Korea Institute of Science and Technology (KIST) View Institution's Website 11 articles published in JoVE Biochemistry Malachite Green Assay for the Discovery of Heat-Shock Protein 90 Inhibitors Sayan Dutta Gupta1, Dae-Geun Song1, SueJung Lee1, Jae Wook Lee1, Jin-Soo Park1, Chrisostomos Prodromou2, Cheol-Ho Pan1 1Gangneung Institute of Natural Products, Korea Institute of Science and Technology (KIST), 2School of Life Sciences, University of Sussex The malachite green assay protocol is a simple and cost-effective method to discover heat shock protein 90 (Hsp90) suppressors, as well as other inhibitor compounds against ATP-dependent enzymes. Biochemistry Paper-Based Preconcentration and Isolation of Microvesicles and Exosomes Dohwan Lee*1, Kyung Wook Wee*1, Hyerin Kim*1, Sung Il Han1, Seungmin Kwak1,2, Dae Sung Yoon3, Jeong Hoon Lee1 1Department of Electrical Engineering, Kwangwoon University, 2Korea Institute of Science and Technology (KIST), 3School of Biomedical Engineering, Korea University Presented is a protocol to fabricate a paper-based device for the effective enrichment and isolation of microvesicles and exosomes. Medicine Measuring the Effects of Bacteria and Chemicals on the Intestinal Permeability of Caenorhabditis elegans Tram Anh Ngoc Le1, Baskar Selvaraj1,2, Jae Wook Lee1,2, Kyungsu Kang1,2 1Gangneung Institute of Natural Products, Korea Institute of Science and Technology, 2Division of Bio-Medical Science & Technology, Korea University of Science and Technology (UST) This protocol describes how to measure intestinal permeability of Caenorhabditis elegans. This method is helpful for basic biological research on intestinal health related to the interaction between intestinal bacteria and their host and for screening to identify probiotic and chemical agents to cure leaky gut syndrome and inflammatory bowel diseases. Bioengineering Synthesis of Multi-walled Carbon Nanotubes Modified with Silver Nanoparticles and Evaluation of Their Antibacterial Activities and Cytotoxic Properties Youngmin Seo*1, Chanhwi Park*2, Jaewoo Son2, Kyungwoo Lee2, Jangsun Hwang2, Yeonho Jo2, Dohyun Lee2, Muhammad Saad Khan2, Sachin Ganpat Chavan2, Yonghyun Choi2, Dasom Kim2, Assaf A Gilad3, Jonghoon Choi2 1Center for Biomaterials, Biomedical Research Institute, Korea Institute of Science and Technology, 2School of Integrative Engineering, Chung-Ang University, 3Division of Synthetic Biology and Regenerative Medicine, Institute for Quantitative Health Science and Engineering, Michigan State University In this study, antimicrobial nanomaterials were synthesized by acidic oxidation of multiwalled carbon nanotubes and subsequent reductive deposition of silver nanoparticles. Antimicrobial activity and cytotoxicity tests were performed with the as-prepared nanomaterials. Neuroscience Implantation of Chronic Silicon Probes and Recording of Hippocampal Place Cells in an Enriched Treadmill Apparatus Anvar Sariev1,2, Jinho Chung1, Dajung Jung1,3, Farnaz Sharif1,2, Ju-Young Lee1,2, Soyoun Kim1, Sebastien Royer1,2 1Center for Functional Connectomics, Korea Institute of Science and Technology, 2Biomedical department, KIST school, University of Science and Technology, 3Department of Biological Sciences, Korea Advanced Institute of Science and Technology We describe the diverse steps to implant chronic silicon probes and to record place cells in mice that are running head-fixed on a cue-enriched treadmill apparatus. Medicine Measuring the Effect of Chemicals on the Growth and Reproduction of Caenorhabditis elegans So Young Lee1, Kyungsu Kang1 1Systems Biotechnology Research Center, Korea Institute of Science and Technology A basic protocol to evaluate the toxicity of chemicals in a model animal, Caenorhabditis elegans, is described. The method is convenient and useful for the development of pharmaceuticals as well as for the risk assessment of various environmental pollutants. Bioengineering Merging Ion Concentration Polarization between Juxtaposed Ion Exchange Membranes to Block the Propagation of the Polarization Zone Minyoung Kim1,2, Hyunjoon Rhee1,3, Ji Yoon Kang1, Tae Song Kim1, Rhokyun Kwak1 1Center for BioMicrosystems, Korea Institute of Science and Technology, 2Department of Mechanical Engineering, Seoul National University, 3Department of Industrial Engineering, University of Illinois Urbana-Champaign The protocol for a novel ion concentration polarization (ICP) platform that can stop the propagation of the ICP zone, regardless of the operating conditions is described. This unique ability of the platform lies in the use of merging ion depletion and enrichment, which are two polarities of the ICP phenomenon. Medicine Combined Near-infrared Fluorescent Imaging and Micro-computed Tomography for Directly Visualizing Cerebral Thromboemboli Dong-Eog Kim1, Jeong-Yeon Kim1, Su-Kyoung Lee1, Ju Hee Ryu2, Ick Chan kwon2, Cheol-Hee Ahn3, Kwangmeyung Kim2, Dawid Schellingerhout4 1Molecular Imaging and Neurovascular Research Laboratory, Dongguk University College of Medicine, 2Biomedical Research Center, Korea Institute of Science and Technology, 3Research Institute of Advanced Materials, Department of Materials Science and Engineering, Seoul National University, 4Departments of Radiology and Cancer Systems Imaging, University of Texas M.D. Anderson Cancer Center This protocol describes the application of combined near-infrared fluorescent (NIRF) imaging and micro-computed tomography (microCT) for visualizing cerebral thromboemboli. This technique allows the quantification of thrombus burden and evolution. The NIRF imaging technique visualizes fluorescently labeled thrombus in excised brain, while the microCT technique visualizes thrombus inside living animals using gold-nanoparticles. Neuroscience Intracerebroventricular Injection of Amyloid-β Peptides in Normal Mice to Acutely Induce Alzheimer-like Cognitive Deficits Hye Yun Kim1,2, Dongkeun K. Lee1, Bo-Ryehn Chung1,3, Hyunjin V. Kim1,4, YoungSoo Kim1,4 1Center for Neuro-Medicine, Korea Institute of Science and Technology, 2Research Institute, GoshenBiotech, Inc., 3Department of Chemical and Biological Engineering, Princeton University, 4Biological Chemistry Program, Korea University of Science and Technology The amyloid-β (Aβ)-injected animal model enables the administration of a defined quantity and species of Aβ fragments and reduces individual differences within each study group. This protocol describes the intracerebroventricular (ICV) injection of Aβ without stereotactic instruments, enabling the production of Alzheimer-like behavioral abnormalities in normal mice. Neuroscience Imaging Membrane Potential with Two Types of Genetically Encoded Fluorescent Voltage Sensors Sungmoo Lee1,2, Hong Hua Piao2, Masoud Sepheri-Rad2, Arong Jung2,3, Uhna Sung2, Yoon-Kyu Song1,4, Bradley J. Baker2 1Department of Transdisciplinary Studies, Graduate School of Convergence Science and Technology, Seoul National University, 2Center for Functional Connectomics, Korea Institute of Science and Technology, 3College of Life Sciences and Biotechnology, Korea University, 4Advanced Institutes of Convergence Technology A method for imaging changes in membrane potential using genetically encoded voltage indicators is described. Neuroscience High-density EEG Recordings of the Freely Moving Mice using Polyimide-based Microelectrode Mina Lee1,2, Dongwook Kim1,2, Hee-Sup Shin1,2, Ho-Geun Sung3, Jee Hyun Choi1,2 1Center for Neural Science , Korea Institute of Science and Technology (KIST), 2Department of Neuroscience, University of Science and Technology, 3Fab Service Department, Korea Advanced Nano Fab Center In this article, we described the surgery procedure and handling tips for implantation of ultra-thin polyimide-based microelectrode array (PBM-array) on the mouse skull for acquisition of high-density encephalography (EEG) in a mouse model.