- How Healthy Are You For Your Age?
May 22, 2013
- Biosensor That Detects Antibiotic Resistance Brings Us One Step Closer to Fighting Superbugs
May 8, 2013
- Jove Launches New Science Education Video Database
May 1, 2013
- Scientists Scan the Human Heart to Create Digital Anatomical Library
April 18, 2013
- Cell Phone Camera Photographs Microscopic Cell Samples
April 11, 2013
- JoVE Now Accepting Submissions For New Environmental Sciences Section
April 4, 2013
- Submissions Now Being Accepted For New Behavioral Sciences Section in JoVE
April 2, 2013
- Virtual Games Help the Blind Navigate Unknown Territory
March 27, 2013
- New Early Warning System for the Brain Development of Babies Published in Video Journal
March 14, 2013
- Origami Meets Chemistry in Scholarly Video-Article
February 13, 2013
Researchers Print Live Cells with a Standard Inkjet Printer
March 16, 2012
Researchers from Clemson University have found a way to create temporary holes in the membranes of live cells using a standard inkjet printer. The method will be published in JoVE, the Journal of Visualized Experiments, on March 16.
"We first had the idea for this method when we wanted to be able to visualize changes in the cytoskeleton arrangement due to applied forces on cells," said paper-author Dr. Delphine Dean.
She said other researchers have been using this method to print cells onto slides, but that they have only recently discovered that printing the cells causes the disruption in their membranes for a few hours. Creating temporary pores allow researchers to put molecules inside of cells that wouldn't otherwise fit, and study how the cells react.
"The authors have used an extremely innovative approach for bioprinting cells. Moreover, this approach can be used for applications other than cell printing," said JoVE Science Editor, Dr. Nandita Singh. "Matrix proteins can be printed onto substrates with this technique for cell patterning. This JoVE publication will make this approach simple and approachable and enable other labs to replicate the procedure."
The printer is modified by removing the paperfeed mechanism and adding a "stage" from which to feed the slides. The ink is replaced with a cell solution, and the cells are printed directly on to the slides.
Using this method, the researchers are able to process thousands of cells in a matter of minutes. Dr. Dean's team used the holes to introduce fluorescent molecules that illuminate the skeleton of the cell.
"We are actually interested in the cell mechanics of compressed cells. This method allows us to push on the cells and watch the response easily," said Dr. Dean. "We are interested in cardiovascular cells, and how they respond to mechanical force."
Dr. Dean chose to submit her method to JoVE, the only peer reviewed, PubMed-indexed science journal to publish all of its content in both text and video format, because, according to her, "until you've seen it done, it's hard to understand the process."
To watch the full video article, please click here: http://www.jove.com/video/3681/creating-transient-cell-membrane-pores-using-a-standard-inkjet-printer#
About JoVE, The Journal of Visualized Experiments:
JoVE, the Journal of Visualized Experiments, is the first and only PubMed/MEDLINE-indexed, peer-reviewed journal devoted to publishing scientific research in a video format. Using an international network of videographers, JoVE films and edits videos of researchers performing new experimental techniques at top universities, allowing students and scientists to learn them much more quickly. As of May 2013, JoVE has published video-protocols from an international community of more than 8,000 authors in the fields of biology, medicine, chemistry, and physics.
To link to this release, please use this link: http://www.jove.com/about/press-releases/24/researchers-print-live-cells-with-a-standard-inkjet-printer
Journal of Visualized Experiments
We offer complimentary access to verified press contacts. If you are interested in being on our press list, please create an account and send an email request to firstname.lastname@example.org.
Please make sure to follow our Twitter (http://www.twitter.com/JoVEJournal) account. If you have any questions or requests, contact us at email@example.com.