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In JoVE (1)
Other Publications (1)
Articles by Chuen Lo in JoVE
Fabrication of Micro-tissues using Modules of Collagen Gel Containing Cells
M. Dean Chamberlain1, Mark J. Butler1, Ema C. Ciucurel1, Lindsay E. Fitzpatrick2, Omar F. Khan1, Brendan M. Leung2, Chuen Lo1, Ritesh Patel2, Alexandra Velchinskaya2, Derek N. Voice2, Michael V. Sefton1
1Institute of Biomaterials and Biomedical Engineering / Department of Chemical Engineering and Applied Chemistry, University of Toronto, 2Institute of Biomaterials and Biomedical Engineering, University of Toronto
Creation of micro-tissues using cylindrical collagen gels, called modules, that contain embedded cells and which surface is coated with endothelial cells.
Other articles by Chuen Lo on PubMed
Effect of Mouse VEGF164 on the Viability of Hydroxyethyl Methacrylate-methyl Methacrylate-microencapsulated Cells in Vivo: Bioluminescence Imaging
Journal of Biomedical Materials Research. Part A. Nov, 2008 | Pubmed ID: 18181105
Bioluminescent imaging was used to track the viability of luciferase transfected L929 cells in poly(hydroxyethyl methacrylate-co-methyl methacrylate) (HEMA-MMA) microcapsules. Bioluminescence, as determined by Xenogen imaging after addition of luciferin to microcapsules in vitro, increased with time, consistent with an increase in cell number. Capsules were suspended in Matrigel and injected subcutaneously. The bioluminesence in vivo increased over the first 3 weeks and then decreased, both with and without the delivery of mVEGF(164) (1.2 ng/24 h/200 microcapsules in vitro); VEGF delivery was from microencapsulated doubly transfected cells (both luciferase and mVEGF(164)). VEGF delivery was sufficient to generate a greater number of vascular structures, but this did not result in the expected increase in microencapsulated cell viability. Interestingly, the number of vessels at day 28 was less than at day 21, consistent with what would be an expected reduction in VEGF secretion when cell viability is lost. The results presented here do not support the hypothesis that transfection of microencapsulated cells with VEGF is sufficient to correct the oxygen transport limitation, at least with this type of tissue engineering construct. On the other hand, bioluminescent imaging proved to be a useful method of monitoring microencapsulated cell viability over many weeks in vivo.