Brock University View Institution's Website 5 articles published in JoVE Bioengineering Proper Positioning and Restraint of a Rat Hind Limb for Focused High Resolution Imaging of Bone Micro-architecture Using In Vivo Micro-computed Tomography Amanda B. Longo1, Sandra M. Sacco1, Wendy E. Ward1,2 1Department of Kinesiology, Faculty of Applied Health Sciences, Brock University, 2Department of Health Sciences, Faculty of Applied Health Sciences, Brock University This paper instructs users of in vivo micro-computed tomography (µCT) scanners how to anesthetize, correctly position and restrain the hind limb of a rat for minimal movement during high-resolution imaging of the tibia. The result is high quality images that can be processed to accurately quantify bone micro-architecture. Behavior Exploring the Use of Isolated Expressions and Film Clips to Evaluate Emotion Recognition by People with Traumatic Brain Injury Barbra Zupan1, Dawn Neumann2 1Department of Applied Linguistics, Brock University, 2Department of Physical Medicine and Rehabilitation, Indiana School of Medicine and Rehabilitation Hospital of Indiana This paper describes how to implement a battery of behavioral tasks to examine emotion recognition of isolated facial and vocal emotion expressions, and a novel task using commercial television and film clips to assess multimodal emotion recognition that includes contextual cues. Medicine Enhancement of Apoptotic and Autophagic Induction by a Novel Synthetic C-1 Analogue of 7-deoxypancratistatin in Human Breast Adenocarcinoma and Neuroblastoma Cells with Tamoxifen Dennis Ma1, Jonathan Collins2, Tomas Hudlicky2, Siyaram Pandey1 1Department of Chemistry and Biochemistry, University of Windsor, 2Chemistry Department and Centre for Biotechnology, Brock University We have synthesized a novel analogue of pancratistatin with comparable anti-cancer activity as native pancratistatin; interestingly, combinatory treatment with tamoxifen yielded a drastic enhancement in apoptotic and autophagic induction by mitochondrial targeting with minimal effect on noncancerous fibroblasts. Thus, JCTH-4 in combination with tamoxifen could provide a safe anti-cancer therapy. Neuroscience Physiological Experimentation with the Crayfish Hindgut: A Student Laboratory Exercise Ann S. Cooper*1, Bonnie Leksrisawat*1, Allison B. Gilberts*1, A. Joffre Mercier*2, Robin L. Cooper*1 1Department of Biology, University of Kentucky, 2Department of Biological Sciences, Brock University In this report we demonstrate techniques that can be used to investigate the biology of the crayfish hindgut. We show how to dissect a crayfish abdomen and study the associated anatomy, physiology and modulation of activity. The peristaltic activity and strength of contractions are measured using a force transducer. Neuroscience Recording Behavioral Responses to Reflection in Crayfish A. Joffre Mercier1, Holly Y. May1 1Department of Biological Sciences, Brock University We have developed two methods for studying effects of visual cues on behavior in the absence of tactile and chemical cues. One method involves videotaping responses of crayfish to reflective walls in an aquarium; the other examines effects of visual inputs provided by a live crayfish behind a transparent partition.