JoVE General
Melanie Jungblut, Karen Oeltze, Irene Zehnter, Doris Hasselmann, Andreas Bosio
Miltenyi Biotec,GmbH
This video describes a simple, time-saving technique for automated tissue dissociation using the gentleMACS Dissociator to prepare single-cell suspensions of mouse splenocytes.
JoVE General
Melanie Jungblut, Karen Oeltze, Irene Zehnter, Doris Hasselmann, Andreas Bosio
Miltenyi Biotec,GmbH
Dissociating cells from specific tissue types requires specific parameters for tissue agitation to obtain a high volume of viable, culturable cells. The Miltenyi gentleMACS dissociator optimizes this task with a simple, practical protocol. In this publication the use of this apparatus on lung tissue is explained.
JoVE General
Sandra Pennartz, Sandy Reiss, Rebecca Biloune, Doris Hasselmann, Andreas Bosio
Miltenyi Biotec,GmbH
Dissociating cells from specific tissue types requires specific parameters for tissue aggitation to obtain a high volume of viable, culturable cells. The Miltenyi gentleMACS Dissociator optimizes this task with a simple, practical protocol. In this publication the use of this apparatus on nerual tissue is explained.