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Articles by Alba Guarné in JoVE

 JoVE Biology

Iterative Optimization of DNA Duplexes for Crystallization of SeqA-DNA Complexes

1Department of Biochemistry and Biomedical Sciences, McMaster University


JoVE 4266

Crystal structure of protein–DNA complexes can provide insight into protein function, mechanism, as well as, the nature of the specific interaction. Here, we report how to optimize the length, sequence and ends of duplex DNA for co-crystallization with Escherichia coli SeqA, a negative regulator of replication initiation.

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