Articles by Anthony J.A. Molina in JoVE

A Faster, High Resolution, mtPA-GFP-based Mitochondrial Fusion Assay Acquiring Kinetic Data of Multiple Cells in Parallel Using Confocal Microscopy

JoVE 3991 7/20/2012

Alenka Lovy¹, Anthony J.A. Molina², Fernanda M. Cerqueira³, Kyle Trudeau³, Orian S. Shirihai³

¹Department of Neuroscience, Center for Neuroscience Research, Tufts School of Medicine, ²Department of Internal Medicine, Geriatrics & Gerontology, Wake Forest Baptist Medical Center, ³Department of Medicine, Boston University Medical Center

Mitochondrial fusion was measured by tracking the equilibration of photoconverted matrix-targeted GFP across the mitochondrial network over time. Thus far, only one cell could be subjected to an hour long kinetic analysis at a time. We present a method that simultaneously measures multiple cells, thereby speeding up the data collection process.