Articles by Bonnie L. Chaban in JoVE

Multiplex Detection of Bacteria in Complex Clinical and Environmental Samples using Oligonucleotide-coupled Fluorescent Microspheres

JoVE 3344 10/23/2011

Tim J. Dumonceaux¹, Jennifer R. Town¹, Janet E. Hill², Bonnie L. Chaban², Sean M. Hemmingsen³

¹Saskatoon Research Centre, Agriculture and Agri-Food Canada, ²Department of Veterinary Microbiology, University of Saskatchewan, ³Plant Biotechnology Institute, National Research Council of Canada

We describe a multiplex method for the detection of microorganisms within a sample using oligonucleotide-coupled fluorescent beads. Amplicon from all organisms within a sample is hybridized to a panel of probe-coupled beads. A Luminex or Bio-Plex instrument is used to query each bead for bead type and hybridization signal.