Articles by David Svilar in JoVE

Quantitative, Real-time Analysis of Base Excision Repair Activity in Cell Lysates Utilizing Lesion-specific Molecular Beacons

JoVE 4168 8/06/2012

David Svilar^1,2, Conchita Vens³, Robert W. Sobol^1,2,4

¹Department of Pharmacology & Chemical Biology, University of Pittsburgh School of Medicine, ²Hillman Cancer Center, University of Pittsburgh Cancer Institute, ³Department of Experimental Therapy, The Netherlands Cancer Institute, ⁴Department of Human Genetics, University of Pittsburgh School of Public Health

We describe a method for the quantitative, real-time measurement of DNA glycosylase and AP endonuclease activities in cell nuclear lysates. The assay yields rates of DNA Repair activity amenable to kinetic analysis and is adaptable for quantification of DNA Repair activity in tissue and tumor lysates or with purified proteins.