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Articles by Angela L. Elwell in JoVE
10 nanogram Total RNA tek okuyun ve Eşleştirilmiş Sona mRNA-Seq Illumina Kütüphaneler
Srikumar Sengupta1, Jennifer M. Bolin1, Victor Ruotti1, Bao Kim Nguyen1, James A. Thomson1,2,3, Angela L. Elwell1, Ron Stewart1
1Regenerative Biology, Morgridge Institute for Research, 2Department of Cell & Regenerative Biology, University of Wisconsin, 3Department of Molecular, Cellular, & Regenerative Biology, University of California
Burada T7 lineer RNA amplifikasyon dayalı gen ekspresyon analizi için iki tek okudum ve eşleştirilmiş sonuna Illumina mRNA Seq sıralama kütüphaneler hazırlanması için bir yöntem açıklanmaktadır. Bu protokol, toplam RNA başlayan sadece 10 nanogram gerektirir ve tüm transkript temsil eden son derece tutarlı bir kütüphane oluşturur.
Other articles by Angela L. Elwell on PubMed
Separating Parental Environment from Seed Size Effects on Next Generation Growth and Development in Arabidopsis
Plant, Cell & Environment. Feb, 2011 | Pubmed ID: 20955226
Plant growth and development is profoundly influenced by environmental conditions that laboratory experimentation typically attempts to control. However, growth conditions are not uniform between or even within laboratories and the extent to which these differences influence plant growth and development is unknown. Experiments with wild-type Arabidopsis thaliana were designed to quantify the influences of parental environment and seed size on growth and development in the next generation. A single lot of seed was planted in six environmental chambers and grown to maturity. The seed produced was mechanically sieved into small and large size classes then grown in a common environment and subjected to a set of assays spanning the life cycle. Analysis of variance demonstrated that seed size effects were particularly significant early in development, affecting primary root growth and gravitropism, but also flowering time. Parental environment affected progeny germination time, flowering and weight of seed the progeny produced. In some cases, the parental environment affected the magnitude of (interacted with) the observed seed size effects. These data indicate that life history circumstances of the parental generation can affect growth and development throughout the life cycle of the next generation to an extent that should be considered when performing genetic studies.