Eureka Therapeutics 1 article published in JoVE Medicine Real-time Cytotoxicity Assays in Human Whole Blood Ching-Wen Hsiao*1, Yen-Ting Lo*1, Hong Liu2, Sonny C. Hsiao1 1Research and Development, Adheren, Inc, 2Research and Development, Eureka Therapeutics The whole blood cytotoxicity assay (WCA) is a cytotoxicity assay developed by incorporating high-throughput cell positioning technology with fluorescence microscopy and automated image processing. Here, we describe how lymphoma cells treated with an anti-CD20 antibody can be analyzed real-time in human whole blood to provide quantitative cellular cytotoxicity analysis.