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In JoVE (1)
Other Publications (1)
Articles by Caragh Whitehead in JoVE
JoVE General
High-throughput Saccharification Assay for Lignocellulosic Materials
Center for Novel Agricultural Products, University of York
A simple, rapid method for determining the saccharification potential of large numbers of plant biomass samples is described. The automated platform for this analysis involves the preparation of the plant biomass for analysis in 96 well plates and the subsequent performance of pretreatment, hydrolysis and quantification of the sugars released.
Other articles by Caragh Whitehead on PubMed
Automated Saccharification Assay for Determination of Digestibility in Plant Materials
Cell wall resistance represents the main barrier for the production of second generation biofuels. The deconstruction of lignocellulose can provide sugars for the production of fuels or other industrial products through fermentation. Understanding the biochemical basis of the recalcitrance of cell walls to digestion will allow development of more effective and cost efficient ways to produce sugars from biomass. One approach is to identify plant genes that play a role in biomass recalcitrance, using association genetics. Such an approach requires a robust and reliable high throughput (HT) assay for biomass digestibility, which can be used to screen the large numbers of samples involved in such studies.
