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Articles by Alejandra Catalina Vélez-Ortega in JoVE

 JoVE Immunology and Infection

Generation of Induced Regulatory T Cells from Primary Human Naïve and Memory T Cells


JoVE 3738 4/16/2012

Department of Microbiology, Immunology and Molecular Genetics, University of Kentucky

We describe a method for generating regulatory, memory and naïve T cells from a single human blood donor. Polarized Tregs can be then compared to other subsets in a variety of genetic and functional applications with genetic homogeneity, including a suppression assay also detailed here.

Other articles by Alejandra Catalina Vélez-Ortega on PubMed

[Fasciola Hepatica Infestation: Infrequent Cause of Cholestasis]

[Constipation and Hypertransaminasemia As Presentation Form of Hypothyroidism]

[Cholestatic Hepatitis Caused by Gemfibrozil]

[Leishmaniasis in a Patient with Liver Cirrhosis]

We present a 35 years-old man with fever related leishmania infection as the first complication of a previously undiagnosed cirrhosis. Diagnosis of leishmaniasis is often complicated. Clinical and therapeutical aspects are commented.

[Interventional Radiology, Angioplasty and TIPS in Budd-Chiari Syndrome]

Budd-Chiari syndrome consists of complete or partial obstruction of the hepatic veins. Many treatment options are available; new interventional radiology techniques may play an important role as an alternative to surgical shunting.

Comparison of MELD, HVPG, and Their Changes to Predict Clinically Relevant Endpoints in Cirrhosis

Identification of predictors in the natural history of cirrhosis is based on determinations at a fixed time point. However, changes of these predictors may offer more information. To evaluate the predictive value of Model for End Stage Liver Disease (MELD) and hepatic venous pressure gradient (HVPG) and their changes in cirrhosis.

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