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Articles by Danilo Maddalo in JoVE
JoVE General
Intracellulære hvorved man genfolder Assay
Institute of Toxicology and Genetics, Karlsruhe Institute of Technology
I denne protokol en metode til at måle intracellulært protein, hvorved man genfolder efter heat shock er beskrevet. Denne metode kan bruges til at studere foldases som molekylær anstandsdamer og deres co-faktorer eller stoffer i stand til at påvirke deres aktivitet. Firefly luciferase aktiviteten bruges som reporter til at måle anstandsdame hvorved man genfolder aktivitet.
Other articles by Danilo Maddalo on PubMed
The Anterior Gradient 2 (AGR2) Gene is Overexpressed in Prostate Cancer and May Be Useful As a Urine Sediment Marker for Prostate Cancer Detection
AGR2 is a member of the endoplasmatic reticulum protein disulphide isomerase gene family implicated in tumor metastasis. Its expression pattern, function, and utility as a marker remains to be further investigated.
