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The Journal of Visualized Experiments (JoVE) is a peer reviewed, PubMed-indexed video journal. Our mission is to increase the productivity of scientific research.

 
 
 
 
 
 

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Monitoring Functionality and Morphology of Vasculature<span style='display: none;'> Recruited by Factors Secreted by Fast-growing Tumor-generating Cells</span>…

Monitoring Functionality and Morphology of Vasculature Recruited by Factors Secreted by Fast-growing Tumor-generating Cells
Published 11/23/2014

We describe a matrigel plug assay to illustrate angiogenic potential of a pool of factors secreted by cancer cells using two complementary imaging modalities, ultrasound and endomicroscopy. The matrigel, an extracellular matrix (ECM)-mimic gel, is utilized to introduce the host (mouse) with angiogenic factors secreted to the conditioned media (C.M.). 

Universal Hand-held Three-dimensional Optoacoustic Imaging<span style='display: none;'> Probe for Deep Tissue Human Angiography and Functional Preclinical Studies in Real Time</span>…

Universal Hand-held Three-dimensional Optoacoustic Imaging Probe for Deep Tissue Human Angiography and Functional Preclinical Studies in Real Time
Published 11/04/2014

We provide herein a detailed description of the experimental protocol for imaging with a newly developed hand-held optoacoustic (photoacoustic) system for three-dimensional functional and molecular imaging in real time. The demonstrated powerful performance and versatility may define new application areas of the optoacoustic technology in preclinical research and clinical practice.

Fabricating Complex Culture Substrates Using Robotic<span style='display: none;'> Microcontact Printing (R-µCP) and Sequential Nucleophilic Substitution</span>…

Fabricating Complex Culture Substrates Using Robotic Microcontact Printing (R-µCP) and Sequential Nucleophilic Substitution
Published 10/31/2014

Cell culture substrates functionalized with microscale patterns of biological ligands have immense utility in the field of tissue engineering. Here, we demonstrate the versatile and automated manufacture of tissue culture substrates with multiple, micropatterned poly(ethylene glycol) brushes presenting orthogonal chemistries that enable spatially precise and site-specific immobilization of biological ligands.

Quantification and Size-profiling of Extracellular Vesicles <span style='display: none;'>Using Tunable Resistive Pulse Sensing</span>…

Quantification and Size-profiling of Extracellular Vesicles Using Tunable Resistive Pulse Sensing
Published 10/19/2014

Extracellular vesicles play important roles in physiological and pathological processes, including coagulation, immune responses, and cancer or as potential therapeutic agents in drug delivery or regenerative medicine. This protocol presents methods for the quantification and size characterization of isolated and non-isolated extracellular vesicles in various fluids using tunable resistive pulse sensing.

Multimodal Optical Microscopy Methods Reveal Polyp Tissue<span style='display: none;'> Morphology and Structure in Caribbean Reef Building Corals</span>…

Multimodal Optical Microscopy Methods Reveal Polyp Tissue Morphology and Structure in Caribbean Reef Building Corals
Published 9/05/2014

An integrated suite of imaging techniques has been applied to determine polyp morphology and tissue structure in the Caribbean corals Montastraeaannularis and M. faveolata. Fluorescence, serial block face, and two-photon confocal laser scanning microscopy have identified lobate structure, polyp walls, and estimated chromatophore and zooxanthellae densities and distributions.

Measuring Neural and Behavioral Activity During Ongoing<span style='display: none;'> Computerized Social Interactions: An Examination of Event-Related Brain Potentials</span>…

Measuring Neural and Behavioral Activity During Ongoing Computerized Social Interactions: An Examination of Event-Related Brain Potentials
Published 11/15/2014

Research on social exclusion has grown tremendously in recent years. As the field expands, it is imperative to develop sophisticated methodologies allowing for the simultaneous measurement of neural and behavioral outcomes during social exclusion. This protocol utilizes event-related brain potentials to record ongoing neural activity during computerized social interactions.

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