Journal of Visualized Experiments

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Date ID Title
02/03/123887Spectral Karyotyping to Study Chromosome Abnormalities in Humans and Mice with Polycystic Kidney Disease
02/03/123644The CYP2D6 Animal Model: How to Induce Autoimmune Hepatitis in Mice
02/02/123490Preparation of Intact Bovine Tail Intervertebral Discs for Organ Culture
02/02/123455Simultaneous Synthesis of Single-walled Carbon Nanotubes and Graphene in a Magnetically-enhanced Arc Plasma
02/01/123678Investigating Intestinal Inflammation in DSS-induced Model of IBD
02/01/122434Brain Imaging Investigation of the Impairing Effect of Emotion on Cognition
01/31/123587A 3D System for Culturing Human Articular Chondrocytes in Synovial Fluid
01/31/123350Organotypic Slice Cultures of Embryonic Ventral Midbrain: A System to Study Dopaminergic Neuronal Development in vitro
01/30/123528Rodent Stereotaxic Surgery and Animal Welfare Outcome Improvements for Behavioral Neuroscience
01/30/123362Visualization of Caenorhabditis elegans Cuticular Structures Using the Lipophilic Vital Dye DiI
01/29/123638The Mouse Forced Swim Test
01/28/123769The Tail Suspension Test
01/27/123571Measuring Peptide Translocation into Large Unilamellar Vesicles
01/27/123410Monitoring Kinase and Phosphatase Activities Through the Cell Cycle by Ratiometric FRET
01/26/123583Metabolic Pathway Confirmation and Discovery Through 13C-labeling of Proteinogenic Amino Acids
01/26/123485Tissue Preparation and Immunostaining of Mouse Sensory Nerve Fibers Innervating Skin and Limb Bones
01/25/123636Polymer Microarrays for High Throughput Discovery of Biomaterials
01/25/123493Quantifying Mixing using Magnetic Resonance Imaging
01/24/123540Intracellular Refolding Assay
01/24/123496Femoral Arterial and Venous Catheterization for Blood Sampling, Drug Administration and Conscious Blood Pressure and Heart Rate Measurements
01/23/123502Pull-down of Calmodulin-binding Proteins
01/23/123454Quantitative Live Cell Fluorescence-microscopy Analysis of Fission Yeast
01/22/123551The Use of Thermal Infra-Red Imaging to Detect Delayed Onset Muscle Soreness
01/21/123491Analysis of Cell Cycle Position in Mammalian Cells
01/20/123535Real-time fMRI Biofeedback Targeting the Orbitofrontal Cortex for Contamination Anxiety
01/20/123387Utilizing Transcranial Magnetic Stimulation to Study the Human Neuromuscular System
01/19/123345Patch-clamp Capacitance Measurements and Ca2+ Imaging at Single Nerve Terminals in Retinal Slices
01/19/123330Analysis of Trunk Neural Crest Cell Migration using a Modified Zigmond Chamber Assay
01/18/123495Assaying the Kinase Activity of LRRK2 in vitro
01/18/123367Rotating Cell Culture Systems for Human Cell Culture: Human Trophoblast Cells as a Model
01/17/123562Modified Yeast-Two-Hybrid System to Identify Proteins Interacting with the Growth Factor Progranulin
01/17/123349Parallel-plate Flow Chamber and Continuous Flow Circuit to Evaluate Endothelial Progenitor Cells under Laminar Flow Shear Stress
01/16/123394A Simple Method for Imaging Arabidopsis Leaves Using Perfluorodecalin as an Infiltrative Imaging Medium
01/16/123370Orthogonal Protein Purification Facilitated by a Small Bispecific Affinity Tag
01/15/123570Gold Nanostar Synthesis with a Silver Seed Mediated Growth Method
01/14/123609Intravital Microscopy of the Spleen: Quantitative Analysis of Parasite Mobility and Blood Flow
01/13/123422Monitoring Cleaved Caspase-3 Activity and Apoptosis of Immortalized Oligodendroglial Cells using Live-cell Imaging and Cleaveable Fluorogenic-dye Substrates Following Potassium-induced Membrane Depolarization
01/13/123308Implantation of a Carotid Cuff for Triggering Shear-stress Induced Atherosclerosis in Mice
01/12/123464Presynaptic Dopamine Dynamics in Striatal Brain Slices with Fast-scan Cyclic Voltammetry
01/12/123180Cut-loading: A Useful Tool for Examining the Extent of Gap Junction Tracer Coupling Between Retinal Neurons
01/11/123628In vitro Assembly of Semi-artificial Molecular Machine and its Use for Detection of DNA Damage
01/11/123830Cultivation of Human Neural Progenitor Cells in a 3-dimensional Self-assembling Peptide Hydrogel
01/10/123655Locked Nucleic Acid Flow Cytometry-fluorescence in situ Hybridization (LNA flow-FISH): a Method for Bacterial Small RNA Detection
01/10/123510Immunostaining of Dissected Zebrafish Embryonic Heart
01/09/123383High-throughput Crystallization of Membrane Proteins Using the Lipidic Bicelle Method
01/09/123037Isolation and Characterization of RNA-Containing Exosomes
01/08/123202Isolation of Primary Mouse Trophoblast Cells and Trophoblast Invasion Assay
01/07/123504Intravital Imaging of the Mouse Thymus using 2-Photon Microscopy
01/06/123452High Content Screening in Neurodegenerative Diseases
01/06/123396Mouse Model of Surgically-induced Endometriosis by Auto-transplantation of Uterine Tissue
01/05/122760In vivo Imaging of the Mouse Spinal Cord Using Two-photon Microscopy
01/05/122607Assessing Burrowing, Nest Construction, and Hoarding in Mice
01/04/123612Therapeutic Gene Delivery and Transfection in Human Pancreatic Cancer Cells using Epidermal Growth Factor Receptor-targeted Gelatin Nanoparticles
01/04/123581A Cre-Lox P Recombination Approach for the Detection of Cell Fusion In Vivo
01/03/123686Detection of Toxin Translocation into the Host Cytosol by Surface Plasmon Resonance
01/03/123122Selection of Plasmodium falciparum Parasites for Cytoadhesion to Human Brain Endothelial Cells
01/02/123417How to Measure Cortical Folding from MR Images: a Step-by-Step Tutorial to Compute Local Gyrification Index
01/02/123406A Protocol for Detecting and Scavenging Gas-phase Free Radicals in Mainstream Cigarette Smoke
12/28/113302Single Cell Transcriptional Profiling of Adult Mouse Cardiomyocytes
12/28/113380Selective Viral Transduction of Adult-born Olfactory Neurons for Chronic in vivo Optogenetic Stimulation
12/27/113407Two Types of Assays for Detecting Frog Sperm Chemoattraction
12/27/113603In vivo Electroporation of Morpholinos into the Adult Zebrafish Retina
12/26/113749Identification and Characterization of Protein Glycosylation using Specific Endo- and Exoglycosidases
12/26/113179Quantifying Agonist Activity at G Protein-coupled Receptors
12/23/113432Isolating LacZ-expressing Cells from Mouse Inner Ear Tissues using Flow Cytometry
12/23/113297Evaluation of Cancer Stem Cell Migration Using Compartmentalizing Microfluidic Devices and Live Cell Imaging
12/22/113589Live-cell Imaging of Migrating Cells Expressing Fluorescently-tagged Proteins in a Three-dimensional Matrix
12/22/113227Visualizing Proteins and Macromolecular Complexes by Negative Stain EM: from Grid Preparation to Image Acquisition
12/21/113511Visualization of Vascular Ca2+ Signaling Triggered by Paracrine Derived ROS
12/21/113305MISSION LentiPlex Pooled shRNA Library Screening in Mammalian Cells
12/20/113600Dissection and Culture of Chick Statoacoustic Ganglion and Spinal Cord Explants in Collagen Gels for Neurite Outgrowth Assays
12/20/113118Electrophysiological Measurements and Analysis of Nociception in Human Infants
12/19/113474Direct Detection of the Acetate-forming Activity of the Enzyme Acetate Kinase
12/19/113395Quantitative Comparison of cis-Regulatory Element (CRE) Activities in Transgenic Drosophila melanogaster
12/18/113440Herbivore-induced Blueberry Volatiles and Intra-plant Signaling
12/17/113225Application of a Mouse Ligated Peyer’s Patch Intestinal Loop Assay to Evaluate Bacterial Uptake by M cells
12/16/113521Tracking Hypoxic Signaling within Encapsulated Cell Aggregates
12/16/113478Soft Lithographic Functionalization and Patterning Oxide-free Silicon and Germanium
12/15/113642Assessing Hepatic Metabolic Changes During Progressive Colonization of Germ-free Mouse by 1H NMR Spectroscopy
12/15/113154Recognition of Epidermal Transglutaminase by IgA and Tissue Transglutaminase 2 Antibodies in a Rare Case of Rhesus Dermatitis
12/14/113677Method to Measure Tone of Axial and Proximal Muscle
12/14/113536Skeletal Muscle Gender Dimorphism from Proteomics
12/13/113667DiI-Labeling of DRG Neurons to Study Axonal Branching in a Whole Mount Preparation of Mouse Embryonic Spinal Cord
12/12/113715Multiplexed Fluorometric ImmunoAssay Testing Methodology and Troubleshooting
12/12/113713Mouse Sperm Cryopreservation and Recovery using the I·Cryo Kit
12/10/113437Fluorescence detection methods for microfluidic droplet platforms
12/09/113248Specimen Preparation, Imaging, and Analysis Protocols for Knife-edge Scanning Microscopy
12/09/113189Planar and Three-Dimensional Printing of Conductive Inks
12/08/113438Measurement of Cytosolic Ca2+ in Isolated Contractile Lymphatics
12/08/113382Protocols for Vaginal Inoculation and Sample Collection in the Experimental Mouse Model of Candida vaginitis
12/07/113525Modeling and Imaging 3-Dimensional Collective Cell Invasion
12/07/113246Longitudinal Evaluation of Mouse Hind Limb Bone Loss After Spinal Cord Injury using Novel, in vivo, Methodology
12/06/113404Imaging Neuronal Responses in Slice Preparations of Vomeronasal Organ Expressing a Genetically Encoded Calcium Sensor
12/06/113311Imaging Pheromone Sensing in a Mouse Vomeronasal Acute Tissue Slice Preparation
12/05/113418Scale-Up of Mammalian Cell Culture using a New Multilayered Flask
12/05/113020Isolation of Functional Cardiac Immune Cells
12/04/113176Multifocal Electroretinograms
12/03/112820Cost-effective Method for Microbial Source Tracking Using Specific Human and Animal Viruses
12/02/113560Monitoring Protein Adsorption with Solid-state Nanopores
12/02/113498Synthetic, Multi-Layer, Self-Oscillating Vocal Fold Model Fabrication
12/01/113264Prediction of HIV-1 Coreceptor Usage (Tropism) by Sequence Analysis using a Genotypic Approach
12/01/112770Determination of Molecular Structures of HIV Envelope Glycoproteins using Cryo-Electron Tomography and Automated Sub-tomogram Averaging
11/30/113588Determining the Phagocytic Activity of Clinical Antibody Samples
11/30/112958Cerebrovascular Casting of the Adult Mouse for 3D Imaging and Morphological Analysis
11/29/113505Measuring Fast Calcium Fluxes in Cardiomyocytes
11/29/113203Imaging of Estrogen Receptor-α in Rat Pial Arterioles using a Digital Immunofluorescent Microscope
11/28/113381Electroporation of Craniofacial Mesenchyme
11/28/113148A Quantitative Assay for Insulin-expressing Colony-forming Progenitors
11/27/113348Production and Titering of Recombinant Adeno-associated Viral Vectors
11/26/113602Amide Hydrogen/Deuterium Exchange & MALDI-TOF Mass Spectrometry Analysis of Pak2 Activation
11/25/113559Detection and Isolation of Circulating Melanoma Cells using Photoacoustic Flowmetry
11/25/113366Directed Cellular Self-Assembly to Fabricate Cell-Derived Tissue Rings for Biomechanical Analysis and Tissue Engineering
11/23/113328In Situ Hybridization for the Precise Localization of Transcripts in Plants
11/23/113266Visualization of Mitochondrial Respiratory Function using Cytochrome C Oxidase / Succinate Dehydrogenase (COX/SDH) Double-labeling Histochemistry
11/22/113563Isolation of Mouse Lung Dendritic Cells
11/22/113472Measurement of γHV68 Infection in Mice
11/21/113357Production and Detection of Reactive Oxygen Species (ROS) in Cancers
11/21/113260Implantation of Radiotelemetry Transmitters Yielding Data on ECG, Heart Rate, Core Body Temperature and Activity in Free-moving Laboratory Mice
11/20/112425Microfluidic Device for Recreating a Tumor Microenvironment in Vitro
11/19/113184Mouse Eye Enucleation for Remote High-throughput Phenotyping
11/18/113187Study of the Actin Cytoskeleton in Live Endothelial Cells Expressing GFP-Actin
11/18/113155Cryopreservation of Mouse Embryos by Ethylene Glycol-Based Vitrification
11/17/113659Assessment of GFP Expression and Viability Using the Tali Image-Based Cytometer
11/17/113399Cellular Lipid Extraction for Targeted Stable Isotope Dilution Liquid Chromatography-Mass Spectrometry Analysis
11/16/113337Spheroid Assay to Measure TGF-β-induced Invasion
11/16/113188Hyperinsulinemic-euglycemic Clamps in Conscious, Unrestrained Mice
11/15/113379Using Affordable LED Arrays for Photo-Stimulation of Neurons
11/15/113331Constructing a Low-budget Laser Axotomy System to Study Axon Regeneration in C. elegans
11/14/113457Electrophysiological Characterization of GFP-Expressing Cell Populations in the Intact Retina
11/14/113371Revealing Neural Circuit Topography in Multi-Color
11/13/113125Non-Laser Capture Microscopy Approach for the Microdissection of Discrete Mouse Brain Regions for Total RNA Isolation and Downstream Next-Generation Sequencing and Gene Expression Profiling
11/12/113257Bilaminar Co-culture of Primary Rat Cortical Neurons and Glia
11/11/113258Behavioral Assessment of Manual Dexterity in Non-Human Primates
11/11/113143Quantitative Analysis of Synaptic Vesicle Pool Replenishment in Cultured Cerebellar Granule Neurons using FM Dyes
11/10/113662Immunohistological Labeling of Microtubules in Sensory Neuron Dendrites, Tracheae, and Muscles in the Drosophila Larva Body Wall
11/10/113138In vivo Liver Endocytosis Followed by Purification of Liver Cells by Liver Perfusion
11/09/113307Cross-Modal Multivariate Pattern Analysis
11/09/113280Ice-Cap: A Method for Growing Arabidopsis and Tomato Plants in 96-well Plates for High-Throughput Genotyping
11/08/113384In vitro Uncoating of HIV-1 Cores
11/08/113321Establishment of Epstein-Barr Virus Growth-transformed Lymphoblastoid Cell Lines
11/07/113177Teratoma Generation in the Testis Capsule
11/07/113111Morphological Analysis of Drosophila Larval Peripheral Sensory Neuron Dendrites and Axons Using Genetic Mosaics
11/06/113403Intracranial Implantation with Subsequent 3D In Vivo Bioluminescent Imaging of Murine Gliomas
11/05/113575Stem Cell Transplantation in an in vitro Simulated Ischemia/Reperfusion Model
11/04/113482Labeling Stem Cells with Ferumoxytol, an FDA-Approved Iron Oxide Nanoparticle
11/04/113335A General Method for Evaluating Incubation of Sucrose Craving in Rats
11/03/113274Efficient Derivation of Human Cardiac Precursors and Cardiomyocytes from Pluripotent Human Embryonic Stem Cells with Small Molecule Induction
11/03/113259A Protocol for Computer-Based Protein Structure and Function Prediction
11/02/113519Adhesion Frequency Assay for In Situ Kinetics Analysis of Cross-Junctional Molecular Interactions at the Cell-Cell Interface
11/02/113373Solid-phase Submonomer Synthesis of Peptoid Polymers and their Self-Assembly into Highly-Ordered Nanosheets
11/01/113573Assessing Somatic Hypermutation in Ramos B Cells after Overexpression or Knockdown of Specific Genes
11/01/113400Using Reverse Genetics to Manipulate the NSs Gene of the Rift Valley Fever Virus MP-12 Strain to Improve Vaccine Safety and Efficacy
10/31/112615Quantitatively Measuring In situ Flows using a Self-Contained Underwater Velocimetry Apparatus (SCUVA)
10/31/113336Imaging Odor-Evoked Activities in the Mouse Olfactory Bulb using Optical Reflectance and Autofluorescence Signals
10/30/113633Isolation and Expansion of Human Glioblastoma Multiforme Tumor Cells Using the Neurosphere Assay
10/29/113195Flash Photolysis of Caged Compounds in the Cilia of Olfactory Sensory Neurons
10/28/113273Efficient Derivation of Human Neuronal Progenitors and Neurons from Pluripotent Human Embryonic Stem Cells with Small Molecule Induction
10/28/113250Performing Custom MicroRNA Microarray Experiments
10/27/113340Single Read and Paired End mRNA-Seq Illumina Libraries from 10 Nanograms Total RNA
10/27/113255Visualization of DNA Replication in the Vertebrate Model System DT40 using the DNA Fiber Technique
10/26/113159Isolation & Characterization of Hoechstlow CD45negative Mouse Lung Mesenchymal Stem Cells
10/26/112969Robust Generation of Hepatocyte-like Cells from Human Embryonic Stem Cell Populations
10/25/113334Heterogeneity Mapping of Protein Expression in Tumors using Quantitative Immunofluorescence
10/25/113201Experimental Generation of Carcinoma-Associated Fibroblasts (CAFs) from Human Mammary Fibroblasts
10/24/113314GENPLAT: an Automated Platform for Biomass Enzyme Discovery and Cocktail Optimization
10/24/113214Chromatographic Purification of Highly Active Yeast Ribosomes
10/23/113344Multiplex Detection of Bacteria in Complex Clinical and Environmental Samples using Oligonucleotide-coupled Fluorescent Microspheres
10/22/113550Functional Calcium Imaging in Developing Cortical Networks
10/21/113170DNA Methylation: Bisulphite Modification and Analysis
10/21/113132Neural Tube Closure in Mouse Whole Embryo Culture
10/20/113252Video-rate Scanning Confocal Microscopy and Microendoscopy
10/20/112967Oral Biofilm Analysis of Palatal Expanders by Fluorescence In-Situ Hybridization and Confocal Laser Scanning Microscopy
10/19/113282Recording Large-scale Neuronal Ensembles with Silicon Probes in the Anesthetized Rat
10/19/113238The Trier Social Stress Test Protocol for Inducing Psychological Stress
10/18/113108Quantifying Cognitive Decrements Caused by Cranial Radiotherapy
10/18/113107Stem Cell Transplantation Strategies for the Restoration of Cognitive Dysfunction Caused by Cranial Radiotherapy
10/17/113244Monitoring Equilibrium Changes in RNA Structure by 'Peroxidative' and 'Oxidative' Hydroxyl Radical Footprinting
10/17/113129Tracking Morphogenetic Tissue Deformations in the Early Chick Embryo
10/16/113270RNAi Mediated Gene Knockdown and Transgenesis by Microinjection in the Necromenic Nematode Pristionchus pacificus
10/15/113167Procedures for Rat in situ Skeletal Muscle Contractile Properties
10/14/113363Following Cell-fate in E. coli After Infection by Phage Lambda
10/14/112950Microwave Assisted Rapid Diagnosis of Plant Virus Diseases by Transmission Electron Microscopy
10/13/113405Stretching Short Sequences of DNA with Constant Force Axial Optical Tweezers
10/13/113089Organotypic Collagen I Assay: A Malleable Platform to Assess Cell Behaviour in a 3-Dimensional Context
10/12/113556VisioTracker, an Innovative Automated Approach to Oculomotor Analysis
10/12/112723Impulsive Pressurization of Neuronal Cells for Traumatic Brain Injury Study
10/11/113245In vivo Dual Substrate Bioluminescent Imaging
10/11/113166A Swine Model of Neonatal Asphyxia
10/10/113183Isolation of CD133+ Liver Stem Cells for Clonal Expansion
10/10/112797Whole Mount in Situ Hybridization of E8.5 to E11.5 Mouse Embryos
10/09/113483Propagating and Detecting an Infectious Molecular Clone of Maedi-visna Virus that Expresses Green Fluorescent Protein
10/08/113443Using MazeSuite and Functional Near Infrared Spectroscopy to Study Learning in Spatial Navigation
10/07/113267A Method for Labeling Vasculature in Embryonic Mice
10/07/113140Dissecting Host-virus Interaction in Lytic Replication of a Model Herpesvirus
10/06/113109Mapping Inhibitory Neuronal Circuits by Laser Scanning Photostimulation
10/06/113083Investigating the Neural Mechanisms of Aware and Unaware Fear Memory with fMRI
10/05/113172Single Cell Fate Mapping in Zebrafish
10/05/113110Transfecting and Nucleofecting Human Induced Pluripotent Stem Cells
10/04/113441Synthesis, Assembly, and Characterization of Monolayer Protected Gold Nanoparticle Films for Protein Monolayer Electrochemistry
10/04/113364Adaptation of a Haptic Robot in a 3T fMRI
10/03/113175In vivo Bioluminescence Imaging of Tumor Hypoxia Dynamics of Breast Cancer Brain Metastasis in a Mouse Model
10/03/112968Delivery of Therapeutic Agents Through Intracerebroventricular (ICV) and Intravenous (IV) Injection in Mice
10/02/113139Drosophila Pupal Abdomen Immunohistochemistry
10/01/113162Experimental Manipulation of Body Size to Estimate Morphological Scaling Relationships in Drosophila
09/30/113190Purification of Hsp104, a Protein Disaggregase
09/30/113165Heart Dissection in Larval, Juvenile and Adult Zebrafish, Danio rerio
09/29/113146Separation of Single-stranded DNA, Double-stranded DNA and RNA from an Environmental Viral Community Using Hydroxyapatite Chromatography
09/29/113030Studying Age-dependent Genomic Instability using the S. cerevisiae Chronological Lifespan Model
09/28/113343Identifying Targets of Human microRNAs with the LightSwitch Luciferase Assay System using 3'UTR-reporter Constructs and a microRNA Mimic in Adherent Cells
09/28/113293RNA Isolation of Pseudomonas aeruginosa Colonizing the Murine Gastrointestinal Tract
09/27/113136Profiling Voltage-gated Potassium Channel mRNA Expression in Nigral Neurons using Single-cell RT-PCR Techniques
09/27/113390Utilization of Plasmonic and Photonic Crystal Nanostructures for Enhanced Micro- and Nanoparticle Manipulation
09/26/113157A Simple Guide Screw Method for Intracranial Xenograft Studies in Mice
09/26/112960Amplifying and Quantifying HIV-1 RNA in HIV Infected Individuals with Viral Loads Below the Limit of Detection by Standard Clinical Assays
09/25/113322Colorectal Cancer Cell Surface Protein Profiling Using an Antibody Microarray and Fluorescence Multiplexing
09/24/113296Tracking Neutrophil Intraluminal Crawling, Transendothelial Migration and Chemotaxis in Tissue by Intravital Video Microscopy
09/23/113150Quantitation and Analysis of the Formation of HO-Endonuclease Stimulated Chromosomal Translocations by Single-Strand Annealing in Saccharomyces cerevisiae
09/23/113096Detection of Histone Modifications in Plant Leaves
09/22/113119Mesenteric Artery Contraction and Relaxation Studies Using Automated Wire Myography
09/22/113010A High-throughput Automated Platform for the Development of Manufacturing Cell Lines for Protein Therapeutics
09/21/113060Automated Hydrophobic Interaction Chromatography Column Selection for Use in Protein Purification
09/21/113059Biochemical Reconstitution of Steroid Receptor•Hsp90 Protein Complexes and Reactivation of Ligand Binding
09/20/113398Formulation of Diblock Polymeric Nanoparticles through Nanoprecipitation Technique
09/20/113128Imaging G-protein Coupled Receptor (GPCR)-mediated Signaling Events that Control Chemotaxis of Dictyostelium Discoideum
09/19/113251Encapsulation of Cardiomyocytes in a Fibrin Hydrogel for Cardiac Tissue Engineering
09/19/113173Using a Comparative Species Approach to Investigate the Neurobiology of Paternal Responses
09/18/113069Intraspinal Cell Transplantation for Targeting Cervical Ventral Horn in Amyotrophic Lateral Sclerosis and Traumatic Spinal Cord Injury
09/17/113196Preparation of Synaptoneurosomes from Mouse Cortex using a Discontinuous Percoll-Sucrose Density Gradient
09/16/113473Detection of Protein Interactions in Plant using a Gateway Compatible Bimolecular Fluorescence Complementation (BiFC) System
09/16/113263Basophil Activation Test for Investigation of IgE-Mediated Mechanisms in Drug Hypersensitivity
09/15/113200Lectin-based Isolation and Culture of Mouse Embryonic Motoneurons
09/15/112840Preparing Undercut Model of Posttraumatic Epileptogenesis in Rodents
09/14/113332A Functional Whole Blood Assay to Measure Viability of Mycobacteria, using Reporter-Gene Tagged BCG or M.Tb (BCG lux/M.Tb lux)
09/14/113087Biophysical Assays to Probe the Mechanical Properties of the Interphase Cell Nucleus: Substrate Strain Application and Microneedle Manipulation
09/13/113275Optical Mapping of Action Potentials and Calcium Transients in the Mouse Heart
09/13/113160Multiparametric Optical Mapping of the Langendorff-perfused Rabbit Heart
09/12/113309Changing the Direction and Orientation of Electric Field During Electric Pulses Application Improves Plasmid Gene Transfer in vitro
09/12/113178Probing the Brain in Autism Using fMRI and Diffusion Tensor Imaging
09/11/113236X-ray Dose Reduction through Adaptive Exposure in Fluoroscopic Imaging
09/10/112855Bromodeoxyuridine (BrdU) Labeling and Subsequent Fluorescence Activated Cell Sorting for Culture-independent Identification of Dissolved Organic Carbon-degrading Bacterioplankton
09/09/113197Autologous Endothelial Progenitor Cell-Seeding Technology and Biocompatibility Testing For Cardiovascular Devices in Large Animal Model
09/09/113182Expansion of Human Peripheral Blood γδ T Cells using Zoledronate
09/08/113124Subcutaneous Administration of Muscarinic Antagonists and Triple-Immunostaining of the Levator Auris Longus Muscle in Mice
09/08/112806Examining Local Network Processing using Multi-contact Laminar Electrode Recording
09/07/113040A Matrigel-Based Tube Formation Assay to Assess the Vasculogenic Activity of Tumor Cells
09/07/112796Isothermal Titration Calorimetry for Measuring Macromolecule-Ligand Affinity
09/06/113164Creating Two-Dimensional Patterned Substrates for Protein and Cell Confinement
09/06/112767Diagnosis of Ecto- and Endoparasites in Laboratory Rats and Mice
09/05/113208Accurate and Simple Evaluation of Vascular Anastomoses in Monochorionic Placenta using Colored Dye
09/05/112837Guide Wire Assisted Catheterization and Colored Dye Injection for Vascular Mapping of Monochorionic Twin Placentas
09/04/112897Mutagenesis and Analysis of Genetic Mutations in the GC-rich KISS1 Receptor Sequence Identified in Humans with Reproductive Disorders
09/03/113027Preparation of Quality Inositol Pyrophosphates
09/02/113261Use of the EpiAirway Model for Characterizing Long-term Host-pathogen Interactions
09/02/113241Introducing Shear Stress in the Study of Bacterial Adhesion
09/01/113446Habituation and Prepulse Inhibition of Acoustic Startle in Rodents
09/01/113126Live Imaging of Dorsal Root Axons after Rhizotomy
08/31/113058Pharmacological and Functional Genetic Assays to Manipulate Regeneration of the Planarian Dugesia japonica
08/31/112857ReAsH/FlAsH Labeling and Image Analysis of Tetracysteine Sensor Proteins in Cells
08/30/112715Examining the Characteristics of Episodic Memory using Event-related Potentials in Patients with Alzheimer's Disease
08/30/113116Normothermic Cardiac Arrest and Cardiopulmonary Resuscitation: A Mouse Model of Ischemia-Reperfusion Injury
08/29/112845Laser Ablation of the Zebrafish Pronephros to Study Renal Epithelial Regeneration
08/29/112839Dissection of the Adult Zebrafish Kidney
08/28/112911Micro-Mechanical Characterization of Lung Tissue Using Atomic Force Microscopy
08/27/112959Preparation of Adult Drosophila Eyes for Thin Sectioning and Microscopic Analysis
08/26/112430Brain Imaging Investigation of the Neural Correlates of Emotion Regulation
08/26/112396Brain Imaging Investigation of the Neural Correlates of Emotional Autobiographical Recollection
08/25/112898Measuring the Kinetics of mRNA Transcription in Single Living Cells
08/25/112852Time-lapse Microscopy of Early Embryogenesis in Caenorhabditis elegans
08/24/112948Biochemical Measurement of Neonatal Hypoxia
08/24/112618Autologous Blood Injection to Model Spontaneous Intracerebral Hemorrhage in Mice
08/23/113316Design of a Cyclic Pressure Bioreactor for the Ex Vivo Study of Aortic Heart Valves
08/23/113221Imaging Leukocyte Adhesion to the Vascular Endothelium at High Intraluminal Pressure
08/22/113063Lateral Fluid Percussion: Model of Traumatic Brain Injury in Mice
08/22/112762Isolating Nasal Olfactory Stem Cells from Rodents or Humans
08/21/113324Derivation of Enriched Oligodendrocyte Cultures and Oligodendrocyte/Neuron Myelinating Co-cultures from Post-natal Murine Tissues
08/20/112938Agrobacterium-Mediated Virus-Induced Gene Silencing Assay In Cotton
08/19/112912A High Throughput in situ Hybridization Method to Characterize mRNA Expression Patterns in the Fetal Mouse Lower Urogenital Tract
08/19/112882Single Drosophila Ommatidium Dissection and Imaging
08/18/113092The Spared Nerve Injury (SNI) Model of Induced Mechanical Allodynia in Mice
08/18/113077A Mouse Model of the Cornea Pocket Assay for Angiogenesis Study
08/17/113181Lensless Fluorescent Microscopy on a Chip
08/17/113149Cell-based Calcium Assay for Medium to High Throughput Screening of TRP Channel Functions using FlexStation 3
08/16/113191Cercarial Transformation and in vitro Cultivation of Schistosoma mansoni Schistosomules
08/16/113151Preparation of Viral DNA from Nucleocapsids
08/15/113024Mouse in Utero Electroporation: Controlled Spatiotemporal Gene Transfection
08/15/112964Targeting Olfactory Bulb Neurons Using Combined In Vivo Electroporation and Gal4-Based Enhancer Trap Zebrafish Lines
08/14/112961Protein Membrane Overlay Assay: A Protocol to Test Interaction Between Soluble and Insoluble Proteins in vitro
08/13/112942High Throughput Screening of Fungal Endoglucanase Activity in Escherichia coli
08/12/112899Induction and Testing of Hypoxia in Cell Culture
08/12/112716Mammary Transplantation of Stromal Cells and Carcinoma Cells in C57BL/6J Mice
08/11/112864Competitive Genomic Screens of Barcoded Yeast Libraries
08/11/112914An In Vitro System to Study Tumor Dormancy and the Switch to Metastatic Growth
08/10/112880Bacterial Immobilization for Imaging by Atomic Force Microscopy
08/10/112753Axon Stretch Growth: The Mechanotransduction of Neuronal Growth
08/09/113076Measuring Bacterial Load and Immune Responses in Mice Infected with Listeria monocytogenes
08/09/113041Structure of HIV-1 Capsid Assemblies by Cryo-electron Microscopy and Iterative Helical Real-space Reconstruction
08/08/113007Haptic/Graphic Rehabilitation: Integrating a Robot into a Virtual Environment Library and Applying it to Stroke Therapy
08/08/112835Targeted Training of Ultrasonic Vocalizations in Aged and Parkinsonian Rats
08/07/112966Diagnostic Necropsy and Selected Tissue and Sample Collection in Rats and Mice
08/06/112781Multiplex PCR and Reverse Line Blot Hybridization Assay (mPCR/RLB)
08/05/113289FSL Constructs: A Simple Method for Modifying Cell/Virion Surfaces with a Range of Biological Markers Without Affecting their Viability
08/05/112809Identification and Analysis of Mouse Erythroid Progenitors using the CD71/TER119 Flow-cytometric Assay
08/05/112764Cryopreservation of Preimplantation Embryos of Cattle, Sheep, and Goats
08/04/112644Establishment and Propagation of Human Retinoblastoma Tumors in Immune Deficient Mice
08/04/112257In vivo Near Infrared Fluorescence (NIRF) Intravascular Molecular Imaging of Inflammatory Plaque, a Multimodal Approach to Imaging of Atherosclerosis
08/03/112937The Three-Dimensional Human Skin Reconstruct Model: a Tool to Study Normal Skin and Melanoma Progression
08/03/112552High Throughput MicroRNA Profiling: Optimized Multiplex qRT-PCR at Nanoliter Scale on the Fluidigm Dynamic ArrayTM IFCs
08/02/112832Avian Influenza Surveillance with FTA Cards: Field Methods, Biosafety, and Transportation Issues Solved
08/02/112829Rapid Diagnosis of Avian Influenza Virus in Wild Birds: Use of a Portable rRT-PCR and Freeze-dried Reagents in the Field
08/01/112949Multi-electrode Array Recordings of Neuronal Avalanches in Organotypic Cultures
08/01/112823Mosaic Analysis of Gene Function in Postnatal Mouse Brain Development by Using Virus-based Cre Recombination
07/31/112812Quantification of Proteins Using Peptide Immunoaffinity Enrichment Coupled with Mass Spectrometry
07/30/112962Isolation of Human Islets from Partially Pancreatectomized Patients
07/30/112777Determining Genetic Expression Profiles in C. elegans Using Microarray and Real-time PCR
07/29/113103High-Resolution Endocardial and Epicardial Optical Mapping in a Sheep Model of Stretch-Induced Atrial Fibrillation
07/29/113085Long-term Culture of Human Breast Cancer Specimens and Their Analysis Using Optical Projection Tomography
07/29/112846Method for Novel Anti-Cancer Drug Development using Tumor Explants of Surgical Specimens
07/28/113145Live Cell Imaging of Bacillus subtilis and Streptococcus pneumoniae using Automated Time-lapse Microscopy
07/28/113123Use of an Optical Trap for Study of Host-Pathogen Interactions for Dynamic Live Cell Imaging
07/27/112836High-throughput Yeast Plasmid Overexpression Screen
07/27/112802Visualization of the Interstitial Cells of Cajal (ICC) Network in Mice
07/26/112831Detection of Neuritic Plaques in Alzheimer's Disease Mouse Model
07/26/112807A Rapid Approach to High-Resolution Fluorescence Imaging in Semi-Thick Brain Slices
07/25/113039The Application Of Permanent Middle Cerebral Artery Ligation in the Mouse
07/25/112941Multi-photon Imaging of Tumor Cell Invasion in an Orthotopic Mouse Model of Oral Squamous Cell Carcinoma
07/24/112822Transduction of Human Cells with Polymer-complexed Ecotropic Lentivirus for Enhanced Biosafety
07/23/112851Mouse Oocyte Microinjection, Maturation and Ploidy Assessment
07/22/113130An Allelotyping PCR for Identifying Salmonella enterica serovars Enteritidis, Hadar, Heidelberg, and Typhimurium
07/22/113071Human In Vitro Suppression as Screening Tool for the Recognition of an Early State of Immune Imbalance
07/21/112844Preparation, Purification, and Characterization of Lanthanide Complexes for Use as Contrast Agents for Magnetic Resonance Imaging
07/21/112828Evaluation of Mammary Gland Development and Function in Mouse Models
07/20/112920Morris Water Maze Test for Learning and Memory Deficits in Alzheimer's Disease Model Mice
07/20/112803Chromatin Immunoprecipitation from Dorsal Root Ganglia Tissue following Axonal Injury
07/19/112502Quantitative Autonomic Testing
07/19/112549Use of a Hanging-weight System for Isolated Renal Artery Occlusion
07/18/113061Visualization of Recombinant DNA and Protein Complexes Using Atomic Force Microscopy
07/18/112965Investigating Outer Hair Cell Motility with a Combination of External Alternating Electrical Field Stimulation and High-speed Image Analysis
07/18/112779A Fluorescence Microscopy Assay for Monitoring Mitophagy in the Yeast Saccharomyces cerevisiae
07/17/112858GABA-activated Single-channel and Tonic Currents in Rat Brain Slices
07/16/112813Lentiviral-mediated Knockdown During Ex Vivo Erythropoiesis of Human Hematopoietic Stem Cells
07/15/113104DNA Fingerprinting of Mycobacterium leprae Strains Using Variable Number Tandem Repeat (VNTR) - Fragment Length Analysis (FLA)
07/15/113054Ex vivo Imaging of T Cells in Murine Lymph Node Slices with Widefield and Confocal Microscopes
07/14/112799In vitro Reconstitution of the Active T. castaneum Telomerase
07/14/112790Quantifying the Frequency of Tumor-propagating Cells Using Limiting Dilution Cell Transplantation in Syngeneic Zebrafish
07/13/112841Screening Assay for Oxidative Stress in a Feline Astrocyte Cell Line, G355-5
07/13/112794Analysis of Dendritic Spine Morphology in Cultured CNS Neurons
07/12/112737Electrolytic Inferior Vena Cava Model (EIM) of Venous Thrombosis
07/12/112523Mouse Bladder Wall Injection
07/11/113144Increasing cDNA Yields from Single-cell Quantities of mRNA in Standard Laboratory Reverse Transcriptase Reactions using Acoustic Microstreaming
07/11/113065Bioengineering Human Microvascular Networks in Immunodeficient Mice
07/10/112834Surgical Transplantation of Mouse Neural Stem Cells into the Spinal Cords of Mice Infected with Neurotropic Mouse Hepatitis Virus
07/09/113168Visualizing Dengue Virus through Alexa Fluor Labeling
07/08/112766Optimized Protocol for Efficient Transfection of Dendritic Cells without Cell Maturation
07/08/112740'Bioluminescent' Reporter Phage for the Detection of Category A Bacterial Pathogens
07/07/112887Extraction of High Molecular Weight DNA from Microbial Mats
07/07/112730Live Cell Calcium Imaging Combined with siRNA Mediated Gene Silencing Identifies Ca2+ Leak Channels in the ER Membrane and their Regulatory Mechanisms
07/06/112588Focussed Ion Beam Milling and Scanning Electron Microscopy of Brain Tissue
07/06/112379Brain Imaging Investigation of the Neural Correlates of Observing Virtual Social Interactions
07/05/112590Microvascular Decompression: Salient Surgical Principles and Technical Nuances
07/05/112281Reverse Total Shoulder Arthroplasty
07/04/112903Shape Memory Polymers for Active Cell Culture
07/04/112896Fluorescent Nanoparticles for the Measurement of Ion Concentration in Biological Systems
07/03/113240High-throughput Saccharification Assay for Lignocellulosic Materials
07/02/112901An Optimized Protocol for Rearing Fopius arisanus, a Parasitoid of Tephritid Fruit Flies
07/01/112848Flow Cytometry Analysis of Immune Cells Within Murine Aortas
07/01/112805Immuno-fluorescence Assay of Leptospiral Surface-exposed Proteins
06/30/112722Insulin Injection and Hemolymph Extraction to Measure Insulin Sensitivity in Adult Drosophila melanogaster
06/30/112717Visualization of MG53-mediated Cell Membrane Repair Using in vivo and in vitro Systems
06/29/112773Activation of Apoptosis by Cytoplasmic Microinjection of Cytochrome c
06/29/112696Photobleaching Assays (FRAP & FLIP) to Measure Chromatin Protein Dynamics in Living Embryonic Stem Cells
06/28/112821Quantifying the Activity of cis-Regulatory Elements in the Mouse Retina by Explant Electroporation
06/28/112755Microwave-assisted One-pot Synthesis of N-succinimidyl-4-[18F]fluorobenzoate ([18F]SFB)
06/27/112759Investigating Social Cognition in Infants and Adults Using Dense Array Electroencephalography (dEEG)
06/27/112742A Cell Free Assay System Estimating the Neutralizing Capacity of GM-CSF Antibody using Recombinant Soluble GM-CSF Receptor
06/27/112689Live Imaging of Cell Extrusion from the Epidermis of Developing Zebrafish
06/26/112652Basic Surgical Techniques in the Göttingen Minipig: Intubation, Bladder Catheterization, Femoral Vessel Catheterization, and Transcardial Perfusion
06/25/112854Ex Vivo Infection of Live Tissue with Oncolytic Viruses
06/24/113094Antimicrobial Susceptibility Testing of Mycobacterium Tuberculosis Complex for First and Second Line Drugs by Broth Dilution in a Microtiter Plate Format
06/24/112847In vivo Electroporation of Developing Mouse Retina
06/23/112957Efficient Gene Delivery into Multiple CNS Territories Using In Utero Electroporation
06/23/112954Development of Cell-type specific anti-HIV gp120 aptamers for siRNA delivery
06/22/112789Preparation of Living Isolated Vertebrate Photoreceptor Cells for Fluorescence Imaging
06/22/112713A System for Culturing Iris Pigment Epithelial Cells to Study Lens Regeneration in Newt
06/21/112808Evaluation of Nanoparticle Uptake in Tumors in Real Time Using Intravital Imaging
06/21/112688High Resolution 3D Imaging of Ex-Vivo Biological Samples by Micro CT
06/20/113057Optimized PCR-based Detection of Mycoplasma
06/20/112804Alginate Hydrogels for Three-Dimensional Organ Culture of Ovaries and Oviducts
06/19/113047Characterizing Herbivore Resistance Mechanisms: Spittlebugs on Brachiaria spp. as an Example
06/18/113078Rat Mesentery Angiogenesis Assay
06/17/112793Vibratome Sectioning for Enhanced Preservation of the Cytoarchitecture of the Mammalian Organ of Corti
06/17/112599Neuromodulation and Mitochondrial Transport: Live Imaging in Hippocampal Neurons over Long Durations
06/16/112953Isolation of Fidelity Variants of RNA Viruses and Characterization of Virus Mutation Frequency
06/16/112724Methods to Assess Beta Cell Death Mediated by Cytotoxic T Lymphocytes
06/15/112738Mouse Complete Stasis Model of Inferior Vena Cava Thrombosis
06/15/112534A Strategy to Identify de Novo Mutations in Common Disorders such as Autism and Schizophrenia
06/14/113115Plasma Lithography Surface Patterning for Creation of Cell Networks
06/14/112646Engineering Biological-Based Vascular Grafts Using a Pulsatile Bioreactor
06/13/112910Modeling Neural Immune Signaling of Episodic and Chronic Migraine Using Spreading Depression In Vitro
06/13/112747Isolation of Brain-infiltrating Leukocytes
06/12/112963Introduction to the Ultrasound Targeted Microbubble Destruction Technique
06/11/112830Sequencing of Bacterial Microflora in Peripheral Blood: our Experience with HIV-infected Patients
06/10/112900Microiontophoresis and Micromanipulation for Intravital Fluorescence Imaging of the Microcirculation
06/10/112874The Mouse Cremaster Muscle Preparation for Intravital Imaging of the Microcirculation
06/09/112889Detection of Infectious Virus from Field-collected Mosquitoes by Vero Cell Culture Assay
06/09/112682ampliPHOX Colorimetric Detection on a DNA Microarray for Influenza
06/08/112664Characterizing Bacterial Volatiles using Secondary Electrospray Ionization Mass Spectrometry (SESI-MS)
06/08/112660High-Throughput Measurement and Classification of Organic P in Environmental Samples
06/07/112581Coronary Artery Ligation and Intramyocardial Injection in a Murine Model of Infarction
06/07/112559Modified ES / OP9 Co-Culture Protocol Provides Enhanced Characterization of Hematopoietic Progeny
06/06/112686Imaging Glycans in Zebrafish Embryos by Metabolic Labeling and Bioorthogonal Click Chemistry
06/06/112068Fixed Volume or Fixed Pressure: A Murine Model of Hemorrhagic Shock
06/05/112909Low-Cost Cryo-Light Microscopy Stage Fabrication for Correlated Light/Electron Microscopy
06/04/113080Whole Mount Preparation of the Adult Drosophila Ventral Nerve Cord for Giant Fiber Dye Injection
06/03/112649Mapping and Application of Enhancer-trap Flippase Expression in Larval and Adult Drosophila CNS
06/03/112600Whole Cell Recording from an Organotypic Slice Preparation of Neocortex
06/02/112785Generation and Labeling of Murine Bone Marrow-derived Dendritic Cells with Qdot Nanocrystals for Tracking Studies
06/02/112659Direct Observation of Phagocytosis and NET-formation by Neutrophils in Infected Lungs using 2-photon Microscopy
06/01/112922Fabrication of Electrochemical-DNA Biosensors for the Reagentless Detection of Nucleic Acids, Proteins and Small Molecules
06/01/112605Bioluminescence Imaging for Assessment of Immune Responses Following Implantation of Engineered Heart Tissue (EHT)
05/31/112703Using SCOPE to Identify Potential Regulatory Motifs in Coregulated Genes
05/31/112645Analyzing the Function of Small GTPases by Microinjection of Plasmids into Polarized Epithelial Cells
05/30/112815Quantitative Analysis of Cancer Metastasis using an Avian Embryo Model
05/30/112712Magnetic Resonance Imaging Quantification of Pulmonary Perfusion using Calibrated Arterial Spin Labeling
05/29/112627Examining the Conformational Dynamics of Membrane Proteins in situ with Site-directed Fluorescence Labeling
05/28/112701Solid Plate-based Dietary Restriction in Caenorhabditis elegans
05/27/112736Generation of Multivirus-specific T Cells to Prevent/treat Viral Infections after Allogeneic Hematopoietic Stem Cell Transplant
05/27/112706Live-cell Imaging of Sensory Organ Precursor Cells in Intact Drosophila Pupae
05/26/112700Polarized Translocation of Fluorescent Proteins in Xenopus Ectoderm in Response to Wnt Signaling
05/26/112721Transmembrane Domain Oligomerization Propensity determined by ToxR Assay
05/25/112752Vibrodissociation of Neurons from Rodent Brain Slices to Study Synaptic Transmission and Image Presynaptic Terminals
05/25/112681Generation of Neural Stem Cells from Discarded Human Fetal Cortical Tissue
05/24/112757A Simple and Efficient Method to Isolate Macrophages from Mixed Primary Cultures of Adult Liver Cells
05/24/112774Trichuris muris Infection: A Model of Type 2 Immunity and Inflammation in the Gut
05/23/112670Coherence between Brain Cortical Function and Neurocognitive Performance during Changed Gravity Conditions
05/23/112704Determination of Mitochondrial Membrane Potential and Reactive Oxygen Species in Live Rat Cortical Neurons
05/23/112744Electrode Positioning and Montage in Transcranial Direct Current Stimulation
05/22/112951Lentivirus-mediated Genetic Manipulation and Visualization of Olfactory Sensory Neurons in vivo
05/21/112692Creation of Reversible Cholestatic Rat Model
05/20/112620Whole-mount Immunohistochemical Analysis for Embryonic Limb Skin Vasculature: a Model System to Study Vascular Branching Morphogenesis in Embryo
05/20/112572Swimming Performance Assessment in Fishes
05/19/112745High-throughput Screening and Biosensing with Fluorescent C. elegans Strains
05/19/112707In vivo Laser Axotomy in C. elegans
05/18/112725Transplantation of Cells Directly into the Kidney of Adult Zebrafish
05/18/112592Rejection of Fluorescence Background in Resonance and Spontaneous Raman Microspectroscopy
05/17/112613Hyponeophagia: A Measure of Anxiety in the Mouse
05/17/112564Organotypic Cerebellar Cultures: Apoptotic Challenges and Detection
05/16/112783In Vitro Assay of Bacterial Adhesion onto Mammalian Epithelial Cells
05/16/112772A Protocol for Collecting and Staining Hemocytes from the Yellow Fever Mosquito Aedes aegypti
05/15/112778Gene Transfer for Ischemic Heart Failure in a Preclinical Model
05/14/113074Cannulation of the Mouse Submandibular Salivary Gland via the Wharton's Duct
05/13/112641Isolation of Drosophila melanogaster Testes
05/13/112537Dissection and Staining of Drosophila Larval Ovaries
05/12/112261Methods for Experimental Manipulations after Optic Nerve Transection in the Mammalian CNS
05/12/112241Optic Nerve Transection: A Model of Adult Neuron Apoptosis in the Central Nervous System
05/11/112718Microsurgical Venous Pouch Arterial-Bifurcation Aneurysms in the Rabbit Model: Technical Aspects
05/11/112782An in vivo Rodent Model of Contraction-induced Injury and Non-invasive Monitoring of Recovery
05/10/112710Seven Steps to Stellate Cells
05/10/112687Use of Fluorescent Immuno-Chemistry for the detection of Edwardsiella ictaluri in channel catfish (I. punctatus) samples
05/09/112728Ex Vivo Culture of Primary Human Fallopian Tube Epithelial Cells
05/09/112663Genomic MRI - a Public Resource for Studying Sequence Patterns within Genomic DNA
05/08/112879Intraluminal Middle Cerebral Artery Occlusion (MCAO) Model for Ischemic Stroke with Laser Doppler Flowmetry Guidance in Mice
05/07/112860Cecal Ligation Puncture Procedure
05/06/112720A Simple Hanging Drop Cell Culture Protocol for Generation of 3D Spheroids
05/06/112680A β-glucuronidase (GUS) Based Cell Death Assay
05/05/112679Quantitative Visualization and Detection of Skin Cancer Using Dynamic Thermal Imaging
05/05/112525Pressure Controlled Ventilation to Induce Acute Lung Injury in Mice
05/04/112433Brain Imaging Investigation of the Memory-Enhancing Effect of Emotion
05/04/111694Cerebral Blood Oxygenation Measurement Based on Oxygen-dependent Quenching of Phosphorescence
05/03/112729Three-dimensional Optical-resolution Photoacoustic Microscopy
05/03/112694Biomolecular Detection employing the Interferometric Reflectance Imaging Sensor (IRIS)
05/02/112702Non-surgical Intratracheal Instillation of Mice with Analysis of Lungs and Lung Draining Lymph Nodes by Flow Cytometry
05/02/112444Protocol for Recombinant RBD-based SARS Vaccines: Protein Preparation, Animal Vaccination and Neutralization Detection
05/01/113084IgY Technology: Extraction of Chicken Antibodies from Egg Yolk by Polyethylene Glycol (PEG) Precipitation
04/30/112638iCLIP - Transcriptome-wide Mapping of Protein-RNA Interactions with Individual Nucleotide Resolution
04/29/112690Characterization of the Isolated, Ventilated, and Instrumented Mouse Lung Perfused with Pulsatile Flow
04/29/112677Chromatin Immunoprecipitation Assay for Tissue-specific Genes using Early-stage Mouse Embryos
04/28/112672Lineage Labeling of Zebrafish Cells with Laser Uncagable Fluorescein Dextran
04/28/112669Thermodynamics of Membrane Protein Folding Measured by Fluorescence Spectroscopy
04/27/112676Dissection and Imaging of Active Zones in the Drosophila Neuromuscular Junction
04/27/112536Using a Pan-Viral Microarray Assay (Virochip) to Screen Clinical Samples for Viral Pathogens
04/26/112657Lipid Vesicle-mediated Affinity Chromatography using Magnetic Activated Cell Sorting (LIMACS): a Novel Method to Analyze Protein-lipid Interaction
04/26/112593Detection of Post-translational Modifications on Native Intact Nucleosomes by ELISA
04/25/112685An Optic Nerve Crush Injury Murine Model to Study Retinal Ganglion Cell Survival
04/25/112634Transcriptome Analysis of Single Cells
04/24/112597Modified Annexin V/Propidium Iodide Apoptosis Assay For Accurate Assessment of Cell Death
04/23/112621Associated Chromosome Trap for Identifying Long-range DNA Interactions
04/22/112656Modified Mouse Embryonic Stem Cell based Assay for Quantifying Cardiogenic Induction Efficiency
04/22/112555Isolation and Culture of Cells from the Nephrogenic Zone of the Embryonic Mouse Kidney
04/21/112650Physiological, Morphological and Neurochemical Characterization of Neurons Modulated by Movement
04/21/112583Migratory Behavior of Cells Generated in Ganglionic Eminence Cultures
04/20/112824Particle Agglutination Method for Poliovirus Identification
04/20/112732A Calcium Bioluminescence Assay for Functional Analysis of Mosquito (Aedes aegypti) and Tick (Rhipicephalus microplus) G Protein-coupled Receptors
04/19/112560Efficient Polyethylene Glycol (PEG) Mediated Transformation of the Moss Physcomitrella patens
04/19/112526Use of a Hanging Weight System for Coronary Artery Occlusion in Mice
04/18/112148Methods to Quantify Pharmacologically Induced Alterations in Motor Function in Human Incomplete SCI
04/18/112074Pseudofracture: An Acute Peripheral Tissue Trauma Model
04/17/112678Transfection of Mouse Retinal Ganglion Cells by in vivo Electroporation
04/16/112568Fluorescence Recovery After Photobleaching (FRAP) of Fluorescence Tagged Proteins in Dendritic Spines of Cultured Hippocampal Neurons
04/15/112643Bimolecular Fluorescence Complementation
04/15/112602Flow Cytometry Purification of Mouse Meiotic Cells
04/14/112775Monitoring Tumor Metastases and Osteolytic Lesions with Bioluminescence and Micro CT Imaging
04/14/112357Using Bioluminescent Imaging to Investigate Synergism Between Streptococcus pneumoniae and Influenza A Virus in Infant Mice
04/13/112642Electrophysiology of Scorpion Peg Sensilla
04/13/112629Studying the Neural Basis of Adaptive Locomotor Behavior in Insects
04/13/112096A Method for Murine Islet Isolation and Subcapsular Kidney Transplantation
04/12/112632Manual Muscle Testing: A Method of Measuring Extremity Muscle Strength Applied to Critically Ill Patients
04/11/112801HLA-Ig Based Artificial Antigen Presenting Cells for Efficient ex vivo Expansion of Human CTL
04/11/112477RNAi Interference by dsRNA Injection into Drosophila Embryos
04/10/112509Mechanical Manipulation of Neurons to Control Axonal Development
04/09/112698Quantitative Assessment of Immune Cells in the Injured Spinal Cord Tissue by Flow Cytometry: a Novel Use for a Cell Purification Method
04/08/112739Measurement of Aggregate Cohesion by Tissue Surface Tensiometry
04/08/112691Elastomeric PGS Scaffolds in Arterial Tissue Engineering
04/07/112585Microarray Analysis for Saccharomyces cerevisiae
04/07/112529Collecting Variable-concentration Isothermal Titration Calorimetry Datasets in Order to Determine Binding Mechanisms
04/06/112594Time-lapse Live Imaging of Clonally Related Neural Progenitor Cells in the Developing Zebrafish Forebrain
04/06/112516Live-imaging of PKC Translocation in Sf9 Cells and in Aplysia Sensory Neurons
04/05/112726Methods for ECG Evaluation of Indicators of Cardiac Risk, and Susceptibility to Aconitine-induced Arrhythmias in Rats Following Status Epilepticus
04/05/112623In Vivo Canine Muscle Function Assay
04/04/112784Quantitative Analyses of all Influenza Type A Viral Hemagglutinins and Neuraminidases using Universal Antibodies in Simple Slot Blot Assays
04/04/112551Intravital Microscopy of the Inguinal Lymph Node
04/03/112795Evisceration of Mouse Vitreous and Retina for Proteomic Analyses
04/03/112598Derivation of Thymic Lymphoma T-cell Lines from Atm-/- and p53-/- Mice
04/01/112792A Real-time Electrical Impedance Based Technique to Measure Invasion of Endothelial Cell Monolayer by Cancer Cells
04/01/112579Monitoring Dynamic Changes In Mitochondrial Calcium Levels During Apoptosis Using A Genetically Encoded Calcium Sensor
03/31/112561Isolation and Culture of Adult Epithelial Stem Cells from Human Skin
03/31/112533Using Whole Mount in situ Hybridization to Link Molecular and Organismal Biology
03/30/112503Imaging Cell Shape Change in Living Drosophila Embryos
03/30/112452Mitochondrial Isolation from Skeletal Muscle
03/29/112489Electrophysiological Measurements from a Moth Olfactory System
03/29/112309Organotypic Slice Culture of GFP-expressing Mouse Embryos for Real-time Imaging of Peripheral Nerve Outgrowth
03/28/112574Single Particle Electron Microscopy Reconstruction of the Exosome Complex Using the Random Conical Tilt Method
03/28/112501Crystallization of Membrane Proteins in Lipidic Mesophases
03/27/112633Generation of Composite Plants in Medicago truncatula used for Nodulation Assays
03/26/112763 DNA Extraction from Paraffin Embedded Material for Genetic and Epigenetic Analyses
03/25/112548Studying the Integration of Adult-born Neurons
03/25/112530VisualEyes: A Modular Software System for Oculomotor Experimentation
03/24/112625Imaging C. elegans Embryos using an Epifluorescent Microscope and Open Source Software
03/24/112557Assessing Signaling Properties of Ectodermal Epithelia During Craniofacial Development
03/23/112330Preparation of Acute Hippocampal Slices from Rats and Transgenic Mice for the Study of Synaptic Alterations during Aging and Amyloid Pathology
03/23/112567Optical Imaging of Neurons in the Crab Stomatogastric Ganglion with Voltage-sensitive Dyes
03/22/112538High-Efficiency Transduction of Liver Cancer Cells by Recombinant Adeno-Associated Virus Serotype 3 Vectors
03/22/112510Yeast Colony Embedding Method
03/21/112527Preparation and Culture of Chicken Auditory Brainstem Slices
03/21/112693Invasion of Human Cells by a Bacterial Pathogen
03/20/112655Organotypic Culture of Full-thickness Adult Porcine Retina
03/19/112624Demonstrating the Uses of the Novel Gravitational Force Spectrometer to Stretch and Measure Fibrous Proteins
03/18/112496Measuring Caenorhabditis elegans Life Span in 96 Well Microtiter Plates
03/18/112518Arabidopsis thaliana Polar Glycerolipid Profiling by Thin Layer Chromatography (TLC) Coupled with Gas-Liquid Chromatography (GLC)
03/17/112451Combining Lipophilic dye, in situ Hybridization, Immunohistochemistry, and Histology
03/17/112409Labeling F-actin Barbed Ends with Rhodamine-actin in Permeabilized Neuronal Growth Cones
03/16/112476Purification of Progenitors from Skeletal Muscle
03/16/112505Mutagenesis and Functional Selection Protocols for Directed Evolution of Proteins in E. coli
03/15/112695A Method for Ovarian Follicle Encapsulation and Culture in a Proteolytically Degradable 3 Dimensional System
03/15/112546DNA Microarrays: Sample Quality Control, Array Hybridization and Scanning
03/14/112673Induction of Alloantigen-specific Anergy in Human Peripheral Blood Mononuclear Cells by Alloantigen Stimulation with Co-stimulatory Signal Blockade
03/14/112668Preparation and Use of HIV-1 Infected Primary CD4+ T-Cells as Target Cells in Natural Killer Cell Cytotoxic Assays
03/13/112573Clonogenic Assay: Adherent Cells
03/13/112791A Noninvasive Hair Sampling Technique to Obtain High Quality DNA from Elusive Small Mammals
03/12/112368Single Port Donor Nephrectomy
03/11/112504Assaying Locomotor, Learning, and Memory Deficits in Drosophila Models of Neurodegeneration
03/11/112490C. elegans Positive Butanone Learning, Short-term, and Long-term Associative Memory Assays
03/11/112488Heterokaryon Technique for Analysis of Cell Type-specific Localization
03/10/112376Assessment of Motor Balance and Coordination in Mice using the Balance Beam
03/10/112467An Alternant Method to the Traditional NASA Hindlimb Unloading Model in Mice
03/10/111677An Experimental Platform to Study the Closed-loop Performance of Brain-machine Interfaces
03/08/112651Procedure for Lung Engineering
03/07/112596Thermal Ablation for the Treatment of Abdominal Tumors
03/07/112558Orthotopic Xenografting of Human Luciferase-Tagged Malignant Peripheral Nerve Sheath Tumor Cells for in vivo Testing of Candidate Therapeutic Agents
03/06/112639Neural-Colony Forming Cell Assay: An Assay To Discriminate Bona Fide Neural Stem Cells from Neural Progenitor Cells
03/05/112366Rapid PCR Thermocycling using Microscale Thermal Convection
03/04/112471Computer-assisted Large-scale Visualization and Quantification of Pancreatic Islet Mass, Size Distribution and Architecture
03/03/112631Detection of Signaling Effector-Complexes Downstream of BMP4 Using in situ PLA, a Proximity Ligation Assay
03/02/112065Combining Behavioral Endocrinology and Experimental Economics: Testosterone and Social Decision Making
03/02/112003Patterned Photostimulation with Digital Micromirror Devices to Investigate Dendritic Integration Across Branch Points
03/01/112662Accurate and Simple Measurement of the Pro-inflammatory Cytokine IL-1β using a Whole Blood Stimulation Assay
03/01/112619Competitive Homing Assays to Study Gut-tropic T Cell Migration
02/28/112431Respirometric Oxidative Phosphorylation Assessment in Saponin-permeabilized Cardiac Fibers
02/28/112215Primary Cell Cultures from Drosophila Gastrula Embryos
02/27/112563Dissection of a Mouse Eye for a Whole Mount of the Retinal Pigment Epithelium
02/27/112288Stable Isotopic Profiling of Intermediary Metabolic Flux in Developing and Adult Stage Caenorhabditis elegans
02/27/112497Multiple-mouse Neuroanatomical Magnetic Resonance Imaging
02/25/112473Assessment of Social Interaction Behaviors
02/25/112498Isolation of Translating Ribosomes Containing Peptidyl-tRNAs for Functional and Structural Analyses
02/24/112164Blue Native Polyacrylamide Gel Electrophoresis (BN-PAGE) for Analysis of Multiprotein Complexes from Cellular Lysates
02/24/112595Finger-stick Blood Sampling Methodology for the Determination of Exercise-induced Lymphocyte Apoptosis
02/23/112570FISH for Pre-implantation Genetic Diagnosis
02/23/112405Assaying Surface Expression of Chemosensory Receptors in Heterologous Cells
02/22/112582Myo-mechanical Analysis of Isolated Skeletal Muscle
02/22/112138Kinematics and Ground Reaction Force Determination: A Demonstration Quantifying Locomotor Abilities of Young Adult, Middle-aged, and Geriatric Rats
02/21/112422A Protocol for Comprehensive Assessment of Bulbar Dysfunction in Amyotrophic Lateral Sclerosis (ALS)
02/21/112513Isolation of Mouse Respiratory Epithelial Cells and Exposure to Experimental Cigarette Smoke at Air Liquid Interface
02/20/112580Spinal Cord Electrophysiology II: Extracellular Suction Electrode Fabrication
02/19/112541A Simple Way to Measure Ethanol Sensitivity in Flies
02/18/112547Using Luciferase to Image Bacterial Infections in Mice
02/18/112371Recombinant Retroviral Production and Infection of B Cells
02/17/112699Concentration Determination of Nucleic Acids and Proteins Using the Micro-volume Bio-spec Nano Spectrophotometer
02/17/112468Identifying the Effects of BRCA1 Mutations on Homologous Recombination using Cells that Express Endogenous Wild-type BRCA1
02/16/112464Acute Myocardial Infarction in Rats
02/16/112342Using Visual and Narrative Methods to Achieve Fair Process in Clinical Care
02/15/112439Slice Preparation, Organotypic Tissue Culturing and Luciferase Recording of Clock Gene Activity in the Suprachiasmatic Nucleus
02/15/112389The Culture of Primary Motor and Sensory Neurons in Defined Media on Electrospun Poly-L-lactide Nanofiber Scaffolds
02/14/112544Methods for Rapid Transfer and Localization of Lyme Disease Pathogens Within the Tick Gut
02/14/112532Passive Administration of Monoclonal Antibodies Against H. capsulatum and Others Fungal Pathogens
02/13/112761Mouse Model of Middle Cerebral Artery Occlusion
02/13/112640Studying Cell Rolling Trajectories on Asymmetric Receptor Patterns
02/12/112356Magnetic Resonance Derived Myocardial Strain Assessment Using Feature Tracking
02/11/112636Fabrication of Micropatterned Hydrogels for Neural Culture Systems using Dynamic Mask Projection Photolithography
02/10/112616A 1.5 Hour Procedure for Identification of Enterococcus Species Directly from Blood Cultures
02/10/112443Dorsal Column Steerability with Dual Parallel Leads using Dedicated Power Sources: A Computational Model
02/10/112442Postsynaptic Recordings at Afferent Dendrites Contacting Cochlear Inner Hair Cells: Monitoring Multivesicular Release at a Ribbon Synapse
02/09/112528Subcutaneous Infection of Methicillin Resistant Staphylococcus Aureus (MRSA)
02/09/112374Automated Interactive Video Playback for Studies of Animal Communication
02/08/112484Isolation of Mouse Salivary Gland Stem Cells
02/08/112361Recapitulation of an Ion Channel IV Curve Using Frequency Components
02/07/112432Hyperinsulinemic-Euglycemic Clamp in the Conscious Rat
02/06/112535An Orthotopic Model of Murine Bladder Cancer
02/05/111978Focal Cerebral Ischemia Model by Endovascular Suture Occlusion of the Middle Cerebral Artery in the Rat
02/04/112545Separating Beads and Cells in Multi-channel Microfluidic Devices Using Dielectrophoresis and Laminar Flow
02/04/112463Measuring Circadian and Acute Light Responses in Mice using Wheel Running Activity
02/04/112394Decellularization and Recellularization of Whole Livers
02/03/112493Developing Custom Chinese Hamster Ovary-host Cell Protein Assays using Acoustic Membrane Microparticle Technology
02/03/112462Organotypic Hippocampal Slice Cultures
02/03/112283Agar-Block Microcosms for Controlled Plant Tissue Decomposition by Aerobic Fungi
02/02/112540Expansion, Purification, and Functional Assessment of Human Peripheral Blood NK Cells
02/02/112348Isolation of Brain and Spinal Cord Mononuclear Cells Using Percoll Gradients
02/01/112661High Sensitivity 5-hydroxymethylcytosine Detection in Balb/C Brain Tissue
02/01/112461A High Throughput Screen for Biomining Cellulase Activity from Metagenomic Libraries
01/31/112336Isolation and In vitro Activation of Caenorhabditis elegans Sperm
01/30/112437Microtiter Dish Biofilm Formation Assay
01/29/112508Expired CO2 Measurement in Intubated or Spontaneously Breathing Patients from the Emergency Department
01/28/112327Determination of DNA Methylation of Imprinted Genes in Arabidopsis Endosperm
01/27/112377Contrast Enhanced Vessel Imaging using MicroCT
01/27/112303A Mouse Model of in Utero Transplantation
01/26/112352In vivo and in vitro Studies of Adaptor-clathrin Interaction
01/26/112367An Isolated Retinal Preparation to Record Light Response from Genetically Labeled Retinal Ganglion Cells
01/26/112332Environmentally Induced Heritable Changes in Flax
01/26/111959Examination of the Telomere G-overhang Structure in Trypanosoma brucei
01/25/112512An Analytical Tool-box for Comprehensive Biochemical, Structural and Transcriptome Evaluation of Oral Biofilms Mediated by Mutans Streptococci
01/24/112373Nucleofection and Primary Culture of Embryonic Mouse Hippocampal and Cortical Neurons
01/24/112282Behavioural Pharmacology in Classical Conditioning of the Proboscis Extension Response in Honeybees (Apis mellifera)
01/23/112455Dissection of Human Vitreous Body Elements for Proteomic Analysis
01/22/112414Generation of Comprehensive Thoracic Oncology Database - Tool for Translational Research
01/21/112517A Microfluidic-based Hydrodynamic Trap for Single Particles
01/21/112494Electrospinning Fundamentals: Optimizing Solution and Apparatus Parameters
01/20/112474RNA Interference in Ticks
01/20/112481In vitro Biofilm Formation in an 8-well Chamber Slide
01/19/112314Optimized Transfection Strategy for Expression and Electrophysiological Recording of Recombinant Voltage-Gated Ion Channels in HEK-293T Cells
01/19/112247In vivo Quantification of G Protein Coupled Receptor Interactions using Spectrally Resolved Two-photon Microscopy
01/18/112325Modeling Biological Membranes with Circuit Boards and Measuring Electrical Signals in Axons: Student Laboratory Exercises
01/18/112324Physiological Experimentation with the Crayfish Hindgut: A Student Laboratory Exercise
01/18/112322Membrane Potentials, Synaptic Responses, Neuronal Circuitry, Neuromodulation and Muscle Histology Using the Crayfish: Student Laboratory Exercises
01/17/112364Guidelines for Elective Pediatric Fiberoptic Intubation
01/17/111421A Novel Rescue Technique for Difficult Intubation and Difficult Ventilation
01/16/112317A Novel Technique of Rescuing Capsulorhexis Radial Tear-out using a Cystotome
01/16/112285Protein Crystallization for X-ray Crystallography
01/15/112383Pseudomonas aeruginosa and Saccharomyces cerevisiae Biofilm in Flow Cells
01/14/112381Ex vivo Expansion of Tumor-reactive T Cells by Means of Bryostatin 1/Ionomycin and the Common Gamma Chain Cytokines Formulation
01/14/112412Electrophysiological Recordings from the Giant Fiber Pathway of D. melanogaster
01/13/112214Analysis of Schwann-astrocyte Interactions Using In Vitro Assays
01/12/112226An Investigation of the Effects of Sports-related Concussion in Youth Using Functional Magnetic Resonance Imaging and the Head Impact Telemetry System
01/11/112382Microdissection of Black Widow Spider Silk-producing Glands
01/11/112331The Production of C. elegans Transgenes via Recombineering with the galK Selectable Marker
01/11/112306High-resolution Fiber-optic Microendoscopy for in situ Cellular Imaging
01/11/112293An Introduction to Worm Lab: from Culturing Worms to Mutagenesis
01/11/112562High-density EEG Recordings of the Freely Moving Mice using Polyimide-based Microelectrode
01/11/112457Establishing Embryonic Mouse Neural Stem Cell Culture Using the Neurosphere Assay
01/10/112269Patch Clamp and Perfusion Techniques for Studying Ion Channels Expressed in Xenopus oocytes
01/07/112466Direct Delivery of MIF Morpholinos Into the Zebrafish Otocyst by Injection and Electroporation Affects Inner Ear Development
01/07/112199Growth and Differentiation of Adult Hippocampal Arctic Ground Squirrel Neural Stem Cells
01/06/112586Principles of Rodent Surgery for the New Surgeon
01/06/112265A Procedure for Studying the Footshock-Induced Reinstatement of Cocaine Seeking in Laboratory Rats
01/06/112423Modeling Stroke in Mice - Middle Cerebral Artery Occlusion with the Filament Model
01/06/112156Intra-Operative Behavioral Tasks in Awake Humans Undergoing Deep Brain Stimulation Surgery
01/05/112407Streamlined Purification of Plasmid DNA From Prokaryotic Cultures
01/04/112358Measurement of Bioelectric Current with a Vibrating Probe
01/04/112084Tri-layered Electrospinning to Mimic Native Arterial Architecture using Polycaprolactone, Elastin, and Collagen: A Preliminary Study
01/03/112365Protocol for Production of a Genetic Cross of the Rodent Malaria Parasites
01/03/112194Corneal Confocal Microscopy: A Novel Non-invasive Technique to Quantify Small Fibre Pathology in Peripheral Neuropathies
01/02/112397Facilitating the Analysis of Immunological Data with Visual Analytic Techniques
12/30/102243Large Scale Zebrafish-Based In vivo Small Molecule Screen
12/30/102129Bioassays for Monitoring Insecticide Resistance
12/29/102190Testing Protozoacidal Activity of Ligand-lytic Peptides Against Termite Gut Protozoa in vitro (Protozoa Culture) and in vivo (Microinjection into Termite Hindgut)
12/29/102158Isolation of Valvular Endothelial Cells
12/28/102273State-Dependency Effects on TMS: A Look at Motive Phosphene Behavior
12/28/102272TMS: Using the Theta-Burst Protocol to Explore Mechasnism of Plasticity in Individuals with Fragile X Syndrome and Autism
12/28/102271Combining Transcranial Magnetic Stimulation and fMRI to Examine the Default Mode Network
12/27/102531Genotypic Inference of HIV-1 Tropism Using Population-based Sequencing of V3
12/27/102438Paraffin-Embedded and Frozen Sections of Drosophila Adult Muscles
12/27/102470The Use of Drip Flow and Rotating Disk Reactors for Staphylococcus aureus Biofilm Analysis
12/26/102224Microfabricated Platforms for Mechanically Dynamic Cell Culture
12/26/102355Transfection and Mutagenesis of Target Genes in Mosquito Cells by Locked Nucleic Acid-modified Oligonucleotides
12/24/101902Two-Photon-Based Photoactivation in Live Zebrafish Embryos
12/23/102315Real-time Imaging of Leukotriene B4 Mediated Cell Migration and BLT1 Interactions with β-arrestin
12/22/102413Micropatterned Surfaces to Study Hyaluronic Acid Interactions with Cancer Cells
12/22/102055Dechorionation of Medaka Embryos and Cell Transplantation for the Generation of Chimeras
12/22/101937Microinjection of Medaka Embryos for use as a Model Genetic Organism
12/21/102220Visualization of UV-induced Replication Intermediates in E. coli using Two-dimensional Agarose-gel Analysis
12/21/102275Application of a NMDA Receptor Conductance in Rat Midbrain Dopaminergic Neurons Using the Dynamic Clamp Technique
12/21/102346Whole-Cell Recording of Calcium Release-Activated Calcium (CRAC) Currents in Human T Lymphocytes
12/20/102378Ex Vivo Culture of Patient Tissue & Examination of Gene Delivery
12/20/102264Profiling of Methyltransferases and Other S-adenosyl-L-homocysteine-binding Proteins by Capture Compound Mass Spectrometry (CCMS)
12/20/102109Fabrication of Biologically Derived Injectable Materials for Myocardial Tissue Engineering
12/19/102483Localizing Protein in 3D Neural Stem Cell Culture: a Hybrid Visualization Methodology
12/19/102094An Assay for Measuring the Activity of Escherichia coli Inducible Lysine Decarboxyase
12/18/102299Colon Ascendens Stent Peritonitis (CASP) - a Standardized Model for Polymicrobial Abdominal Sepsis
12/18/102195Colony Forming Cell (CFC) Assay for Human Hematopoietic Cells
12/17/102182Induction of Experimental Autoimmune Hypophysitis in SJL Mice
12/17/102181Preparation of Mouse Pituitary Immunogen for the Induction of Experimental Autoimmune Hypophysitis
12/16/102320Combining Computer Game-Based Behavioural Experiments With High-Density EEG and Infrared Gaze Tracking
12/16/102246A Novel Method for Assessing Proximal and Distal Forelimb Function in the Rat: the Irvine, Beatties and Bresnahan (IBB) Forelimb Scale
12/15/102145Contrast Ultrasound Targeted Treatment of Gliomas in Mice via Drug-Bearing Nanoparticle Delivery and Microvascular Ablation
12/15/102492One Dimensional Turing-Like Handshake Test for Motor Intelligence
12/14/102316Isolation and Culture of Pulmonary Endothelial Cells from Neonatal Mice
12/14/102173Preparation of Complaint Matrices for Quantifying Cellular Contraction
12/13/102177Fabrication of Micro-tissues using Modules of Collagen Gel Containing Cells
12/12/102410Development of a Negative Selectable Marker for Entamoeba histolytica
12/11/102486An Organotypic Slice Assay for High-Resolution Time-Lapse Imaging of Neuronal Migration in the Postnatal Brain
12/11/102150Visualization of the Embryonic Nervous System in Whole-mount Drosophila Embryos
12/10/102362Peptides from Phage Display Library Modulate Gene Expression in Mesenchymal Cells and Potentiate Osteogenesis in Unicortical Bone Defects
12/09/102459Registered Bioimaging of Nanomaterials for Diagnostic and Therapeutic Monitoring
12/09/102460Methods Development for Blood Borne Macrophage Carriage of Nanoformulated Antiretroviral Drugs
12/08/102095Dual-mode Imaging of Cutaneous Tissue Oxygenation and Vascular Function
12/08/102344Analysis of Pluripotent Stem Cells by using Cryosections of Embryoid Bodies
12/07/102166Immunofluorescent Detection of Two Thymidine Analogues (CldU and IdU) in Primary Tissue
12/06/102511Bioenergetic Profile Experiment using C2C12 Myoblast Cells
12/05/102062Non-invasive Imaging of Leukocyte Homing and Migration in vivo
12/04/101989Orthotopic Aortic Transplantation: A Rat Model to Study the Development of Chronic Vasculopathy
12/04/102387Analysis of mRNA Nuclear Export Kinetics in Mammalian Cells by Microinjection
12/03/102210Profiling of Pre-micro RNAs and microRNAs using Quantitative Real-time PCR (qPCR) Arrays
12/03/102134Expression, Detergent Solubilization, and Purification of a Membrane Transporter, the MexB Multidrug Resistance Protein
12/02/102514Improved Visualization and Quantitative Analysis of Drug Effects Using Micropatterned Cells
12/02/102066IP-FCM: Immunoprecipitation Detected by Flow Cytometry
12/01/102435Gastrointestinal Motility Monitor (GIMM)
12/01/102082Targeting of Deep Brain Structures with Microinjections for Delivery of Drugs, Viral Vectors, or Cell Transplants
11/30/102213Surgical Management of Meatal Stenosis with Meatoplasty
11/29/102499Closed System Cell Culture Protocol Using HYPERStack Vessels with Gas Permeable Material Technology
11/28/102146An Orthotopic Model of Serous Ovarian Cancer in Immunocompetent Mice for in vivo Tumor Imaging and Monitoring of Tumor Immune Responses
11/27/102380Production of Chick Embryo Extract for the Cultivation of Murine Neural Crest Stem Cells
11/26/102204Determining Cell Number During Cell Culture using the Scepter Cell Counter
11/26/102077Experimental Metastasis and CTL Adoptive Transfer Immunotherapy Mouse Model
11/24/102152Generation of RNA/DNA Hybrids in Genomic DNA by Transformation using RNA-containing Oligonucleotides
11/24/102363Alphavirus Transducing System: Tools for Visualizing Infection in Mosquito Vectors
11/23/102221Enzyme-linked Immunospot Assay (ELISPOT): Quantification of Th-1 Cellular Immune Responses Against Microbial Antigens
11/22/102565NanoDrop Microvolume Quantitation of Nucleic Acids
11/21/101712Crystallizing Membrane Proteins for Structure Determination using Lipidic Mesophases
11/20/102351Paired Patch Clamp Recordings from Motor-neuron and Target Skeletal Muscle in Zebrafish
11/20/102393Isolation and Expansion of the Adult Mouse Neural Stem Cells Using the Neurosphere Assay
11/19/102349Monitoring Immune Cells Trafficking Fluorescent Prion Rods Hours after Intraperitoneal Infection
11/18/102184Intravital Microscopy of the Mouse Brain Microcirculation using a Closed Cranial Window
11/18/102323Muscle Receptor Organs in the Crayfish Abdomen: A Student Laboratory Exercise in Proprioception
11/17/102140Intracranial Injection of Adeno-associated Viral Vectors
11/17/102319Physiological Recordings of High and Low Output NMJs on the Crayfish Leg Extensor Muscle
11/16/102270Quantifying Synapses: an Immunocytochemistry-based Assay to Quantify Synapse Number
11/15/102147Visualization of Mitochondrial DNA Replication in Individual Cells by EdU Signal Amplification
11/14/102354Automated Sholl Analysis of Digitized Neuronal Morphology at Multiple Scales
11/13/102047Ultrasound-Guided Microinjection into the Mouse Forebrain In Utero at E9.5
11/12/102345The NeuroStar TMS Device: Conducting the FDA Approved Protocol for Treatment of Depression
11/11/102384Cryopreservation of Cortical Tissue Blocks for the Generation of Highly Enriched Neuronal Cultures
11/11/102004How to Build a Laser Speckle Contrast Imaging (LSCI) System to Monitor Blood Flow
11/10/102030How to Create and Use Binocular Rivalry
11/10/102292Operant Sensation Seeking in the Mouse
11/09/102250Antigen Specific In Vivo Killing Assay using CFSE Labeled Target Cells
11/08/102445Detection of Functional Matrix Metalloproteinases by Zymography
11/07/102429Quantitative Measurement of GLUT4 Translocation to the Plasma Membrane by Flow Cytometry
11/06/102032 Experimental Models for Study of Retinal Pigment Epithelial Physiology and Pathophysiology
11/05/102465Quantification of dsDNA using the Hitachi F-7000 Fluorescence Spectrophotometer and PicoGreen Dye
11/04/102428Bronchial Thermoplasty: A Novel Therapeutic Approach to Severe Asthma
11/04/102223The C-seal: A Biofragmentable Drain Protecting the Stapled Colorectal Anastomosis from Leakage
11/03/102006Cargo Loading onto Kinesin Powered Molecular Shuttles
11/03/102279An Allele-specific Gene Expression Assay to Test the Functional Basis of Genetic Associations
11/02/101864Fertilization of Xenopus oocytes using the Host Transfer Method
11/01/102064Label-free in situ Imaging of Lignification in Plant Cell Walls
10/31/102312Comprehensive & Cost Effective Laboratory Monitoring of HIV/AIDS: an African Role Model
10/30/101747Performing and Processing FNA of Anterior Fat Pad for Amyloid
10/29/101846Assessing Two-dimensional Crystallization Trials of Small Membrane Proteins for Structural Biology Studies by Electron Crystallography
10/28/102106The Organotypic Hippocampal Slice Culture Model for Examining Neuronal Injury
10/27/102159Isolation and Culture of Avian Embryonic Valvular Progenitor Cells
10/26/102123Oral Administration of Rotenone using a Gavage and Image Analysis of Alpha-synuclein Inclusions in the Enteric Nervous System
10/25/102060Chronic Imaging of Mouse Visual Cortex Using a Thinned-skull Preparation
10/24/102045Visualisation and Quantification of Intracellular Interactions of Neisseria meningitidis and Human α-actinin by Confocal Imaging
10/23/102154An ex-ovo Chicken Embryo Culture System Suitable for Imaging and Microsurgery Applications
10/22/102307Retroviral Transduction of T-cell Receptors in Mouse T-cells
10/21/102287A 96 Well Microtiter Plate-based Method for Monitoring Formation and Antifungal Susceptibility Testing of Candida albicans Biofilms
10/20/102225Multispectral Real-time Fluorescence Imaging for Intraoperative Detection of the Sentinel Lymph Node in Gynecologic Oncology
10/19/102390In vitro tRNA Methylation Assay with the Entamoeba histolytica DNA and tRNA Methyltransferase Dnmt2 (Ehmeth) Enzyme
10/18/102308Combination of Adhesive-tape-based Sampling and Fluorescence in situ Hybridization for Rapid Detection of Salmonella on Fresh Produce
10/17/102085Differential Imaging of Biological Structures with Doubly-resonant Coherent Anti-stokes Raman Scattering (CARS)
10/16/102260Changes in Mammary Gland Morphology and Breast Cancer Risk in Rats
10/15/102167Assessing Endothelial Vasodilator Function with the Endo-PAT 2000
10/15/102151In vivo Visualization of Synaptic Vesicles Within Drosophila Larval Segmental Axons
10/14/102359Western Blotting: Sample Preparation to Detection
10/13/102155Design and Construction of a Cost Effective Headstage for Simultaneous Neural Stimulation and Recording in the Water Maze
10/13/102131Gramicidin-based Fluorescence Assay; for Determining Small Molecules Potential for Modifying Lipid Bilayer Properties
10/12/102259The Use of Carboxyfluorescein Diacetate Succinimidyl Ester (CFSE) to Monitor Lymphocyte Proliferation
10/11/102139Making Sense of Listening: The IMAP Test Battery
10/10/102149Imaging Protein-protein Interactions in vivo
10/09/102252Assaying β-amyloid Toxicity using a Transgenic C. elegans Model
10/08/102103In utero Electroporation followed by Primary Neuronal Culture for Studying Gene Function in Subset of Cortical Neurons
10/07/102061Visualizing Cell-to-cell Transfer of HIV using Fluorescent Clones of HIV and Live Confocal Microscopy
10/06/102186Co-culture Models of Pseudomonas aeruginosa Biofilms Grown on Live Human Airway Cells
10/05/102033Establishing Primary Adult Fibroblast Cultures From Rodents
10/04/102072Live Cell Response to Mechanical Stimulation Studied by Integrated Optical and Atomic Force Microscopy
10/03/102153Intracranial Orthotopic Allografting of Medulloblastoma Cells in Immunocompromised Mice
10/02/102185Assessing Stomatal Response to Live Bacterial Cells using Whole Leaf Imaging
10/01/102189Mutagenesis and Functional Analysis of Ion Channels Heterologously Expressed in Mammalian Cells
09/30/102168Transnuclear Mice with Pre-defined T Cell Receptor Specificities Against Toxoplasma gondii Obtained Via SCNT
09/29/102128Visualization of Larval Segmental Nerves in 3rd Instar Drosophila Larval Preparations
09/28/102157Assaying Locomotor Activity to Study Circadian Rhythms and Sleep Parameters in Drosophila
09/27/102161Assessment of Cerebral Lateralization in Children using Functional Transcranial Doppler Ultrasound (fTCD)
09/26/102169Isolation of Stem Cells from Human Pancreatic Cancer Xenografts
09/25/102133Single Cell Transfection in Chick Embryos
09/24/102087Axoplasm Isolation from Rat Sciatic Nerve
09/23/102198Specific Marking of HIV-1 Positive Cells using a Rev-dependent Lentiviral Vector Expressing the Green Fluorescent Protein
09/23/102202Preparation of Highly Coupled Rat Heart Mitochondria
09/22/102196Estimating Virus Production Rates in Aquatic Systems
09/21/102051Adult and Embryonic Skeletal Muscle Microexplant Culture and Isolation of Skeletal Muscle Stem Cells
09/20/102093Multicolor Time-lapse Imaging of Transgenic Zebrafish: Visualizing Retinal Stem Cells Activated by Targeted Neuronal Cell Ablation
09/19/102059A Thin-skull Window Technique for Chronic Two-photon In vivo Imaging of Murine Microglia in Models of Neuroinflammation
09/18/102201Technique to Collect Fungiform (Taste) Papillae from Human Tongue
09/17/102244Gene Delivery to Postnatal Rat Brain by Non-ventricular Plasmid Injection and Electroporation
09/16/102026Comparative in vivo Study of gp96 Adjuvanticity in the Frog Xenopus laevis
09/15/102118Assessment of Immunologically Relevant Dynamic Tertiary Structural Features of the HIV-1 V3 Loop Crown R2 Sequence by ab initio Folding
09/14/102078Identifying Dysregulated Genes Induced by Kaposi's Sarcoma-associated Herpesvirus (KSHV)
09/13/102175Upright Imaging of Drosophila Embryos
09/12/102107Patch Clamp Recordings from Mouse Retinal Neurons in a Dark-adapted Slice Preparation
09/11/102209Isolation of Retinal Stem Cells from the Mouse Eye
09/10/102249In vivo Imaging of Intact Drosophila Larvae at Sub-cellular Resolution
09/09/102160Adenovirus-mediated Genetic Removal of Signaling Molecules in Cultured Primary Mouse Embryonic Fibroblasts
09/08/102002Analysis of DNA Double-strand Break (DSB) Repair in Mammalian Cells
09/07/102142Detection of Neu1 Sialidase Activity in Regulating TOLL-like Receptor Activation
09/06/102111Study Glial Cell Heterogeneity Influence on Axon Growth Using a New Coculture Method
09/05/102136Electroeluting DNA Fragments
09/04/102144Direct Analysis of Single Cells by Mass Spectrometry at Atmospheric Pressure
09/03/102097Atmospheric-pressure Molecular Imaging of Biological Tissues and Biofilms by LAESI Mass Spectrometry
09/02/102192Strategies for Study of Neuroprotection from Cold-preconditioning
09/01/102086Isolation, Enrichment, and Maintenance of Medulloblastoma Stem Cells
08/31/102069Assessment of Cardiac Function and Energetics in Isolated Mouse Hearts Using 31P NMR Spectroscopy
08/30/102126Intraperitoneal Injection into Adult Zebrafish
08/29/101941Direct Tracheal Instillation of Solutes into Mouse Lung
08/28/102170The Method of Rodent Whole Embryo Culture using the Rotator-type Bottle Culture System
08/27/102208A Cell-to-cell Macromolecular Transport Assay in Planta Utilizing Biolistic Bombardment
08/26/102122Electron Spin Resonance Micro-imaging of Live Species for Oxygen Mapping
08/25/102137RNAi Screening for Host Factors Involved in Vaccinia Virus Infection using Drosophila Cells
08/24/101942Experimental Metastasis Assay
08/23/102090Generation of Transgenic C. elegans by Biolistic Transformation
08/22/102083RhoC GTPase Activation Assay
08/21/102010Production of Transgenic Xenopus laevis by Restriction Enzyme Mediated Integration and Nuclear Transplantation
08/20/101998Novel Apparatus and Method for Drug Reinforcement
08/19/101901Simultaneous fMRI and Electrophysiology in the Rodent Brain
08/18/102099Bacterial Delivery of RNAi Effectors: Transkingdom RNAi
08/17/102125In vivo Imaging and Therapeutic Treatments in an Orthotopic Mouse Model of Ovarian Cancer
08/16/101992Systemic and Local Drug Delivery for Treating Diseases of the Central Nervous System in Rodent Models
08/15/102108Eye Movement Monitoring of Memory
08/14/102102Double Fluorescence in situ Hybridization in Fresh Brain Sections
08/13/102130Induction and Assessment of Class Switch Recombination in Purified Murine B Cells
08/12/102091Toxoplasma gondii Cyst Wall Formation in Activated Bone Marrow-derived Macrophages and Bradyzoite Conditions
08/11/102020A Reverse Genetic Approach to Test Functional Redundancy During Embryogenesis
08/10/102089Studying the Effects of Matrix Stiffness on Cellular Function using Acrylamide-based Hydrogels
08/09/101993Human Fear Conditioning Conducted in Full Immersion 3-Dimensional Virtual Reality
08/08/102100Murine Echocardiography and Ultrasound Imaging
08/07/102203Evaluation of the Spatial Distribution of γH2AX following Ionizing Radiation
08/06/102038Recording Multicellular Behavior in Myxococcus xanthus Biofilms using Time-lapse Microcinematography
08/05/102070Transurethral Induction of Mouse Urinary Tract Infection
08/04/102079Intravenous Microinjections of Zebrafish Larvae to Study Acute Kidney Injury
08/03/102057Generation of Recombinant Influenza Virus from Plasmid DNA
08/02/102027DNA Stable-Isotope Probing (DNA-SIP)
08/01/102036Fate Mapping of Human Embryonic Stem Cells by Teratoma Formation
07/31/101985T-wave Ion Mobility-mass Spectrometry: Basic Experimental Procedures for Protein Complex Analysis
07/30/102176Preparation of Rat Tail Tendons for Biomechanical and Mechanobiological Studies
07/29/102053Chromatin Immunoprecipitation (ChIP) to Assay Dynamic Histone Modification in Activated Gene Expression in Human Cells
07/28/101931Deficient Pms2, ERCC1, Ku86, CcOI in Field Defects During Progression to Colon Cancer
07/27/101980In vivo Imaging of Transgenic Leishmania Parasites in a Live Host
07/26/102039Primer-Free Aptamer Selection Using A Random DNA Library
07/25/101976Labeling and Imaging Cells in the Zebrafish Hindbrain
07/24/101988Basics of Multivariate Analysis in Neuroimaging Data
07/23/101958In situ Subcellular Fractionation of Adherent and Non-adherent Mammalian Cells
07/22/102041Assessment of Ultrasonic Vocalizations During Drug Self-administration in Rats
07/21/102076The Vermicelli and Capellini Handling Tests: Simple quantitative measures of dexterous forepaw function in rats and mice
07/20/102021Preparation of Mouse Brain Tissue for Immunoelectron Microscopy
07/19/101924Transplantation of GFP-expressing Blastomeres for Live Imaging of Retinal and Brain Development in Chimeric Zebrafish Embryos
07/18/102242Dissection of Oenocytes from Adult Drosophila melanogaster
07/17/101977Murine Bioluminescent Hepatic Tumour Model
07/16/101722Mosaic Zebrafish Transgenesis for Evaluating Enhancer Sequences
07/15/102236Feeder-Free Adaptation, Culture and Passaging of Human IPS Cells using Complete KnockOut Serum Replacement Feeder-Free Medium
07/15/102237Cryopreserving and Recovering of Human iPS Cells using Complete KnockOut Serum Replacement Feeder-Free Medium
07/14/101884Preparation of Oligomeric β-amyloid1-42 and Induction of Synaptic Plasticity Impairment on Hippocampal Slices
07/13/101986Establishing Intracranial Brain Tumor Xenografts With Subsequent Analysis of Tumor Growth and Response to Therapy using Bioluminescence Imaging
07/12/102022Orthotopic Hind-Limb Transplantation in Rats
07/11/102071Xenotransplantation of Human Stem Cells into the Chicken Embryo
07/10/101546Purification of Specific Cell Population by Fluorescence Activated Cell Sorting (FACS)
07/09/101781High-frequency High-resolution Echocardiography: First Evidence on Non-invasive Repeated Measure of Myocardial Strain, Contractility, and Mitral Regurgitation in the Ischemia-reperfused Murine Heart
07/08/102050Measuring Calpain Activity in Fixed and Living Cells by Flow Cytometry
07/07/102101Genome-wide Analysis using ChIP to Identify Isoform-specific Gene Targets
07/06/102081DiOLISTIC Labeling of Neurons from Rodent and Non-human Primate Brain Slices
07/05/102011Isolating Stem Cells from Soft Musculoskeletal Tissues
07/03/101940Isolation of Human Umbilical Arterial Smooth Muscle Cells (HUASMC)
07/02/102034PAR-CliP - A Method to Identify Transcriptome-wide the Binding Sites of RNA Binding Proteins
07/01/101991Cell Electrofusion Visualized with Fluorescence Microscopy
06/30/102035Mouse Embryonic Lung Culture, A System to Evaluate the Molecular Mechanisms of Branching
06/29/102044Method for the Isolation of Francisella tularensis Outer Membranes
06/28/102063Quantitation of γH2AX Foci in Tissue Samples
06/27/102028Microdissection of Zebrafish Embryonic Eye Tissues
06/26/101995Photoconversion of Purified Fluorescent Proteins and Dual-probe Optical Highlighting in Live Cells
06/25/102014One-step Metabolomics: Carbohydrates, Organic and Amino Acids Quantified in a Single Procedure
06/24/101900Development of automated imaging and analysis for zebrafish chemical screens.
06/23/102005In-vivo Centrifugation of Drosophila Embryos
06/22/101861A Model of Disturbed Flow-Induced Atherosclerosis in Mouse Carotid Artery by Partial Ligation and a Simple Method of RNA Isolation from Carotid Endothelium
06/21/102048Using Learning Outcome Measures to assess Doctoral Nursing Education
06/20/102046OLIgo Mass Profiling (OLIMP) of Extracellular Polysaccharides
06/19/101954Analyzing Large Protein Complexes by Structural Mass Spectrometry
06/18/102007Genome-wide Analysis of Aminoacylation (Charging) Levels of tRNA Using Microarrays
06/17/101700Quantitative Real-Time PCR using the Thermo Scientific Solaris qPCR Assay
06/16/101996Techniques for Imaging Ca2+ Signaling in Human Sperm
06/15/101948Eukaryotic Polyribosome Profile Analysis
06/14/101668Functional Mapping with Simultaneous MEG and EEG
06/13/101935Rapid Homogeneous Detection of Biological Assays Using Magnetic Modulation Biosensing System
06/12/101963Bimolecular Fluorescence Complementation (BiFC) Assay for Protein-Protein Interaction in Onion Cells Using the Helios Gene Gun
06/11/101952Floral-dip Transformation of Arabidopsis thaliana to Examine pTSO2::β-glucuronidase Reporter Gene Expression
06/10/101849Mammary Epithelial Transplant Procedure
06/09/102040Single-Molecule Imaging of Nuclear Transport
06/08/101964Derivation of Mouse Trophoblast Stem Cells from Blastocysts
06/07/101970In situ Quantification of Pancreatic Beta-cell Mass in Mice
06/06/101878Time-lapse Imaging of Mitosis After siRNA Transfection
06/05/101894Vascular Occlusion Training for Inclusion Body Myositis: A Novel Therapeutic Approach
06/04/101791Electrode Fabrication and Implantation in Aplysia californica for Multi-channel Neural and Muscular Recordings in Intact, Freely Behaving Animals
06/03/101982Preparation of Drosophila S2 cells for Light Microscopy
06/02/101915Optical Scatter Microscopy Based on Two-Dimensional Gabor Filters
06/01/102017Human T Lymphocyte Isolation, Culture and Analysis of Migration In Vitro
05/31/101947Chronic Salmonella Infected Mouse Model
05/30/102056How to Culture, Record and Stimulate Neuronal Networks on Micro-electrode Arrays (MEAs)
05/29/101925Investigating Tissue- and Organ-specific Phytochrome Responses using FACS-assisted Cell-type Specific Expression Profiling in Arabidopsis thaliana
05/28/101738Transthoracic Echocardiography in Mice
05/27/101739Ambulatory ECG Recording in Mice
05/26/101730Programmed Electrical Stimulation in Mice
05/25/101773Dissection and Culture of Commissural Neurons from Embryonic Spinal Cord
05/24/102016Laser Capture Microdissection of Drosophila Peripheral Neurons
05/23/101949Live-cell Imaging and Quantitative Analysis of Embryonic Epithelial Cells in Xenopus laevis
05/22/101786Low-stress Route Learning Using the Lashley III Maze in Mice
05/21/101787A Simple Composite Phenotype Scoring System for Evaluating Mouse Models of Cerebellar Ataxia
05/20/101933Video Bioinformatics Analysis of Human Embryonic Stem Cell Colony Growth
05/19/101960Differentiation of Embryonic Stem Cells into Oligodendrocyte Precursors
05/18/101951Mouse Models of Periventricular Leukomalacia
05/17/101876Fluorescent Labeling of COS-7 Expressing SNAP-tag Fusion Proteins for Live Cell Imaging
05/16/101932Tissue Determination Using the Animal Cap Transplant (ACT) Assay in Xenopus laevis
05/15/101962A Novel Capsulorhexis Technique Using Shearing Forces with Cystotome
05/14/101956Recording Behavioral Responses to Reflection in Crayfish
05/13/101955Selection of Aptamers for Amyloid β-Protein, the Causative Agent of Alzheimer's Disease
05/12/102008MISSION esiRNA for RNAi Screening in Mammalian Cells
05/06/101869Hi-C: A Method to Study the Three-dimensional Architecture of Genomes.
05/06/101938The Subventricular Zone En-face: Wholemount Staining and Ependymal Flow
05/06/101943Electron Cryotomography of Bacterial Cells
05/06/101946Biocontained Carcass Composting for Control of Infectious Disease Outbreak in Livestock
05/05/101733Robotics and Dynamic Image Analysis for Studies of Gene Expression in Plant Tissues
05/04/101974Analysis of Gene Expression in Emerald Ash Borer (Agrilus planipennis) Using Quantitative Real Time-PCR
05/03/101741Window on a Microworld: Simple Microfluidic Systems for Studying Microbial Transport in Porous Media
05/02/101792Deciphering Axonal Pathways of Genetically Defined Groups of Neurons in the Chick Neural Tube Utilizing in ovo Electroporation
04/30/101895Laser Microdissection Applied to Gene Expression Profiling of Subset of Cells from the Drosophila Wing Disc
04/29/101919Direct Restart of a Replication Fork Stalled by a Head-On RNA Polymerase
04/28/101871DNA-based Fish Species Identification Protocol
04/27/101899Pulse-chase Analysis of N-linked Sugar Chains from Glycoproteins in Mammalian Cells
04/26/102012Measuring the Bending Stiffness of Bacterial Cells Using an Optical Trap
04/23/101916SDS-PAGE/Immunoblot Detection of Aβ Multimers in Human Cortical Tissue Homogenates using Antigen-Epitope Retrieval
04/22/101743Multicolor Flow Cytometry Analyses of Cellular Immune Response in Rhesus Macaques
04/21/101729Transverse Aortic Constriction in Mice
04/20/101749Microfluidic Co-culture of Epithelial Cells and Bacteria for Investigating Soluble Signal-mediated Interactions
04/19/101779A Microfluidic Device for Quantifying Bacterial Chemotaxis in Stable Concentration Gradients
04/16/101945High Density Event-related Potential Data Acquisition in Cognitive Neuroscience
04/15/101710Magnetic Resonance Spectroscopy of live Drosophila melanogaster using Magic Angle Spinning
04/14/101904Using an Automated Cell Counter to Simplify Gene Expression Studies: siRNA Knockdown of IL-4 Dependent Gene Expression in Namalwa Cells
04/13/101720A Reversible, Non-invasive Method for Airway Resistance Measurements and Bronchoalveolar Lavage Fluid Sampling in Mice
04/12/101918Linearization of the Bradford Protein Assay
04/09/101944Immunocytochemical Analysis of Human Pluripotent Stem Cells using a Self-Made Cytospin Apparatus
04/08/101190Monitoring Acupuncture Effects on Human Brain by fMRI
04/06/101957Quantification of γH2AX Foci in Response to Ionising Radiation
04/05/101793Implantation of Ferumoxides Labeled Human Mesenchymal Stem Cells in Cartilage Defects
04/02/101887Single-unit In vivo Recordings from the Optic Chiasm of Rat
04/01/101762A Novel Approach for Documenting Phosphenes Induced by Transcranial Magnetic Stimulation
03/31/101874Application of Stopped-flow Kinetics Methods to Investigate the Mechanism of Action of a DNA Repair Protein
03/30/101865Identification of Growth Inhibition Phenotypes Induced by Expression of Bacterial Type III Effectors in Yeast
03/29/101923Measuring the 50% Haemolytic Complement (CH50) Activity of Serum
03/26/101789Primary Human Bronchial Epithelial Cells Grown from Explants
03/25/101844Purification of the M. magneticum Strain AMB-1 Magnetosome Associated Protein MamAΔ41
03/24/101863Analysis of the Development of a Morphological Phenotype as a Function of Protein Concentration in Budding Yeast
03/23/101689Direct Observation of Enzymes Replicating DNA Using a Single-molecule DNA Stretching Assay
03/22/101852Isolation and Enrichment of Rat Mesenchymal Stem Cells (MSCs) and Separation of Single-colony Derived MSCs
03/19/101969In-vivo Detection of Protein-protein Interactions on Micro-patterned Surfaces
03/18/101639Genetic Studies of Human DNA Repair Proteins Using Yeast as a Model System
03/17/101746The Importance of Correct Protein Concentration for Kinetics and Affinity Determination in Structure-function Analysis
03/16/101856Tomato Analyzer: A Useful Software Application to Collect Accurate and Detailed Morphological and Colorimetric Data from Two-dimensional Objects
03/15/101936Preparation of Developing and Adult Drosophila Brains and Retinae for Live Imaging
03/12/101837Comprehensive Compositional Analysis of Plant Cell Walls (Lignocellulosic biomass) Part II: Carbohydrates
03/11/101745Comprehensive Compositional Analysis of Plant Cell Walls (Lignocellulosic biomass) Part I: Lignin
03/09/101855Isolation of Labile Multi-protein Complexes by in vivo Controlled Cellular Cross-Linking and Immuno-magnetic Affinity Chromatography
03/08/101853Assaying the Ability of Diffusible Signaling Molecules to Reorient Embryonic Spinal Commissural Axons
03/05/101734Murine Model of CD40-activation of B cells
03/04/101717Dissection of Organs from the Adult Zebrafish
03/03/101731Synthesis and Calibration of Phosphorescent Nanoprobes for Oxygen Imaging in Biological Systems
03/02/101850Analyzing Responses of Mouse Olfactory Sensory Neurons Using the Air-phase Electroolfactogram Recording
03/01/101845Multielectrode Array Recordings of the Vomeronasal Epithelium
02/26/101636Measuring Diffusion Coefficients via Two-photon Fluorescence Recovery After Photobleaching
02/25/101862Expression of Recombinant Proteins in the Methylotrophic Yeast Pichia pastoris
02/24/101724Neutrophil Extracellular Traps: How to Generate and Visualize Them
02/23/101678Dual Somatic Recordings from Gonadotropin-Releasing Hormone (GnRH) Neurons Identified by Green Fluorescent Protein (GFP) in Hypothalamic Slices
02/22/101751An Improved Method of RNA Isolation from Loblolly Pine (P. taeda L.) and Other Conifer Species
02/19/101693Measurement Of Neuromagnetic Brain Function In Pre-school Children With Custom Sized MEG
02/18/101673Fluorescence Activated Cell Sorting of Plant Protoplasts
02/17/101725Single Sensillum Recordings in the Insects Drosophila melanogaster and Anopheles gambiae
02/16/101690Toxin Induction and Protein Extraction from Fusarium spp. Cultures for Proteomic Studies
02/15/101790Studying Synaptic Vesicle Pools using Photoconversion of Styryl Dyes
02/12/101696Live Imaging Of Drosophila melanogaster Embryonic Hemocyte Migrations
02/11/101714Obtaining Hemocytes from the Hawaiian Bobtail Squid Euprymna scolopes and Observing their Adherence to Symbiotic and Non-Symbiotic Bacteria
02/09/101748Preparation of Drosophila Polytene Chromosome Squashes for Antibody Labeling
02/08/101736Imaging Mismatch Repair and Cellular Responses to DNA Damage in Bacillus subtilis
02/05/101487Depletion of Specific Cell Populations by Complement Depletion
02/04/101685Primary Culture and Plasmid Electroporation of the Murine Organ of Corti.
02/03/101726Live Imaging of Cell Motility and Actin Cytoskeleton of Individual Neurons and Neural Crest Cells in Zebrafish Embryos
02/02/101742Using the optokinetic response to study visual function of zebrafish
02/01/101698Split-Ubiquitin Based Membrane Yeast Two-Hybrid (MYTH) System: A Powerful Tool For Identifying Protein-Protein Interactions
01/29/101752Determining the Reactivity and Titre of Serum using a Haemagglutination Assay
01/28/101488Isolation of Mouse Peritoneal Cavity Cells
01/27/101671An Experimental Paradigm for the Prediction of Post-Operative Pain (PPOP)
01/26/101649A Multi-Parametric Islet Perifusion System within a Microfluidic Perifusion Device
01/25/101750Right Hemihepatectomy by Suprahilar Intrahepatic Transection of the Right Hemipedicle using a Vascular Stapler
01/22/101728Surgical Induction of Endolymphatic Hydrops by Obliteration of the Endolymphatic Duct
01/21/101489Scalable Fluidic Injector Arrays for Viral Targeting of Intact 3-D Brain Circuits
01/20/101437Heterotopic and Orthotopic Tracheal Transplantation in Mice used as Models to Study the Development of Obliterative Airway Disease
01/19/101652Murine Colitis Modeling using Dextran Sulfate Sodium (DSS)
01/18/101660Spinal Cord Electrophysiology
01/15/101376Murine Model for Parkinson's Disease: from 6-OH Dopamine Lesion to Behavioral Test
01/14/101704Visualizing RNA Localization in Xenopus Oocytes
01/12/101701A Protocol for the Production of KLRG1 Tetramer
01/11/101634In vivo-like Organotypic Murine Retinal Wholemount Culture
01/08/101707Undecalcified Bone Preparation for Histology, Histomorphometry and Fluorochrome Analysis
01/07/101662Using the Gene Pulser MXcell Electroporation System to Transfect Primary Cells with High Efficiency
01/06/101695Fabrication and Operation of an Oxygen Insert for Adherent Cellular Cultures
01/05/101681Single-cell Suction Recordings from Mouse Cone Photoreceptors
01/04/101676Presynaptically Silent Synapses Studied with Light Microscopy
12/31/091656Automated Microfluidic Blood Lysis Protocol for Enrichment of Circulating Nucleated Cells
12/30/091687A Practical Approach to Genetic Inducible Fate Mapping: A Visual Guide to Mark and Track Cells In Vivo
12/29/091647Live Dissection of Drosophila Embryos: Streamlined Methods for Screening Mutant Collections by Antibody Staining
12/28/091627Engineering Cell-permeable Protein
12/24/091555Freezing and Thawing Human Embryonic Stem Cells
12/23/091669Measuring Plasma Membrane Protein Endocytic Rates by Reversible Biotinylation
12/22/091427Culture and Maintenance of Human Embryonic Stem Cells
12/21/091630Preparation of embryos for Electron Microscopy of the Drosophila embryonic heart tube
12/17/091692PuraMatrix Encapsulation of Cancer Cells
12/16/091653Extraction of the EPP Component from the Surface EMG
12/15/091617A Novel RFP Reporter to Aid in the Visualization of the Eye Imaginal Disc in Drosophila
12/14/091650Lensless On-chip Imaging of Cells Provides a New Tool for High-throughput Cell-Biology and Medical Diagnostics
12/11/091616Osmotic Avoidance in Caenorhabditis elegans: Synaptic Function of Two Genes, Orthologues of Human NRXN1 and NLGN1, as Candidates for Autism
12/10/091614Intranuclear Microinjection of DNA into Dissociated Adult Mammalian Neurons
12/08/091553Generation of Induced Pluripotent Stem Cells by Reprogramming Human Fibroblasts with the Stemgent Human TF Lentivirus Set
12/07/091645Retro-orbital Injection in Adult Zebrafish
12/04/091631Optical Recording of Electrical Activity in Guinea-pig Enteric Networks using Voltage-sensitive Dyes
12/03/091842Using the GELFREE 8100 Fractionation System for Molecular Weight-Based Fractionation with Liquid Phase Recovery
12/02/091622A Rapid High-throughput Method for Mapping Ribonucleoproteins (RNPs) on Human pre-mRNA
12/01/091599Isolation and Purification of Drosophila Peripheral Neurons by Magnetic Bead Sorting
11/30/091620Chick ex ovo Culture and ex ovo CAM Assay: How it Really Works
11/25/091581Assembly, Tuning and Use of an Apertureless Near Field Infrared Microscope for Protein Imaging
11/24/091564Aortic Ring Assay
11/23/091558Methods for the Study of the Zebrafish Maxillary Barbel
11/20/091324Combining Peripheral Nerve Grafting and Matrix Modulation to Repair the Injured Rat Spinal Cord
11/19/091659Measuring the Induced Membrane Voltage with Di-8-ANEPPS
11/18/091550The use of SC1 (Pluripotin) to Support mESC Self-renewal in the Absence of LIF
11/17/091543Dissection of Hippocampal Dentate Gyrus from Adult Mouse
11/16/091596Monitoring Heart Function in Larval Drosophila melanogaster for Physiological Studies
11/13/091447Generation of Induced Pluripotent Stem Cells by Reprogramming Mouse Embryonic Fibroblasts with a Four Transcription Factor, Doxycycline Inducible Lentiviral Transduction System
11/12/091554The use of Biofeedback in Clinical Virtual Reality: The INTREPID Project
11/10/091569Extraction of High Molecular Weight Genomic DNA from Soils and Sediments
11/09/091595Historical View and Physiology Demonstration at the NMJ of the Crayfish Opener Muscle
11/06/091603Digital Microfluidics for Automated Proteomic Processing
11/05/091530Assembly, Loading, and Alignment of an Analytical Ultracentrifuge Sample Cell
11/04/091610Microvolume Protein Concentration Determination using the NanoDrop 2000c Spectrophotometer
11/03/091420Obtaining Highly Purified Toxoplasma gondii Oocysts by a Discontinuous Cesium Chloride Gradient
11/02/091542Automated System for Single Molecule Fluorescence Measurements of Surface-immobilized Biomolecules
10/30/091523Preparation of Pooled Human Platelet Lysate (pHPL) as an Efficient Supplement for Animal Serum-Free Human Stem Cell Cultures
10/29/091485Denaturing Urea Polyacrylamide Gel Electrophoresis (Urea PAGE)
10/28/091524Isolation and Large Scale Expansion of Adult Human Endothelial Colony Forming Progenitor Cells
10/27/091644Platelet Adhesion and Aggregation Under Flow using Microfluidic Flow Cells
10/26/091590Cellular Encapsulation in 3D Hydrogels for Tissue Engineering
10/23/091588ELIME (Enzyme Linked Immuno Magnetic Electrochemical) Method for Mycotoxin Detection
10/22/091635Isolation and Derivation of Mouse Embryonic Germinal Cells
10/21/091589Electrospinning Fibrous Polymer Scaffolds for Tissue Engineering and Cell Culture
10/20/091592Noninvasive In Vivo Small Animal MRI and MRS: Basic Experimental Procedures
10/19/091520DNA Transfection of Mammalian Skeletal Muscles using In Vivo Electroporation
10/16/091373Generation of Human CD40-activated B cells
10/15/091594Measures of Heart and Ventilatory Rates in Freely Moving Crayfish
10/14/091438LAD-Ligation: A Murine Model of Myocardial Infarction
10/13/091423Fluorescent Labeling of Drosophila Heart Structures
10/12/091474Quantitative Phosphoproteomics in Fatty Acid Stimulated Saccharomyces cerevisiae
10/09/091529Visualizing Single-molecule DNA Replication with Fluorescence Microscopy
10/08/091525Isolation and Animal Serum Free Expansion of Human Umbilical Cord Derived Mesenchymal Stromal Cells (MSCs) and Endothelial Colony Forming Progenitor Cells (ECFCs)
10/07/091549Antibody Profiling by Luciferase Immunoprecipitation Systems (LIPS)
10/06/091265Mouse Mammary Epithelial Cells form Mammospheres During Lactogenic Differentiation
10/05/091548Anterior Cervical Discectomy and Fusion in the Ovine Model
10/02/091499Lentivirus Production
10/01/091398A Lectin HPLC Method to Enrich Selectively-glycosylated Peptides from Complex Biological Samples
09/30/091304Preparation of Rat Brain Aggregate Cultures for Neuron and Glia Development Studies
09/29/091404Preparation and Using Phantom Lesions to Practice Fine Needle Aspiration Biopsies
09/28/091425 Visualizing the Beating Heart in Drosophila
09/25/091294Microsurgical Clip Obliteration of Middle Cerebral Aneurysm Using Intraoperative Flow Assessment
09/24/091333In utero and ex vivo Electroporation for Gene Expression in Mouse Retinal Ganglion Cells
09/23/091387Large Insert Environmental Genomic Library Production
09/22/091519Preparation and Culture of Rat Lens Epithelial Explants for Studying Terminal Differentiation
09/21/091388Tracking Dynamics of Muscle Engraftment in Small Animals by In Vivo Fluorescent Imaging
09/18/091352DNA Extraction from 0.22 μM Sterivex Filters and Cesium Chloride Density Gradient Centrifugation
09/17/091433Isolation and Genetic Manipulation of Adult Cardiac Myocytes for Confocal Imaging
09/16/091435Semi-automated Optical Heartbeat Analysis of Small Hearts
09/15/091382Microinjection Techniques for Studying Mitosis in the Drosophila melanogaster Syncytial Embryo
09/14/091346Imaging In-Stent Restenosis: An Inexpensive, Reliable, and Rapid Preclinical Model
09/11/091422Tissue Targeted Embryonic Chimeras: Zebrafish Gastrula Cell Transplantation
09/10/091399A Multi-compartment CNS Neuron-glia Co-culture Microfluidic Platform
09/09/091442Assay for Pathogen-Associated Molecular Pattern (PAMP)-Triggered Immunity (PTI) in Plants
09/08/091508Visualizing Single Molecular Complexes In Vivo Using Advanced Fluorescence Microscopy
09/07/091484Method for Measurement of Viral Fusion Kinetics at the Single Particle Level
09/04/091512Chromosomal Spread Preparation of Human Embryonic Stem Cells for Karyotyping
09/03/091573Primer Extension Capture: Targeted Sequence Retrieval from Heavily Degraded DNA Sources
09/02/091329High Speed Droplet-based Delivery System for Passive Pumping in Microfluidic Devices
09/01/091405Fluorescence in situ hybridization (FISH) Protocol in Human Sperm
08/31/091562Dopamine Release at Individual Presynaptic Terminals Visualized with FFNs
08/28/091385Bioluminescence Imaging of Heme Oxygenase-1 Upregulation in the Gua Sha Procedure
08/27/091509In vivo Imaging of Deep Cortical Layers using a Microprism
08/26/091432Combining QD-FRET and Microfluidics to Monitor DNA Nanocomplex Self-Assembly in Real-Time
08/25/091390Antifouling Self-assembled Monolayers on Microelectrodes for Patterning Biomolecules
08/24/091417Purification of Mitochondria from Yeast Cells
08/21/091513A Quantitative Assessment of The Yeast Lipidome using Electrospray Ionization Mass Spectrometry
08/20/091510Mesoscopic Fluorescence Tomography for In-vivo Imaging of Developing Drosophila
08/19/091293Detection of Protein Ubiquitination
08/18/091353In vivo Ca2+- Imaging of Mushroom Body Neurons During Olfactory Learning in the Honey Bee
08/17/091149Isolation of Protoplasts from Tissues of 14-day-old Seedlings of Arabidopsis thaliana
08/14/091350Staining of Proteins in Gels with Coomassie G-250 without Organic Solvent and Acetic Acid
08/13/091561A Technique to Simultaneously Visualize Virus-Specific CD8+ T Cells and Virus-Infected Cells In situ
08/12/091274Mating and Tetrad Separation of Chlamydomonas reinhardtii for Genetic Analysis
08/11/091138Optical Mapping of Langendorff-perfused Rat Hearts
08/10/091471Global Gene Expression Analysis Using a Zebrafish Oligonucleotide Microarray Platform
08/07/091470RNA Isolation from Embryonic Zebrafish and cDNA Synthesis for Gene Expression Analysis
08/06/091444Obtaining High Quality RNA from Single Cell Populations in Human Postmortem Brain Tissue
08/05/091325Live Cell Imaging of F-actin Dynamics via Fluorescent Speckle Microscopy (FSM)
08/04/091514Use of the Protease Fluorescent Detection Kit to Determine Protease Activity
08/03/091431Fast and Sensitive Colloidal Coomassie G-250 Staining for Proteins in Polyacrylamide Gels
07/31/091443Psychophysiological Stress Assessment Using Biofeedback
07/30/091309Making MR Imaging Child's Play - Pediatric Neuroimaging Protocol, Guidelines and Procedure
07/29/091319Using an EEG-Based Brain-Computer Interface for Virtual Cursor Movement with BCI2000
07/28/091268Exploring Cognitive Functions in Babies, Children & Adults with Near Infrared Spectroscopy
07/27/091286Batch Immunostaining for Large-Scale Protein Detection in the Whole Monkey Brain
07/24/091260Brain Banking: Making the Most of your Research Specimens
07/23/091280Creation of Murine Experimental Abdominal Aortic Aneurysms with Elastase
07/22/091297Recordings of Neural Circuit Activation in Freely Behaving Animals
07/21/091316Cell Block Preparation from Cytology Specimen with Predominance of Individually Scattered Cells
07/20/091475An In vitro FluoroBlok Tumor Invasion Assay
07/17/091394Generating Chimeric Zebrafish Embryos by Transplantation
07/16/091259Dissecting the Non-human Primate Brain in Stereotaxic Space
07/15/091237Bioelectric Analyses of an Osseointegrated Intelligent Implant Design System for Amputees
07/13/091366An In Vitro Skin Irritation Test (SIT) using the EpiDerm Reconstructed Human Epidermal (RHE) Model
07/10/091150Murine Renal Transplantation Procedure
07/09/091155Live Imaging of Glial Cell Migration in the Drosophila Eye Imaginal Disc
07/07/091267Generation of Single-Cell Suspensions from Mouse Neural Tissue
07/06/091395A High-Throughput Method For Zebrafish Sperm Cryopreservation and In Vitro Fertilization
07/03/091384Using the Horseshoe Crab, Limulus Polyphemus, in Vision Research
07/02/091266Standardized Preparation of Single-Cell Suspensions from Mouse Lung Tissue using the gentleMACS Dissociator
06/30/091396Making Gynogenetic Diploid Zebrafish by Early Pressure
06/29/091247Focal Ca2+ Transient Detection in Smooth Muscle
06/25/091209Measuring Replicative Life Span in the Budding Yeast
06/24/091139Implantation of Engineered Tissue in the Rat Heart
06/23/091232Demonstration of Cutaneous Allodynia in Association with Chronic Pelvic Pain
06/19/091163Small Volume (1-3L) Filtration of Coastal Seawater Samples
06/18/091161Large Volume (20L+) Filtration of Coastal Seawater Samples
06/17/091159Seawater Sampling and Collection
06/16/091308Primary Culture of Adult Rat Heart Myocytes
06/15/091127Monitoring Plant Hormones During Stress Responses
06/12/091204The Ladder Rung Walking Task: A Scoring System and its Practical Application.
06/11/091301Catheterization of Intestinal Loops in Ruminants
06/10/091292Virus-induced Gene Silencing (VIGS) in Nicotiana benthamiana and Tomato
06/09/091305Imaging Exocytosis in Retinal Bipolar Cells with TIRF Microscopy
06/08/091355Preparation of Aplysia Sensory-motor Neuronal Cell Cultures
06/05/091278Dendra2 Photoswitching through the Mammary Imaging Window
06/04/091418The Microfluidic Probe: Operation and Use for Localized Surface Processing
06/03/091374A Tactile Automated Passive-Finger Stimulator (TAPS)
06/02/091389Born Normalization for Fluorescence Optical Projection Tomography for Whole Heart Imaging
06/01/091258Lens Transplantation in Zebrafish and its Application in the Analysis of Eye Mutants
05/29/091144Live Imaging of Dense-core Vesicles in Primary Cultured Hippocampal Neurons
05/28/091171Homarus Americanus Stomatogastric Nervous System Dissection
05/27/091261The Gateway to the Brain: Dissecting the Primate Eye
05/26/091343Human Pancreatic Islet Isolation: Part II: Purification and Culture of Human Islets
05/26/091125Human Pancreatic Islet Isolation: Part I: Digestion and Collection of Pancreatic Tissue
05/22/091320Gross Dissection of the Stomach of the Lobster, Homarus Americanus
05/21/091348Electrophysiological Recording in the Drosophila Embryo
05/20/091347Harvesting and Preparing Drosophila Embryos for Electrophysiological Recording and Other Procedures
05/19/091146Dissection of Saccharomyces Cerevisiae Asci
05/18/091178Proteomics to Identify Proteins Interacting with P2X2 Ligand-Gated Cation Channels
05/15/091291Measuring Blood Pressure in Mice using Volume Pressure Recording, a Tail-cuff Method
05/14/091262Knowing What Counts: Unbiased Stereology in the Non-human Primate Brain
05/13/091154Visualizing the Live Drosophila Glial-neuromuscular Junction with Fluorescent Dyes
05/12/091152Measuring Caenorhabditis elegans Life Span on Solid Media
05/11/091135Homemade Site Directed Mutagenesis of Whole Plasmids
05/08/091211Gross and Fine Dissection of Inner Ear Sensory Epithelia in Adult Zebrafish (Danio rerio)
05/07/091113Microinjection of mRNA and Morpholino Antisense Oligonucleotides in Zebrafish Embryos.
05/06/091156Quantifying Yeast Chronological Life Span by Outgrowth of Aged Cells
05/05/091173A Neuronal and Astrocyte Co-Culture Assay for High Content Analysis of Neurotoxicity
05/04/091040Fabrication of Amperometric Electrodes
05/01/091137Fabrication of Myogenic Engineered Tissue Constructs
04/29/091210In vivo Bioluminescent Imaging of Mammary Tumors Using IVIS Spectrum
04/26/091142Measuring Near Plasma Membrane and Global Intracellular Calcium Dynamics in Astrocytes
04/24/091193A Video Demonstration of Preserved Piloting by Scent Tracking but Impaired Dead Reckoning After Fimbria-Fornix Lesions in the Rat
04/22/091098Micro-drive Array for Chronic in vivo Recording: Tetrode Assembly
04/20/091094Micro-drive Array for Chronic in vivo Recording: Drive Fabrication
04/17/091205Non-radioactive in situ Hybridization Protocol Applicable for Norway Spruce and a Range of Plant Species
04/15/091121Neurocircuit Assays for Seizures in Epilepsy Mutants of Drosophila
04/13/091170Vaccinia Virus Infection & Temporal Analysis of Virus Gene Expression: Part 3
04/10/091169Vaccinia Virus Infection & Temporal Analysis of Virus Gene Expression: Part 2
04/08/091168Vaccinia Virus Infection & Temporal Analysis of Virus Gene Expression: Part 1
04/06/091218Zebrafish Brain Ventricle Injection
04/03/091191Cellular Toxicity of Nanogenomedicine in MCF-7 Cell Line: MTT assay
04/01/091217Live Imaging of the Zebrafish Embryonic Brain by Confocal Microscopy
03/28/091108Drosophila Larval NMJ Immunohistochemistry
03/27/091120Collection and Cryopreservation of Hamster Oocytes and Mouse Embryos
03/25/091229Zebrafish Whole Mount High-Resolution Double Fluorescent In Situ Hybridization
03/23/091207Cancer Borealis Stomatogastric Nervous System Dissection
03/20/091182Processing the Loblolly Pine PtGen2 cDNA Microarray
03/18/091104Homing of Hematopoietic Cells to the Bone Marrow
03/16/091133Channelrhodopsin2 Mediated Stimulation of Synaptic Potentials at Drosophila Neuromuscular Junctions
03/13/091206The Preparation of Drosophila Embryos for Live-Imaging Using the Hanging Drop Protocol
03/11/091263Measuring Plant Cell Wall Extension (Creep) Induced by Acidic pH and by Alpha-Expansin
03/09/091115Microinjection of Zebrafish Embryos to Analyze Gene Function
03/06/091167Visualizing Antigen Specific CD4+ T Cells using MHC Class II Tetramers
03/04/091225Identification of protein complexes with quantitative proteomics in S. cerevisiae
03/02/091224A high-throughput method to globally study the organelle morphology in S. cerevisiae
02/27/091118Laparoscopic Left Liver Sectoriectomy of Caroli's Disease Limited to Segment II and III
02/25/091165Dissecting and Recording from The C. Elegans Neuromuscular Junction
02/23/091106Microinjection of Xenopus Laevis Oocytes
02/20/091096High-Resolution Video Tracking of Locomotion in Adult Drosophila Melanogaster
02/18/091086Analyzing Gene Expression from Marine Microbial Communities using Environmental Transcriptomics
02/16/091129Two-photon axotomy and time-lapse confocal imaging in live zebrafish embryos
02/13/091027Assay for Neural Induction in the Chick Embryo
02/11/091009Analysis of Physiologic E-Selectin-Mediated Leukocyte Rolling on Microvascular Endothelium
02/09/091105Purification and Visualization of Influenza A Viral Ribonucleoprotein Complexes
02/06/091109Electrophysiological Methods for Recording Synaptic Potentials from the NMJ of Drosophila Larvae
02/04/091107Drosophila Larval NMJ Dissection
02/02/09956Method for Whole Mount Antibody Staining in Chick
01/30/09988Protocol for Culturing Sympathetic Neurons from Rat Superior Cervical Ganglia (SCG)
01/28/09949Olfactory Behavioral Testing in the Adult Mouse
01/26/091110Visually Mediated Odor Tracking During Flight in Drosophila
01/23/091034Embryonic Stem Cell-Derived Endothelial Cells for Treatment of Hindlimb Ischemia
01/21/091035Murine Model of Hindlimb Ischemia
01/19/091067Calcium Imaging of Cortical Neurons using Fura-2 AM
01/16/09990Isolation and Culture of Post-Natal Mouse Cerebellar Granule Neuron Progenitor Cells and Neurons
01/14/091092Determining heat and mechanical pain threshold in inflamed skin of human subjects
01/12/091071Photo-Induced Cross-Linking of Unmodified Proteins (PICUP) Applied to Amyloidogenic Peptides
01/06/091026The Preparation of Primary Hematopoietic Cell Cultures From Murine Bone Marrow for Electroporation
01/02/09935Methylated DNA Immunoprecipitation
12/22/081088Elevated Plus Maze for Mice
12/18/081037Detection and Isolation of Viable Mouse IL-17-Secreting T Cells
12/15/081038Electrochemotherapy of Tumours
12/11/081029Preparation of Single-Cell Suspensions from Mouse Spleen with the gentleMACS Dissociator
12/10/081030Use of Rotorod as a Method for the Qualitative Analysis of Walking in Rat
12/05/081065Microcontact Printing of Proteins for Cell Biology
12/01/081074Human In-Vivo Bioassay for the Tissue-Specific Measurement of Nociceptive and Inflammatory Mediators
11/26/08945Selective Labelling of Cell-surface Proteins using CyDye DIGE Fluor Minimal Dyes
11/21/081063A Magnetic Tether System to Investigate Visual and Olfactory Mediated Flight Control in Drosophila
11/19/08955The Hypoxic Ischemic Encephalopathy Model of Perinatal Ischemia
11/13/08992Functional Imaging with Reinforcement, Eyetracking, and Physiological Monitoring
11/11/08942Optimization of the Ugi Reaction Using Parallel Synthesis and Automated Liquid Handling
11/10/08960A Technique for Serial Collection of Cerebrospinal Fluid from the Cisterna Magna in Mouse
11/05/08820Primary Dissociated Midbrain Dopamine Cell Cultures from Rodent Neonates
11/03/081032Single Molecule Methods for Monitoring Changes in Bilayer Elastic Properties
10/31/081033Preparation of Artificial Bilayers for Electrophysiology Experiments
10/29/08962Collecting and Measuring Nociceptive and Inflammatory Mediators in Surgical Wounds
10/27/08904Double Whole Mount in situ Hybridization of Early Chick Embryos
10/22/08964Pressure-polishing Pipettes for Improved Patch-clamp Recording
10/20/08903Method for Culture of Early Chick Embryos ex vivo (New Culture)
10/17/08951Preparation and Maintenance of Dorsal Root Ganglia Neurons in Compartmented Cultures
10/16/08936Patch Clamp Recording of Ion Channels Expressed in Xenopus Oocytes
10/13/08958Blood Collection from the American Horseshoe Crab, Limulus Polyphemus
10/08/08939Making Patch-pipettes and Sharp Electrodes with a Programmable Puller
10/06/08880Survivable Stereotaxic Surgery in Rodents
10/03/08923A Behavioral Assay to Measure Responsiveness of Zebrafish to Changes in Light Intensities
10/01/08914Neuronal Nuclei Isolation from Human Postmortem Brain Tissue
09/29/08895Primary Culture of Hippocampal Neurons from P0 Newborn Rats
09/24/08897Morris Water Maze Experiment
09/17/08899Sigma's Non-specific Protease Activity Assay - Casein as a Substrate
09/15/08947Supported Planar Bilayers for the Formation of Study of Immunological Synapses and Kinapse
09/11/08911Using Laser Tweezers For Manipulating Isolated Neurons In Vitro
09/07/08920Studying Membrane Biogenesis with a Luciferase-Based Reporter Gene Assay
09/03/08888Protocols for Oral Infection of Lepidopteran Larvae with Baculovirus
09/02/08908In Vitro Nuclear Assembly Using Fractionated Xenopus Egg Extracts
08/28/08906Preparation of 2-dGuo-Treated Thymus Organ Cultures
08/28/08905Reaggregate Thymus Cultures
08/27/08891Preparation and Fractionation of Xenopus laevis Egg Extracts
08/23/08889Protocols for Microapplicator-assisted Infection of Lepidopteran Larvae with Baculovirus
08/20/08890Obtaining Eggs from Xenopus laevis Females
08/18/08837Paradigms for Pharmacological Characterization of C. elegans Synaptic Transmission Mutants
08/15/08833Generation of Stable Transgenic C. elegans Using Microinjection
08/14/08907Imaging Effector Memory T cells in the Ear After Induction of Adoptive DTH
08/11/08842Proper Care and Cleaning of the Microscope
08/11/08845The MODS method for diagnosis of tuberculosis and multidrug resistant tuberculosis
08/08/08816The Structure of Skilled Forelimb Reaching in the Rat: A Movement Rating Scale
08/06/08844Phase Contrast and Differential Interference Contrast (DIC) Microscopy
08/05/08870Technical Demonstration of Whole Genome Array Comparative Genomic Hybridization
08/03/08827In vitro Labeling of Human Embryonic Stem Cells for Magnetic Resonance Imaging
07/30/08843Major Components of the Light Microscope
07/28/08813AC Electrokinetic Phenomena Generated by Microelectrode Structures
07/25/08769Culture of myeloid dendritic cells from bone marrow precursors
07/23/08825In vitro Differentiation of Mouse Embryonic Stem (mES) Cells Using the Hanging Drop Method
07/23/08745Neutrophil Isolation Protocol
07/22/08780Chromatin Immunoprecipitation from Human Embryonic Stem Cells
07/18/08835Application of a C. elegans Dopamine Neuron Degeneration Assay for the Validation of Potential Parkinson's Disease Genes
07/16/08838Screening for Amyloid Aggregation by Semi-Denaturing Detergent-Agarose Gel Electrophoresis
07/16/08786Interview: Protein Folding and Studies of Neurodegenerative Diseases
07/11/08705Single Cell Electroporation in vivo within the Intact Developing Brain
07/09/08773Generation of Bone Marrow Derived Murine Dendritic Cells for Use in 2-photon Imaging
07/08/08760Staining Proteins in Gels
07/02/08771Whole-cell Recordings of Light Evoked Excitatory Synaptic Currents in the Retinal Slice
07/02/08757Freezing, Thawing, and Packaging Cells for Transport
06/27/08789Isolation of Early Hematopoietic Stem Cells from Murine Yolk Sac and AGM
06/24/08742Isolation and Analysis of Hematopoietic Stem Cells from the Placenta
06/23/08752Counting and Determining the Viability of Cultured Cells
06/20/08759Immunoblot Analysis
06/17/08724Probing for Mitochondrial Complex Activity in Human Embryonic Stem Cells
06/17/08819Retrograde Labeling of Retinal Ganglion Cells by Application of Fluoro-Gold on the Surface of Superior Colliculus
06/16/08731Operant Learning of Drosophila at the Torque Meter
06/16/08793Interview: HIV-1 Proviral DNA Excision Using an Evolved Recombinase
06/12/08758Electrophoretic Separation of Proteins
06/12/08755Trypsinizing and Subculturing Mammalian Cells
06/10/08832From MEFs to Matrigel 3: Passaging hESCs from Matrigel onto Matrigel
06/09/08831From MEFs to Matrigel 2: Splitting hESCs from MEFs onto Matrigel
06/04/08722From MEFs to Matrigel I: Passaging hESCs in the Presence of MEFs
05/31/08709Use of Arabidopsis eceriferum Mutants to Explore Plant Cuticle Biosynthesis
05/29/08791Molecular Evolution of the Tre Recombinase
05/29/08718Principles of Site-Specific Recombinase (SSR) Technology
05/27/08737Non-invasive 3D-Visualization with Sub-micron Resolution Using Synchrotron-X-ray-tomography
05/23/08761Electroporation of Mycobacteria
05/21/08564Chip-based Three-dimensional Cell Culture in Perfused Micro-bioreactors
05/21/08792Interview: Bioreactors and Surfaced-Modified 3D-Scaffolds for Stem Cell Research
05/14/08701Testing Nicotine Tolerance in Aphids Using an Artificial Diet Experiment
05/14/08700Testing the Physiological Barriers to Viral Transmission in Aphids Using Microinjection
05/14/08683Choice and No-Choice Assays for Testing the Resistance of A. thaliana to Chewing Insects
05/12/08699Microfabrication of Chip-sized Scaffolds for Three-dimensional Cell cultivation
05/09/08772Mouse Epidermal Neural Crest Stem Cell (EPI-NCSC) Cultures
05/02/08740In vitro and in vivo Bioluminescence Reporter Gene Imaging of Human Embryonic Stem Cells
04/28/08751Titration of Human Coronaviruses Using an Immunoperoxidase Assay
04/17/08697Localized RNAi and Ectopic Gene Expression in the Medicinal Leech
04/07/08734Generating iPS Cells from MEFS through Forced Expression of Sox-2, Oct-4, c-Myc, and Klf4
04/02/08686 Labeling Stem Cells with Fluorescent Dyes for non-invasive Detection with Optical Imaging
03/31/08685Labeling hESCs and hMSCs with Iron Oxide Nanoparticles for Non-Invasive in vivo Tracking with MR Imaging
03/28/08690Actin Co-Sedimentation Assay; for the Analysis of Protein Binding to F-Actin
03/21/08717A Chromatin Assay for Human Brain Tissue
03/17/08708Targeted Expression of GFP in the Hair Follicle Using Ex Vivo Viral Transduction
03/17/08716Construction and Implantation of a Microinfusion System for Sustained Delivery of Neuroactive Agents.
03/13/08681In Vivo 2-Photon Calcium Imaging in Layer 2/3 of Mice
03/05/08692Shrinky-Dink Hanging Drops: A Simple Way to Form and Culture Embryoid Bodies
02/25/08678A Method for 2-Photon Imaging of Blood Flow in the Neocortex through a Cranial Window
02/20/08662Neuronal Cell Cultures from Aplysia for High-Resolution Imaging of Growth Cones
02/15/08680A Craniotomy Surgery Procedure for Chronic Brain Imaging
02/13/08675Preparation of Gene Gun Bullets and Biolistic Transfection of Neurons in Slice Culture
02/11/08674Assessing Neural Stem Cell Motility Using an Agarose Gel-based Microfluidic Device
02/03/08664Fabrication of the Thermoplastic Microfluidic Channels
02/03/08665Microfluidic Applications for Disposable Diagnostics
01/25/08652In situ Imaging of the Mouse Thymus Using 2-Photon Microscopy
01/22/08636Rapid Genotyping of Mouse Tissue Using Sigma's Extract-N-Amp Tissue PCR Kit
01/16/08634Murine Skin Transplantation
01/08/08552Immunohistochemistry on Paraffin Sections of Mouse Epidermis Using Fluorescent Antibodies
01/08/08630Pyrosequencing: A Simple Method for Accurate Genotyping
01/03/08638High-resolution Measurement of Odor-Driven Behavior in Drosophila Larvae
12/31/07635Interview: Glycolipid Antigen Presentation by CD1d and the Therapeutic Potential of NKT cell Activation
12/19/07562Preparation of Dissociated Mouse Cortical Neuron Cultures
12/13/07574Derivation of Human Embryonic Stem Cells by Immunosurgery
12/10/07556A Method For Production of Recombinant mCD1d Protein in Insect Cells.
12/04/07527Isolation of Mononuclear Cells from the Central Nervous System of Rats with EAE
12/04/07550Production of Replication-Defective Retrovirus by Transient Transfection of 293T cells
12/04/07484Orthotopic Mouse Model of Colorectal Cancer
12/04/07549Laser-Induced Chronic Ocular Hypertension Model on SD Rats
11/01/07412Issue 9: Transplantation, Electroporation, and more...
11/01/07411BioMEMS: Forging New Collaborations Between Biologists and Engineers
11/01/07410Non-plasma Bonding of PDMS for Inexpensive Fabrication of Microfluidic Devices
11/01/07409Isolation of CD4+ T cells from Mouse Lymph Nodes Using Miltenyi MACS Purification
11/01/07408In Ovo Electroporations of HH Stage 10 Chicken Embryos
11/01/07407Understanding Cerebellar Pattern Formation
10/31/07405RNA Extraction from Neuroprecursor Cells Using the Bio-Rad Total RNA Kit
10/31/07404Transplantation of Pancreatic Islets Into the Kidney Capsule of Diabetic Mice
10/31/07402Preparing T Cell Growth Factor from Rat Splenocytes
10/01/07323Development of New Therapeutic Applications Using Microfluidics
10/01/07320Cell Capture Using a Microfluidic Device
10/01/07319PDMS Device Fabrication and Surface Modification
10/01/07318Patterning of Embryonic Stem Cells Using the Bio Flip Chip
10/01/07317Micro-scale Engineering for Cell Biology
10/01/07316Title Cell Encapsulation by Droplets
10/01/07315CD4+ T-Lymphocyte Capture Using a Disposable Microfluidic Chip for HIV
10/01/07314Using Micro-Electro-Mechanical Systems (MEMS) to Develop Diagnostic Tools
10/01/07313Intravitreous Injection for Establishing Ocular Diseases Model
10/01/07311Microfabricated Post-Array-Detectors (mPADs): an Approach to Isolate Mechanical Forces
10/01/07324JoVE Issue 8: Microfluidics, cell patterning and more.
10/01/07325Induction and Monitoring of Adoptive Delayed-Type Hypersensitivity in Rats
10/01/07326Enrichment of NK Cells from Human Blood with the RosetteSep Kit from StemCell Technologies
10/01/07296Microfluidic Chips Controlled with Elastomeric Microvalve Arrays
10/01/07300BioMEMS and Cellular Biology: Perspectives and Applications
10/01/07301Applying Microfluidics to Electrophysiology
10/01/07302Brain Slice Stimulation Using a Microfluidic Network and Standard Perfusion Chamber
10/01/07305Preparing E18 Cortical Rat Neurons for Compartmentalization in a Microfluidic Device
10/01/07306Windowing Chicken Eggs for Developmental Studies
10/01/07307Placing Growth Factor-Coated Beads on Early Stage Chicken Embryos
10/01/07308Immunohistochemistry: Paraffin Sections Using the Vectastain ABC Kit from Vector Labs
10/01/07309Laser Capture Microdissection of Mammalian Tissue
08/30/07268Silicon Microchips for Manipulating Cell-cell Interaction
08/30/07269Micro-dissection of Rat Brain for RNA or Protein Extraction from Specific Brain Region
08/30/07270A Microfluidic Device with Groove Patterns for Studying Cellular Behavior
08/30/07271A Gradient-generating Microfluidic Device for Cell Biology
08/30/07272Experimental Approaches to Tissue Engineering
08/30/07274JoVE 7th Issue: Bioengineering and Immunology
08/24/07267Immunocytochemistry: Human Neural Stem Cells
08/23/07265Dissection and 2-Photon Imaging of Peripheral Lymph Nodes in Mice
08/23/07266Drawing Blood from Rats through the Saphenous Vein and by Cardiac Puncture
08/22/07255Murine Pancreatic Islet Isolation
08/22/07261Fabrication of a Microfluidic Device for the Compartmentalization of Neuron Soma and Axons
08/22/07262Counting Human Neural Stem Cells
08/22/07263Passaging Human Neural Stem Cells
08/22/07264Western Blotting Using the Invitrogen NuPage Novex Bis Tris MiniGels
08/20/07256Investigating the Immunological Mechanisms Underlying Organ Transplant Rejection
08/20/07257Regulatory T cells: Therapeutic Potential for Treating Transplant Rejection and Type I Diabetes
08/20/07258Small Bowel Transplantation In Mice
08/20/07259Patterning Cells on Optically Transparent Indium Tin Oxide Electrodes
08/01/07253Transformation of Plasmid DNA into E. coli Using the Heat Shock Method
07/31/07251Perspectives on Neuroscience
07/30/07254JoVE 6th Issue
07/30/07249Application of Light-cured Dental Adhesive Resin for Mounting Electrodes or Microdialysis Probes in Chronic Experiments
07/30/07250Loading Drosophila Nerve Terminals with Calcium Indicators
07/29/07242Mechanical Stimulation of Stem Cells Using Cyclic Uniaxial Strain
07/29/07243Computer-Generated Animal Model Stimuli
07/29/07244Methods for Patch Clamp Capacitance Recordings from the Calyx
07/29/07245ALS - Motor Neuron Disease: Mechanism and Development of New Therapies
07/29/07246Isolation of Genomic DNA from Mouse Tails
07/29/07247Purifying Plasmid DNA from Bacterial Colonies Using the Qiagen Miniprep Kit
07/29/07248Whole Cell Recordings from Brain of Adult Drosophila
07/19/07236In Utero Intraventricular Injection and Electroporation of E16 Rat Embryos
07/19/07237Induction and Monitoring of Active Delayed Type Hypersensitivity (DTH) in Rats
07/19/07238Heterotopic Heart Transplantation in Mice
07/19/07239In Utero Intraventricular Injection and Electroporation of E15 Mouse Embryos
07/19/07240Transfecting Human Neural Stem Cells with the Amaxa Nucleofector
07/19/07241Cortical Neurogenesis: Transitioning from Advances in the Laboratory to Cell-Based Therapies
07/11/07235Organotypic Slice Culture of E18 Rat Brains
07/05/07234JoVE 5th Issue: Mosquito Biology
07/04/07220Protocol for Dengue Infections in Mosquitoes (A. aegypti) and Infection Phenotype Determination
07/04/07221Protocol for Mosquito Rearing (A. gambiae)
07/04/07222Protocol for Plasmodium falciparum Infections in Mosquitoes and Infection Phenotype Determination
07/04/07223Maintaining Wolbachia in Cell-free Medium
07/04/07224Induction and Clinical Scoring of Chronic-Relapsing Experimental Autoimmune Encephalomyelitis
07/04/07225Population Replacement Strategies for Controlling Vector Populations and the Use of Wolbachia pipientis for Genetic Drive
07/04/07226Preparation of Neuronal Cultures from Midgastrula Stage Drosophila Embryos
07/04/07227Predicting the Effectiveness of Population Replacement Strategy Using Mathematical Modeling
07/04/07228Dissection of Midgut and Salivary Glands from Ae. aegypti Mosquitoes
07/04/07230Protocol for RNAi Assays in Adult Mosquitoes (A. gambiae)
07/04/07231Preventing the Spread of Malaria and Dengue Fever Using Genetically Modified Mosquitoes
07/04/07233Building a Better Mosquito: Identifying the Genes Enabling Malaria and Dengue Fever Resistance in A. gambiae and A. aegypti Mosquitoes
07/04/07215Injection of dsRNA into Female A. aegypti Mosquitos
07/04/07216Injection of An. stephensi Embryos to Generate Malaria-resistant Mosquitoes
07/04/07219Microinjection of A. aegypti Embryos to Obtain Transgenic Mosquitoes
06/02/07212JoVE 4th Issue
05/28/07195Extracting DNA from the Gut Microbes of the Termite (Zootermopsis Angusticollis) and Visualizing Gut Microbes
05/28/07196Investigating the Microbial Community in the Termite Hindgut - Interview
05/28/07197Layers of Symbiosis - Visualizing the Termite Hindgut Microbial Community
05/28/07198Flash Freezing and Cryosectioning E12.5 Mouse Brain
05/28/07200Primary Neuronal Cultures from the Brains of Late Stage Drosophila Pupae
05/28/07201Large-Scale Screens of Metagenomic Libraries
05/28/07202Microbial Communities in Nature and Laboratory - Interview
05/28/07203Chemotactic Response of Marine Micro-Organisms to Micro-Scale Nutrient Layers
05/28/07204Studies of Bacterial Chemotaxis Using Microfluidics - Interview
05/28/07205Biology of Microbial Communities - Interview
05/28/07206Bacterial Gene Expression Analysis Using Microarrays
05/28/07207Vibrio cholerae: Model Organism to Study Bacterial Pathogenesis - Interview
05/28/07208In situ Protocol for Butterfly Pupal Wings Using Riboprobes
05/28/07209Ex vivo Mechanical Loading of Tendon
05/28/07210In vivo Micro-circulation Measurement in Skeletal Muscle by Intra-vital Microscopy
05/01/07178Jove 3rd Issue
04/29/07186Optimized Fibrin Gel Bead Assay for the Study of Angiogenesis
04/29/07187Dissection of Organizer and Animal Pole Explants from Xenopus laevis Embryos and Assembly of a Cell Adhesion Assay
04/29/07188Plastic Embedding and Sectioning of Xenopus laevis Embryos
04/29/07189Ole Isacson: Development of New Therapies for Parkinson's Disease
04/29/07190Organotypic Culture of Adult Rabbit Retina
04/29/07192MALDI Sample Preparation: the Ultra Thin Layer Method
04/29/07193Proboscis Extension Response (PER) Assay in Drosophila
04/28/07194Thin Sectioning of Slice Preparations for Immunohistochemistry
04/28/07183Isolation of Human Umbilical Vein Endothelial Cells (HUVEC)
04/28/07184Harvesting Sperm and Artificial Insemination of Mice
04/28/07185Retrieval of Mouse Oocytes
04/28/07175Christopher Hughes: An in vitro model for the Study of Angiogenesis (Interview)
02/25/07152Culture of Mouse Neural Stem Cell Precursors
02/25/07155Studying Aggression in Drosophila (fruit flies)
02/25/07156Interview with Edward Kravitz
02/25/07157Isolation and Transplantation of Hematopoietic Stem Cells (HSCs)
02/25/07158Wolbachia Bacterial Infection in Drosophila
02/25/07159Transplantation of Whole Kidney Marrow in Adult Zebrafish
02/25/07160Dissection of 6.5 dpc Mouse Embryos
02/25/07162Derivation of Hematopoietic Stem Cells from Murine Embryonic Stem Cells
02/25/07163Tracheotomy: A Method for Transplantation of Stem Cells to the Lung
02/25/07164Denaturing Gradient Gel Electrophoresis (DGGE)
02/17/07140Dissection of Imaginal Discs from 3rd Instar Drosophila Larvae
01/30/07134Growth Factor-Coated Bead Placement on Dorsal Forebrain Explants
01/30/07135Mouse Dorsal Forebrain Explant Isolation
01/05/07129Mouse Adrenal Chromaffin Cell Isolation
12/14/06127Testing Visual Sensitivity to the Speed and Direction of Motion in Lizards
11/30/06115Gibberella zeae Ascospore Production and Collection for Microarray Experiments.
11/30/06116Nuclear Transfer into Mouse Oocytes
11/30/06117Measuring Exocytosis in Neurons Using FM Labeling
11/30/06118ES Cell-derived Neuroepithelial Cell Cultures
11/30/06119Propagation of Human Embryonic Stem (ES) Cells
11/30/06120Long-term Imaging Mammalian Cells using Wide-Field Microscopy
11/30/06121Gene-gun Transfection of Hippocampal Neurons
11/20/06108Horizontal Slice Preparation of the Retina
11/13/06104Light/dark Transition Test for Mice
11/09/0684A Rapid Technique for the Visualization of Live Immobilized Yeast Cells
11/09/0685Dissection of Larval CNS in Drosophila Melanogaster
11/09/0686Human ES cells: Starting Culture from Frozen Cells
11/08/0664Assay for Adhesion and Agar Invasion in S. cerevisiae
11/08/0666Monitoring Actin Disassembly with Time-lapse Microscopy
10/19/0652Dissection of Drosophila Ovaries
10/12/0649Passaging HuES Human Embryonic Stem Cell-lines with Trypsin
10/12/0650Freezing Human ES Cells