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In JoVE (1)
Other Publications (1)
Articles by Bryan Fiema in JoVE
High Throughput Sequential ELISA for Validation of Biomarkers of Acute Graft-Versus-Host Disease
Bryan Fiema*1, Andrew C. Harris*1, Aurelie Gomez1, Praechompoo Pongtornpipat1, Kelly Lamiman1, Mark T. Vander Lugt1, Sophie Paczesny1
1Pediatric Blood and Marrow Transplant Program, University of Michigan
High throughput validation of multiple candidate biomarkers can be performed by sequential ELISA in order to minimize freeze/thaw cycles and use of precious plasma samples. Here, we demonstrate how to sequentially perform ELISAs for six different validated plasma biomarkers1-3 of graft-versus-host disease (GVHD)4 on the same plasma sample.
Published October 31, 2012. Keywords: Medicine, ELISA, Sequential ELISA, Cytokine, Blood plasma, biomarkers, proteomics, graft-versus-host disease, Small sample, Quantification
Other articles by Bryan Fiema on PubMed
The Effect of Light and Glucose Concentration on the Content of S- and R-glucans in the Mycelium of Aspergillus Giganteus Mut. Alba
Journal of Basic Microbiology. 1993 | Pubmed ID: 8478791
The amounts of S- and R-glucans in the mycelium of Aspergillus giganteus mut. alba depend on light within the range of selected light intensities: 0 W.m-2; 3 W.m-2; 10.6 W.m-2 at 20% glucose concentration in the nutrient medium. This species synthesizes the highest amount of S-glucans at the light intensity 10.6 W.m-2, and that of R-glucans--at the intensity 3 W.m-2.