Radboud University Nijmegen Medical Centre View Institution's Website 9 articles published in JoVE Cancer Research Management of Respiratory Motion Artefacts in 18F-fluorodeoxyglucose Positron Emission Tomography using an Amplitude-Based Optimal Respiratory Gating Algorithm Willem Grootjans1, Peter Kok2, Jurrian Butter2, Erik Aarntzen2 1Department of Radiology, Leiden University Medical Centre, 2Department of Radiology and Nuclear Medicine, Radboud University Medical Centre Amplitude-based optimal respiratory gating (ORG) effectively removes respiratory-induced motion blurring from clinical 18F-fluorodeoxyglucose (FDG) positron emission tomography (PET) images. Correction of FDG-PET images for these respiratory motion artefacts improves image quality, diagnostic and quantitative accuracy. Removal of respiratory motion artefacts is important for adequate clinical management of patients using PET. Bioengineering Mimicking and Measuring Occlusal Erosive Tooth Wear with the "Rub&Roll" and Non-contact Profilometry Jan L. Ruben1, Gert-Jan Truin1, Bas A.C. Loomans1, Marie-Charlotte D.N.J.M. Huysmans1 1Department of Dentistry, Radboud University Medical Center The Rub&Roll can mimic the chewing cycle, allowing variation of chewing force, sliding distance, chewing velocity, number of cycles, and frequency, and with a combination of erosive and abrasive challenges can result in a complex simulation of oral ageing. Medicine A Quantitative Sensory Testing Paradigm to Obtain Measures of Pain Processing in Patients Undergoing Breast Cancer Surgery Noud van Helmond1, Hans Timmerman1, Søren S. Olesen2, Asbjørn M. Drewes2, Joris Kleinhans3, Oliver H. Wilder-Smith1, Kris C. Vissers1, Monique A. Steegers1 1Department of Anesthesiology, Pain, and Palliative Medicine, Radboud University Medical Center, 2Mech-Sense, Department of Gastroenterology & Hepatology, Aalborg University Hospital, 3Medical Technology & Clinical Physics, Radboud University Medical Center Persistent postsurgical pain may be related to changes in pain processing. By assessing pain in response to standardized stimuli, changes in pain processing can be elucidated. We present methods to obtain pain thresholds to different stimuli and a measure of endogenous analgesia in patients undergoing breast cancer surgery. Immunology and Infection Visualizing Intracellular SNARE Trafficking by Fluorescence Lifetime Imaging Microscopy Daniëlle R. J. Verboogen1, Maksim V. Baranov1, Martin ter Beest1, Geert van den Bogaart1 1Department of Tumor Immunology, Radboud University Medical Center This protocol describes a new method allowing for the quantitative visualization of complex formation of SNARE proteins, based on Förster resonance energy transfer, and fluorescence lifetime imaging microscopy. Neuroscience High-throughput Analysis of Locomotor Behavior in the Drosophila Island Assay Ilse Eidhof*1, Michaela Fenckova*1, Dei M. Elurbe2, Bart van de Warrenburg3, Anna Castells Nobau*1, Annette Schenck*1 1Department of Human Genetics, Donders Institute for Brain, Cognition and Behaviour, Radboud University Medical Center, 2Centre for Molecular and Biomolecular Informatics, Radboud Institute for Molecular Life Sciences, Radboud University Medical Center, 3Department of Neurology, Donders Institute for Brain, Cognition and Behaviour, Radboud University Medical Center The island assay is a relatively new, cost-effective assay that can be used to evaluate the basic locomotor behavior of Drosophila melanogaster. This manuscript describes algorithms for automatic data processing and objective quantification of island assay data, making this assay a sensitive, high-throughput readout for large genetic or pharmacological screens. Biology Cellular Redox Profiling Using High-content Microscopy Tom Sieprath1,2, Tobias Corne1,2, Joke Robijns1, Werner J. H. Koopman3, Winnok H. De Vos1,2 1Laboratory of Cell Biology and Histology, Department of Veterinary Sciences, University of Antwerp, 2Cell Systems and Imaging Research Group (CSI), Department of Molecular Biotechnology, Ghent University, 3Department of Biochemistry , Radboud Institute for Molecular Life Sciences, Radboud University Medical Center This paper presents a high-content microscopy workflow for simultaneous quantification of intracellular ROS levels, as well as mitochondrial membrane potential and morphology – jointly referred to as mitochondrial morphofunction – in living adherent cells using the cell-permeant fluorescent reporter molecules 5-(and-6)-chloromethyl-2',7'-dichlorodihydrofluorescein diacetate, acetyl ester (CM-H2DCFDA) and tetramethylrhodamine methylester (TMRM). Immunology and Infection Lymphocyte Isolation from Human Skin for Phenotypic Analysis and Ex Vivo Cell Culture Xuehui He1, Vivian L. de Oliveira1, Romy Keijsers2, Irma Joosten1, Hans JPN Koenen1 1Laboratory of Medical Immunology, Department of Laboratory Medicine, Radboud University Medical Centre, 2Department of Dermatology, Radboud University Medical Centre We describe a protocol to efficiently isolate skin resident T cells from human skin biopsies. This protocol yields sufficient numbers of viable human skin resident lymphocytes for flow cytometric analysis and ex vivo culture. Behavior Event-related Potentials During Target-response Tasks to Study Cognitive Processes of Upper Limb Use in Children with Unilateral Cerebral Palsy Ingar Marie Zielinski1, Bert Steenbergen1,2, C. Marjolein Baas1, Pauline Aarts3, Marijtje L. A. Jongsma1 1Behavioural Science Institute, Radboud University Nijmegen, 2School of Psychology, Australian Catholic University, 3Department of Pediatric Rehabilitation, Sint Maartenskliniek Several children with unilateral Cerebral Palsy seem to disregard the preserved capacity of their affected upper limb. This Developmental Disregard is extensively described in the literature but the involved cognitive processes have not been studied. To study underlying cognitive factors of upper limb control, an event-related potential protocol was developed. Immunology and Infection Using Bioluminescent Imaging to Investigate Synergism Between Streptococcus pneumoniae and Influenza A Virus in Infant Mice Kirsty R. Short1, Dimitri A. Diavatopoulos2, Patrick C. Reading1, Lorena E. Brown1, Kelly L. Rogers3, Richard A. Strugnell1, Odilia L.C. Wijburg1 1Department of Microbiology and Immunology, University of Melbourne, 2Laboratory of Pediatric Infectious Diseases, Radboud University Nijmegen Medical Centre, 3The Centre for Dynamic Imaging, The Walter and Eliza Hall Institute for Medical Research A concurrent infection with influenza A virus is one of the factors implicated in the induction of invasive pneumococcal disease during asymptomatic Streptococcus pneumoniae carriage. Here we describe a mixed infection method using infant mice to investigate the synergism between these two respiratory pathogens.