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In JoVE (1)
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Articles by Carlos Garcia in JoVE
JoVE General
A conversão de um ELISA de captura para um ensaio de xMAP Luminex usando um método de rastreio de anticorpos Multiplex
1Chemistry Research and Development, Luminex Corporation, 2Global Marketing, Luminex Corporation
Um ELISA pode ser facilmente convertido para um ensaio de xMAP Luminex e, através dos benefícios da multiplexação, vários anticorpos podem ser rastreados simultaneamente para identificar um par de anticorpos óptima, resultando em aumento da sensibilidade e gama dinâmica, enquanto reduz o custo do ensaio.
Other articles by Carlos Garcia on PubMed
Nutrient Metal Sequestration by Calprotectin Inhibits Bacterial Superoxide Defense, Enhancing Neutrophil Killing of Staphylococcus Aureus
By sequestering manganese and zinc, the neutrophil protein calprotectin plays a crucial role in host defense against bacterial and fungal pathogens. However, the essential processes disrupted by calprotectin remain unknown. We report that calprotectin enhances the sensitivity of Staphylococcus aureus to superoxide through inhibition of manganese-dependent bacterial superoxide defenses, thereby increasing superoxide levels within the bacterial cell. Superoxide dismutase activity is required for full virulence in a systemic model of S. aureus infection, and disruption of staphylococcal superoxide defenses by calprotectin augments the antimicrobial activity of neutrophils promoting in vivo clearance. Calprotectin mutated in two transition metal binding sites and therefore defective in binding manganese and zinc does not inhibit microbial growth, unequivocally linking the antimicrobial properties of calprotectin to metal chelation. These results suggest that calprotectin contributes to host defense by rendering bacterial pathogens more sensitive to host immune effectors and reducing bacterial growth.
