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In JoVE (1)
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Articles by Alexey Y. Yukin in JoVE
Gene Delivery to Postnatal Rat Brain by Non-ventricular Plasmid Injection and Electroporation
Dmitry A. Molotkov1, Alexey Y. Yukin1,2, Ramil A. Afzalov1,2, Leonard S. Khiroug1
1Neuroscience Center, University of Helsinki, 2Faculty of Biological and Enviromental Sciences, University of Helsinki
This protocol describes a non-viral method of delivery of genetic constructs to a certain area of living rodent brain. The method consists of plasmid preparation, micropipette fabrication, neonatal rat pup surgery, microinjection of the construct, and in vivo electroporation.
Other articles by Alexey Y. Yukin on PubMed
Subplate Neurons Promote Spindle Bursts and Thalamocortical Patterning in the Neonatal Rat Somatosensory Cortex
The Journal of Neuroscience : the Official Journal of the Society for Neuroscience. Jan, 2012 | Pubmed ID: 22238105
Patterned neuronal activity such as spindle bursts in the neonatal cortex is likely to promote the maturation of cortical synapses and neuronal circuits. Previous work on cats has shown that removal of subplate neurons, a transient neuronal population in the immature cortex, prevents the functional maturation of thalamocortical and intracortical connectivity. Here we studied the effect of subplate removal in the neonatal rat primary somatosensory cortex (S1). Using intracortical EEG we show that after selective removal of subplate neurons in the limb region of S1, endogenous and sensory evoked spindle burst activity is largely abolished. Consistent with the reduced in vivo activity in the S1 limb region, we find by in vitro recordings that thalamocortical inputs to layer 4 neurons are weak. In addition, we find that removal of subplate neurons in the S1 barrel region prevents the development of the characteristic histological barrel-like appearance. Thus, subplate neurons are crucially involved in the generation of particular types of early network activity in the neonatal cortex, which are an important feature of cortical development. The altered EEG pattern following subplate damage could be applicable in the neurological assessment of human neonates.