Articles by David W. Ehrhardt in JoVE

Time-lapse Fluorescence Imaging of Arabidopsis Root Growth with Rapid Manipulation of The Root Environment Using The RootChip

JoVE 4290 7/07/2012

Guido Grossmann¹, Matthias Meier^2,3,4, Heather N. Cartwright¹, Davide Sosso¹, Stephen R. Quake^2,3, David W. Ehrhardt¹, Wolf B. Frommer¹

¹Department of Plant Biology, Carnegie Institution for Science, ²Howard Hughes Medical Institute, ³Departments of Applied Physics and Bioengineering, Stanford University, ⁴Department of Microsystems Engineering (IMTEK) and Center for Biological Signaling Studies (BIOSS), University of Freiburg

This article provides a protocol for cultivation of Arabidopsis seedlings in the RootChip, a microfluidic imaging platform that combines automated control of growth conditions with microscopic root monitoring and FRET-based measurement of intracellular metabolite levels.