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Slow-release Drug Delivery through Elvax 40W to the Rat Retina: Implications for the Treatment of Chronic Conditions

*1, *1, 2,3, 1, 1,2, 2,3,4

1Biotechnology and Applied Clinical Sciences Department, University of L'Aquila, 2ARC Centre of Excellence in Vision Science, 3John Curtin School of Medical Research, Australian National University, 4ANU Medical School, Australian National University

* These authors contributed equally

 

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Most Recent Video Articles

Isolation of Human Monocytes by Double Gradient<span style='display: none;'> Centrifugation and Their Differentiation to Macrophages in Teflon-coated Cell Culture Bags</span>…

Isolation of Human Monocytes by Double Gradient Centrifugation and Their Differentiation to Macrophages in Teflon-coated Cell Culture Bags
Published 9/09/2014

We present a simple and efficient protocol for the generation of human macrophages. Buffy coats are processed by double density gradient centrifugation and isolated monocytes are then differentiated to macrophages in Teflon-coated cell culture bags. This maximizes macrophage yields and facilitates cell harvesting for subsequent experiments.

Heterotopic Auxiliary Rat Liver Transplantation With<span style='display: none;'> Flow-regulated Portal Vein Arterialization in Acute Hepatic Failure</span>…

Heterotopic Auxiliary Rat Liver Transplantation With Flow-regulated Portal Vein Arterialization in Acute Hepatic Failure
Published 9/13/2014

Auxiliary liver transplantation provides a temporary support in acute hepatic failure, until regeneration of the failing liver. The heterotopic auxiliary liver transplantation (HALT) with portal vein arterialization (PVA) renders sufficient liver function. We developed an analogous technique in the rat, to examine the influence of the portal vein arterialization on the morphology and function of the graft.

Integrating a Triplet-triplet Annihilation Up-conversion<span style='display: none;'> System to Enhance Dye-sensitized Solar Cell Response to Sub-bandgap Light</span>…

Integrating a Triplet-triplet Annihilation Up-conversion System to Enhance Dye-sensitized Solar Cell Response to Sub-bandgap Light
Published 9/12/2014

An integrated device, incorporating a dye-sensitized solar cell and triplet-triplet annihilation up-conversion unit was produced, affording enhanced light harvesting, from a wider section of the solar spectrum. Under modest irradiation levels a significantly enhanced response to low energy photons was demonstrated, yielding a record figure of merit for dye-sensitized solar cells.

Real-Time DC-dynamic Biasing Method for Switching Time<span style='display: none;'> Improvement in Severely Underdamped Fringing-field Electrostatic MEMS Actuators</span>…

Real-Time DC-dynamic Biasing Method for Switching Time Improvement in Severely Underdamped Fringing-field Electrostatic MEMS Actuators
Published 8/15/2014

The robust device design of fringing-field electrostatic MEMS actuators results in inherently low squeeze-film damping conditions and long settling times when performing switching operations using conventional step biasing. Real-time switching time improvement with DC-dynamic waveforms reduces the settling time of fringing-field MEMS actuators when transitioning between up-to-down and down-to-up states.

Synthesis and Characterization of Functionalized<span style='display: none;'> Metal-organic Frameworks</span>…

Synthesis and Characterization of Functionalized Metal-organic Frameworks
Published 9/05/2014

Synthesis, activation, and characterization of intentionally designed metal-organic framework materials is challenging, especially when building blocks are incompatible or unwanted polymorphs are thermodynamically favored over desired forms. We describe how applications of solvent-assisted linker exchange, powder X-ray diffraction in capillaries and activation via supercritical CO2 drying, can address some of these challenges.

Multimodal Optical Microscopy Methods Reveal Polyp Tissue<span style='display: none;'> Morphology and Structure in Caribbean Reef Building Corals</span>…

Multimodal Optical Microscopy Methods Reveal Polyp Tissue Morphology and Structure in Caribbean Reef Building Corals
Published 9/05/2014

An integrated suite of imaging techniques has been applied to determine polyp morphology and tissue structure in the Caribbean corals Montastraeaannularis and M. faveolata. Fluorescence, serial block face, and two-photon confocal laser scanning microscopy have identified lobate structure, polyp walls, and estimated chromatophore and zooxanthellae densities and distributions.

Optimized Negative Staining: a High-throughput Protocol for <span style='display: none;'>Examining Small and Asymmetric Protein Structure by Electron Microscopy</span>…

Optimized Negative Staining: a High-throughput Protocol for Examining Small and Asymmetric Protein Structure by Electron Microscopy
Published 8/15/2014

More than half of proteins are small proteins (molecular mass <200 kDa) that are challenging for both electron microscope imaging and three-dimensional reconstructions. Optimized negative staining is a robust and high-throughput protocol to obtain high contrast and relatively high resolution (~1 nm) images of small asymmetric proteins or complexes under different physiological conditions.

Comprehensive Analysis of Transcription Dynamics from Brain <span style='display: none;'>Samples Following Behavioral Experience</span>…

Comprehensive Analysis of Transcription Dynamics from Brain Samples Following Behavioral Experience
Published 8/26/2014

This manuscript describes a protocol that applies comprehensive profiling for analysis of transcriptional programs induced in specific brain nuclei of rodents following behavioral paradigms. Herein, this approach is illustrated in the context of profiling genes induced in the nucleus accumbens (NAc) of mice following acute cocaine exposure, utilizing microfluidic qPCR arrays.

Getting to Compliance in Forced Exercise in Rodents: A<span style='display: none;'> Critical Standard to Evaluate Exercise Impact in Aging-related Disorders and Disease</span>…

Getting to Compliance in Forced Exercise in Rodents: A Critical Standard to Evaluate Exercise Impact in Aging-related Disorders and Disease
Published 8/22/2014

Exercise may alleviate behavioral impairments associated with neurodegenerative diseases. Studies of the mechanisms underlying exercise-mediated effects have increased in disease models, but the neurobiological background accompanying these diseases, specifically aging, is likely not considered. Here, we illustrate measures to increase the likelihood of a successful exercise paradigm in aged rats.

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