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In JoVE (1)
Other Publications (5)
Articles by André Plagens in JoVE
JoVE General
Substrate Generation for Endonucleases of CRISPR/Cas Systems
Prokaryotic Small RNA Biology, Max-Planck-Institute for Terrestrial Microbiology
CRISPR/Cas systems mediate adaptive immunity in Bacteria and Archaea. Many Cas proteins are proposed to act as endoribonucleases acting on crRNA precursors of varying length. Here we illustrate three different approaches to generate pre-crRNA substrates for the biochemical analysis of Cas endonuclease activity.
Other articles by André Plagens on PubMed
The Impact of Onychomycosis on Quality of Life: Development of an International Onychomycosis-specific Questionnaire to Measure Patient Quality of Life
Onychomycosis is a widespread refractory disease deleteriously affecting quality of life via social stigma and disrupting daily activities. Many physicians perceive onychomycosis as a cosmetic rather than a medical problem.
Laterosubungual Giant Cell Tumor of the Tendon Sheath: an Unusual Location
Giant cell tumor of the tendon sheath is the second most frequent nonepithelial benign tumor of the hand after ganglion cyst. Although it is recognized as a condition that may involve the distal digit, there has been only 1 report of periungual involvement. We describe a second case at that site in this article.
Characterization of 463 Type I Markers Suitable for Dog Genome Mapping
In total, 463 canine gene markers were identified and characterized to serve as reagents in canine genome map projects. These markers are distributed over 221 canine gene markers, 139 TOASTs (Traced Orthologous Sequence Tags), 27 canine TOASTs, and 76 huESTs (human Expressed Sequence Tags). Out of 310 canine gene markers, 59%-84% were successfully amplified on dog DNA, the highest rates of success being observed when the exon/intron structure is known. Concerning TOASTs and human ESTs, of the 225 and 300 markers analyzed, 62% and 25% respectively were able to produce a dog positive amplification. As part of an ongoing project to map the canine genome using a dog/hamster radiation hybrid panel, these markers were tested for their specificity on dog versus hamster DNA. Thus 61%, 21%, and 12% of dog gene markers, TOASTs, and huESTs met the criteria required for radiation hybrid mapping, respectively. All of these 463 canine gene markers, however, are available and will be of value to any other mapping strategies.
Examining the Effect of Intervention to Nutritional Problems of Hospitalised Elderly: a Pilot Project
Up to 65% of elderly patients are protein-energy undernourished at admission or acquire nutritional deficits while hospitalised.
