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In JoVE (1)
Other Publications (3)
Articles by Anne Béghin in JoVE
JoVE Bioengineering
Improved Visualization and Quantitative Analysis of Drug Effects Using Micropatterned Cells
1CYTOO Cell Architects, Grenoble, France, 2Centre Commun de Quantimétrie, Faculté de Médecine Rockefeller, Lyon, France
Adhesive micropatterns that normalize cellular architecture can be used to increase sensitivity in the detection of drug effects, improve reproducibility and simplify automated image acquisition and analysis. Such technology will benefit drug/siRNA screening assays, performed on conventional cell culture supports and consequently suffering from excessive cell-to-cell variability.
Other articles by Anne Béghin on PubMed
A Controlled Intervention Study on the Effects of a Very Rapidly Forward Rotating Shift System on Sleep-wakefulness and Well-being Among Young and Elderly Shift Workers
Shift work is related to problems in sleep/wakefulness and social life. The effects of a very rapidly forward rotating shift system on sleep, health and well-being of young (-45) and elderly (45+) maintenance workers were studied by a controlled intervention study. In the beginning, all the workers had a continuous backward rotating three-shift system. A very quickly forward rotating shift system was developed, avoiding consecutive night shifts and with more free-time between the individual shifts. The effect of the new shift system on sleep/wakefulness and general well-being was studied by questionnaire and field studies including on-site registration of sleep (actigraphy), subjective sleepiness (KSS) and psychomotor performance (PVT). Based on a linear mixed model for repeated measurements, the new shift system increased the main sleep length after the night shift and improved alertness and PVT performance during the night shift among the older workers. Alertness also improved during free-time after the night shift and sleep complaints decreased after all shifts. The workers on the new shift schedule perceived the effects of the new shift system on sleep, alertness, general health, well-being at work, social and family life more positively than the workers in the old shift system. At the end of the study, all subjects voted for the new shift system. It is concluded that although the new shift system increased the operating hours at night, the very rapidly forward rotating shift system had positive effects on the sleep, alertness and well-being of especially the older shift workers.
[Looking at Several Fleeting Hygiene Journals from the 19th Century Kept at the National Library of France]
The authors investigate some ephemeral reviews of private and public hygiene of the 19th century in the Bibliothèque nationale de France (BnF). They examine the general context of their publication, describe them according to the usual bibliographic criteria, analyse their aims and content, and try to understand why they were so ephemeral. These reviews are in a very poor state and computerisation, they hope, might give them a new life.
Phenotypic Spectrum of Fetal Smith-Lemli-Opitz Syndrome
The Smith-Lemli-Opitz syndrome (SLOS) is an autosomal recessive multiple congenital malformation syndrome caused by dehydrocholesterol reductase deficiency. The diagnosis is confirmed by high 7- and secondarily 8-dehydrocholesterol levels in plasma and tissues and/or by detection of biallelic mutations in the DHCR7 gene. The phenotypic spectrum of SLOS is broad, ranging from a mild phenotype combining subtle physical anomalies with behavioral and learning problems, to a perinatally lethal multiple malformations syndrome. The fetal phenotype of SLOS has been poorly described in the literature. We report a series of 10 fetuses with molecularly proven SLOS. Even in young fetuses, the facial dysmorphism appears characteristic. Genital abnormalities are rare in 46,XX subjects. Gonadal differentiation appears histologically normal and in agreement with the chromosomal sex, contrary to what has been previously stated. We observed some previously unreported anomalies: ulnar hypoplasia, vertebral segmentation anomalies, congenital pulmonary adenomatoid malformation, fused lungs, gastroschisis, holomyelia and hypothalamic hamartoma. This latter malformation proves that SLOS phenotypically overlaps with Pallister-Hall syndrome which remains clinically a major differential diagnosis of SLOS.
