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In JoVE (1)
Other Publications (1)
Articles by Bryan Fiema in JoVE
JoVE Clinical and Translational Medicine
High Throughput Sequential ELISA for Validation of Biomarkers of Acute Graft-Versus-Host Disease
Pediatric Blood and Marrow Transplant Program, University of Michigan
High throughput validation of multiple candidate biomarkers can be performed by sequential ELISA in order to minimize freeze/thaw cycles and use of precious plasma samples. Here, we demonstrate how to sequentially perform ELISAs for six different validated plasma biomarkers1-3 of graft-versus-host disease (GVHD)4 on the same plasma sample.
Other articles by Bryan Fiema on PubMed
The Effect of Light and Glucose Concentration on the Content of S- and R-glucans in the Mycelium of Aspergillus Giganteus Mut. Alba
The amounts of S- and R-glucans in the mycelium of Aspergillus giganteus mut. alba depend on light within the range of selected light intensities: 0 W.m-2; 3 W.m-2; 10.6 W.m-2 at 20% glucose concentration in the nutrient medium. This species synthesizes the highest amount of S-glucans at the light intensity 10.6 W.m-2, and that of R-glucans--at the intensity 3 W.m-2.
