University of Tehran 1 article published in JoVE Immunology and Infection Detection of SARS-CoV-2 Receptor-Binding Domain Antibody using a HiBiT-Based Bioreporter Reza Rezaei1,2, Abera Surendran1,2, Ragunath Singaravelu1,2, Taylor R. Jamieson1,2, Parisa Taklifi3, Joanna Poutou1,2, Taha Azad1,2, Carolina S. Ilkow1,2 1Ottawa Hospital Research Institute, 2Department of Biochemistry, Microbiology and Immunology, University of Ottawa, 3Department of Biotechnology, University of Tehran The outlined protocol describes the procedure for producing the HiBiT-receptor-binding domain protein complex and its application for fast and sensitive detection of SARS-CoV-2 antibodies.