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In JoVE (1)
Other Publications (3)
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Articles by Atefeh Ghogha in JoVE
Induktion Dendritic Tillväxten i Odlade sympatiska nervceller
Atefeh Ghogha, Donald A. Bruun, Pamela J. Lein
Department of Molecular Biosciences, University of California, Davis
Vi beskriver ett protokoll för användning av benmorfogenetiskt protein-7 (BMP-7) eller Matrigel att selektivt inducera dendritisk tillväxt i primära sympatiska neuroner dissocierade från ganglion cervicale superius (SCG) hos perinatal råtta.
Other articles by Atefeh Ghogha on PubMed
Molecular and Cellular Biology. Apr, 2008 | Pubmed ID: 18212043
The chemotropic guidance cue netrin-1 promotes neurite outgrowth through its receptor Deleted in Colorectal Cancer (DCC) via activation of Rac1. The guanine nucleotide exchange factor (GEF) linking netrin-1/DCC to Rac1 activation has not yet been identified. Here, we show that the RhoGEF Trio mediates Rac1 activation in netrin-1 signaling. We found that Trio interacts with the netrin-1 receptor DCC in mouse embryonic brains and that netrin-1-induced Rac1 activation in brain is impaired in the absence of Trio. Trio(-/-) cortical neurons fail to extend neurites in response to netrin-1, while they are able to respond to glutamate. Accordingly, netrin-1-induced commissural axon outgrowth is reduced in Trio(-/-) spinal cord explants, and the guidance of commissural axons toward the floor plate is affected by the absence of Trio. The anterior commissure is absent in Trio-null embryos, and netrin-1/DCC-dependent axonal projections that form the internal capsule and the corpus callosum are defective in the mutants. Taken together, these findings establish Trio as a GEF that mediates netrin-1 signaling in axon outgrowth and guidance through its ability to activate Rac1.
Para- and Ortho-substitutions Are Key Determinants of Polybrominated Diphenyl Ether Activity Toward Ryanodine Receptors and Neurotoxicity
Environmental Health Perspectives. Apr, 2011 | Pubmed ID: 21106467
Polybrominated diphenyl ethers (PBDEs) are widely used flame retardants that bioaccumulate in human tissues. Their neurotoxicity involves dysregulation of calcium ion (Ca(2+))signaling; however, specific mechanisms have yet to be defined.
The Activation of Ezrin-radixin-moesin Proteins is Regulated by Netrin-1 Through Src Kinase and RhoA/Rho Kinase Activities and Mediates Netrin-1-induced Axon Outgrowth
Molecular Biology of the Cell. Oct, 2011 | Pubmed ID: 21849478
The receptor Deleted in Colorectal Cancer (DCC) mediates the attractive response of axons to the guidance cue netrin-1 during development. On netrin-1 stimulation, DCC is phosphorylated and induces the assembly of signaling complexes within the growth cone, leading to activation of cytoskeleton regulators, namely the GTPases Rac1 and Cdc42. The molecular mechanisms that link netrin-1/DCC to the actin machinery remain unclear. In this study we seek to demonstrate that the actin-binding proteins ezrin-radixin-moesin (ERM) are effectors of netrin-1/DCC signaling in embryonic cortical neurons. We show that ezrin associates with DCC in a netrin-1-dependent manner. We demonstrate that netrin-1/DCC induces ERM phosphorylation and activation and that the phosphorylation of DCC is required in that context. Moreover, Src kinases and RhoA/Rho kinase activities mediate netrin-1-induced ERM phosphorylation in neurons. We also observed that phosphorylated ERM proteins accumulate in growth cone filopodia, where they colocalize with DCC upon netrin-1 stimulation. Finally, we show that loss of ezrin expression in cortical neurons significantly decreases axon outgrowth induced by netrin-1. Together, our findings demonstrate that netrin-1 induces the formation of an activated ERM/DCC complex in growth cone filopodia, which is required for netrin-1-dependent cortical axon outgrowth.