Articles by Bivian Torres in JoVE

Flow Cytometry Purification of Mouse Meiotic Cells

JoVE 2602 4/15/2011

Irina V. Getun¹, Bivian Torres², Philippe R.J. Bois¹

¹Genome Plasticity Laboratory, Department of Cancer Biology, The Scripps Research Institute, ²Flow Cytometry Core, The Scripps Research Institute

An efficient method to obtain highly purified viable meiotic fractions from mouse testis is described, which combines a refined cell dissociation protocol with fluorescent activated cell sorting (FACS). This method takes advantage of differences in the DNA content and nuclear density of discrete meiotic fractions.