Articles by Claudia Rodler in JoVE

Oral Biofilm Analysis of Palatal Expanders by Fluorescence In-Situ Hybridization and Confocal Laser Scanning Microscopy

JoVE 2967 10/20/2011

Barbara Klug^1,2, Claudia Rodler¹, Martin Koller³, Gernot Wimmer³, Harald H. Kessler², Martin Grube⁴, Elisabeth Santigli¹

¹Department of Orthodontics and Maxillofacial Orthopedics, Medical University of Graz, ²Institute of Hygiene, Microbiology and Environmental Medicine, Medical University of Graz, ³Department of Prosthodontics, Restorative Dentistry, Periodontology and Implantology, Medical University of Graz, ⁴Institute of Plant Sciences, Karl-Franzens-University Graz

We present a protocol for structural and compositional analysis of natural oral biofilm from orthodontic appliances with in situ hybridization (FISH) and confocal laser scanning microscopy (CLSM). Oral biofilm samples were collected from palatal expanders, scraping acrylic-resin flakes off their surface and referring them for molecular processing.