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In JoVE (1)
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Articles by Ann-Marie Lawrence in JoVE
JoVE General
Окрашивание белков в гелях Кумасси G-250 без органических растворителей и уксусной кислоты
Protein Expression and Purification Core Facility, EMBL Heidelberg
Короткий протокол для белка окрашивание Кумасси бриллиантовый синий (CBB) G-250 в полиакриламидном геле описывается без использования органических растворителей или уксусной кислоты, как в классической процедуры окрашивания CBB.
Other articles by Ann-Marie Lawrence on PubMed
Simplified Screening for the Detection of Soluble Fusion Constructs Expressed in E. Coli Using a Modular Set of Vectors
The solubility of recombinant proteins expressed in bacteria is often disappointingly low. Several strategies have been developed to improve the yield and one of the most common strategies is the fusion of the target protein with a suitable partner. Despite several reports on the successful use of each of these carriers to increase the solubility of some recombinant proteins, none of them was always successful and a combinatorial approach seems more efficient to identify the optimal combination for a specific protein. Therefore, the efficiency of an expression system critically depends on the speed in the identification of the optimal combination for the suitable fusion candidate in a screening process. This paper describes a set of expression vectors (pETM) designed for rapid subcloning, expression and subsequent purification using immobilized metal affinity chromatography (IMAC).
