Translate this page to:
In JoVE (1)
Other Publications (2)
Articles by Andrea Krühn in JoVE
Bacterial Delivery of RNAi Effectors: Transkingdom RNAi
Hermann Lage, Andrea Krühn
Institute of Pathology, Charité Campus Mitte
For development of RNA interference (RNAi)-based therapies, a novel strategy was developed, transkingdom RNAi (tkRNAi). This technology uses non-pathogenic bacteria to produce and deliver therapeutic short hairpin RNA (shRNA) into target cells. Here, tkRNAi was successfully applied for reversal of classical ABCB1-mediated multidrug resistance (MDR) of cancer cells.
Other articles by Andrea Krühn on PubMed
Journal of Molecular Spectroscopy. Dec, 1997 | Pubmed ID: 9446763
A newly recorded high-resolution infrared spectrum of hydrazine has been studied in the 729-1198 cm-1 region (the nu12 antisymmetric wagging band) with a resolution of 0.002 cm-1. About 1350 transitions with K' from 7 to 13 have been newly assigned and about 2350 transitions with lower values of K' reanalyzed with the improved precision. The effective parameters have been calculated separately for each value of K' using the Hougen-Ohashi hamiltonian for hydrazine. The extended assignment completes the analysis of the nu12 band of hydrazine. Copyright 1997 Academic Press. Copyright 1997Academic Press
Cancer Epidemiology, Biomarkers & Prevention : a Publication of the American Association for Cancer Research, Cosponsored by the American Society of Preventive Oncology. Feb, 2012 | Pubmed ID: 22337554
PURPOSE: Patients who receive a physician's recommendation for cancer screening and behavioral modification are most likely to comply with these recommendations. However, physicians face time constraints that make it nearly impossible to provide all recommended preventive services. The 2010 Affordable Care Act will expand health insurance coverage to 42 million Americans by 2014. Non-physician providers may help meet this new demand for primary care and ensure compliance with preventive services recommendations.METHODS: Data from the 2005 National Health Interview Survey were analyzed using multivariate logistic regression to assess the association between provider type seen in the past 12 months and compliance with U.S. Preventive Services Task Force cancer screening recommendations and receipt of behavior counseling among age-eligible adults (n = 23,201). Models were adjusted for age, level of education, and insurance status and stratified by gender.RESULTS: About 15% of NHIS participants (N = 4,652) saw a non-physician provider (nurse practitioner, certified nurse midwife or physician assistant) and a primary care physician. In adjusted analyses, age-eligible women were more likely to be compliant with Pap screening (OR: 5.0; 95% CI: 4.2-5.9), mammography (OR: 6.6; 95% CI: 5.2-8.4) and colorectal screening recommendations (OR: 7.8; 95% CI: 5.3-11.4) if they saw a non-physician provider and a primary care physician compared to not seeing any provider. Similarly, men were more likely to be compliant with colorectal screening recommendations (OR: 9.6; 95% CI: 6.9-13.5) if they saw a non-physician provider and a primary care physician. Women and men were more likely to report a provider asking about smoking status if they saw a non-physician provider and a primary care physician than those who saw other types of healthcare providers ((OR: 2.2; 95% CI: 2.0-2.4) and (OR: 3.0; 95% CI: 2.4-3.7), respectively).CONCLUSIONS: Seeing a non-physician provider and a primary care physician is related to an increased likelihood of compliance with cancer screening recommendations and receipt of health behavior counseling. Opportunities exist for non-physician providers during this era of healthcare reform. Cancer Epidemiol Biomarkers Prev; 21(3); 1-9. Â©2012 AACR.