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Articles by Alisa B. Schiffman in JoVE

 JoVE General

Detecção de imunofluorescência de dois análogos da timidina (CldU e UDI), em tecido primário


JoVE 2166 12/07/2010

Division of Endocrinology and Diabetes, Children’s Hospital of Philadelphia, Institute of Diabetes Obesity and Metabolism, Institute for Regenerative Medicine, Department of Pediatrics, University of Pennsylvania-School of Medicine

Temos uma estratégia de derivados para detectar incorporação seqüencial de análogos da timidina (CldU e UDI) em tecidos de camundongos adultos para quantificar duas rodadas sucessivas de divisão celular. Esta estratégia é útil para detectar renovação celular de longa duração tecidos, transformação oncogênicos, ou de trânsito ampliando-células.

Other articles by Alisa B. Schiffman on PubMed

Extremes of Clinical and Enzymatic Phenotypes in Children with Hyperinsulinism Caused by Glucokinase Activating Mutations

Heterozygous activating mutations of glucokinase have been reported to cause hypoglycemia attributable to hyperinsulinism in a limited number of families. We report three children with de novo glucokinase hyperinsulinism mutations who displayed a spectrum of clinical phenotypes corresponding to marked differences in enzyme kinetics.

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