Articles by Chun-Ming Huang in JoVE

Sampling Human Indigenous Saliva Peptidome Using a Lollipop-Like Ultrafiltration Probe: Simplify and Enhance Peptide Detection for Clinical Mass Spectrometry

JoVE 4108 8/07/2012

Wenhong Zhu¹, Richard L. Gallo^2,3, Chun-Ming Huang^2,3,4

¹Sanford-Burnham Medical Research Institute, ²Division of Dermatology, University of California, San Diego, ³VA San Diego Healthcare Center, ⁴Moores Cancer Center, University of California, San Diego

Considering saliva sampling for future clinical application, a lollipop-like ultrafiltration (LLUF) probe was fabricated to fit in the human oral cavity. Direct analysis of undigested saliva by NanoLC-LTQ mass spectrometry demonstrated the ability of LLUF probes to remove large proteins and high abundance proteins, and make low-abundant peptides more detectable.