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In JoVE (1)

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Articles by Bryan Fiema in JoVE

 JoVE Medicine

High Throughput Sequential ELISA for Validation of Biomarkers of Acute Graft-Versus-Host Disease

1Pediatric Blood and Marrow Transplant Program, University of Michigan


JoVE 4247

High throughput validation of multiple candidate biomarkers can be performed by sequential ELISA in order to minimize freeze/thaw cycles and use of precious plasma samples. Here, we demonstrate how to sequentially perform ELISAs for six different validated plasma biomarkers1-3 of graft-versus-host disease (GVHD)4 on the same plasma sample.

Other articles by Bryan Fiema on PubMed

The Effect of Light and Glucose Concentration on the Content of S- and R-glucans in the Mycelium of Aspergillus Giganteus Mut. Alba

The amounts of S- and R-glucans in the mycelium of Aspergillus giganteus mut. alba depend on light within the range of selected light intensities: 0 W.m-2; 3 W.m-2; 10.6 W.m-2 at 20% glucose concentration in the nutrient medium. This species synthesizes the highest amount of S-glucans at the light intensity 10.6 W.m-2, and that of R-glucans--at the intensity 3 W.m-2.

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