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In JoVE (1)
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Articles by Alexander J. Nedopil in JoVE
JoVE General
Implantação de Ferumoxides identificadas células-tronco mesenquimais em defeitos da cartilagem
Objetivo da apresentação é mostrar um método altamente reprodutível para gerar matriz associada implantes de células-tronco em defeitos da cartilagem, que pode ser visualizado com ressonância magnética. As células estaminais são rotulados com FDA-approved Ferumoxides, misturado com agarose, implantado em defeitos da cartilagem e fotografada com um scanner MR 7T.
Other articles by Alexander J. Nedopil on PubMed
An Optical Imaging Method to Monitor Stem Cell Migration in a Model of Immune-mediated Arthritis
The objective of this work is to establish an optical imaging technique that would enable monitoring of the integration of mesenchymal stem cells (MSC) in arthritic joints. Our approach is based on first developing a labeling technique of MSC with the fluorescent dye DiD followed by tracking the cell migration kinetics from the spatial distribution of the DiD fluorescence in optical images (OI). The experimental approach involves first the in vitro OI of MSC labeled with DiD accompanied by fluorescence microscopy measurements to establish localization of the signal within the cells. Thereafter, DiD-labeled MSC were injected into polyarthritic, athymic rats and the signal localization within the experimental animals was monitored over several days. The experimental results indicate that DiD integrated into the cell membrane. DiD-labeled MSC localization in the arthritic ankle joints was observed with OI indicating that this method can be applied to monitor MSC in arthritic joints.
Labeling Human Mesenchymal Stem Cells with Fluorescent Contrast Agents: the Biological Impact
This study aims to determine the effect of human mesenchymal stem cell (hMSC) labeling with the fluorescent dye DiD and the iron oxide nanoparticle ferucarbotran on chondrogenesis.
