Medical Experimental Center of Lanzhou University 1 article published in JoVE Medicine Subclavian Vein Blood Sampling in Conscious Rats Xiao-hua Zhang*1,2,3, Shun Peng*4, Zi-xu Pei5, Jing Sun6, Zhi-ping Wang1,2,3 1Institute of Urology, Lanzhou University Second Hospital, 2Key Laboratory of Gansu Province for Urological Diseases, 3Gansu Nephro-Urological Clinical Center, 4The First Clinical Medical College of Lanzhou University, 5The Second Clinical Medical College of Lanzhou University, 6Medical Experimental Center of Lanzhou University Here we present a combination of effective rat restriction and subclavian vein puncture methods that enable rapid, safe, and repeated blood collection in rats without anesthesia.