JoVE Journal
Bioengineering
Bioengineering
A subscription to JoVE is required to view this content. Sign in or start your free trial.
Chapters
Summary
This protocol describes the method, materials, equipment and steps for bottom-up preparation of RNA and protein producing synthetic cells. The inner aqueous compartment of the synthetic cells contained the S30 bacterial lysate encapsulated within a lipid bilayer (i.e., stable liposomes), using a water-in-oil emulsion transfer method.