongFei1, Guo Yan2, Chen XiLin1, Hu TingYao1, Zhu WenTing3, Ma WeiWei1, Du Mei1, Xu Yue1, Guo ChangQing1 1School of Acupuncture-Moxibustion and Tuina, Beijing University of Chinese Medicine, 2Acupuncture and Moxibustion Department, Beijing Hospital of Traditional Chinese Medicine affiliated with Capital Medical University, 3The Third Affiliated Hospital of Beijing University of Chinese Medicine In this protocol, a knee osteoarthritis model was prepared using the modified Videman method, and the operation procedures and precautions of acupotomy are detailed. The effectiveness of acupotomy has been demonstrated by testing the mechanical properties of quadriceps femoris and tendon and the mechanical and morphological properties of cartilage.