Articles by Constantin Nelep in JoVE

Improved Visualization and Quantitative Analysis of Drug Effects Using Micropatterned Cells

JoVE 2514 12/02/2010

Sébastien Degot¹, Muriel Auzan¹, Violaine Chapuis¹, Anne Béghin², Amélie Chadeyras¹, Constantin Nelep¹, Maria Luisa Calvo-Muñoz¹, Joanne Young¹, François Chatelain¹, Alexandra Fuchs¹

¹CYTOO Cell Architects, Grenoble, France, ²Centre Commun de Quantimétrie, Faculté de Médecine Rockefeller, Lyon, France

Adhesive micropatterns that normalize cellular architecture can be used to increase sensitivity in the detection of drug effects, improve reproducibility and simplify automated image acquisition and analysis. Such technology will benefit drug/siRNA screening assays, performed on conventional cell culture supports and consequently suffering from excessive cell-to-cell variability.