Articles by DuHyun Jun in JoVE

Isolation & Characterization of Hoechst^low CD45^negative Mouse Lung Mesenchymal Stem Cells

JoVE 3159 10/26/2011

Kelsey S. Chow^1,2, DuHyun Jun^1,2, Karen M. Helm³, David H. Wagner^1,2,4, Susan M. Majka^1,2,3

¹Charles C. Gates Regenerative Medicine and Stem Cell Biology Program, University of Colorado Denver, ²Department of Medicine, University of Colorado Denver, ³Cancer Center, University of Colorado Denver, ⁴Webb Waring Institute, University of Colorado Denver

In this article we demonstrate the isolation of murine resident lung mesenchymal stem cells (lung MSC), their expansion, characterization and analysis of immunomodulatory properties.