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In JoVE (2)
- स्तन IVIS स्पेक्ट्रम का उपयोग ट्यूमर के vivo bioluminescent इमेजिंग में
- Bioluminescence और माइक्रो सीटी इमेजिंग के साथ ट्यूमर Metastases और osteolytic घावों की निगरानी
Other Publications (7)
- Surgery
- Cancer Research
- Conference Proceedings : ... Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Conference
- Clinical Cancer Research : an Official Journal of the American Association for Cancer Research
- Analytical Chemistry
- Annals of the Academy of Medicine, Singapore
- PloS One
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Articles by Ed Lim in JoVE
JoVE General
स्तन IVIS स्पेक्ट्रम का उपयोग ट्यूमर के vivo bioluminescent इमेजिंग में
Biology Research and Development , Caliper Life Sciences
स्तन ट्यूमर कोशिकाओं luciferase व्यक्त subcutaneously चूहों में प्रत्यारोपित कर रहे हैं और कल्पना का उपयोग करने के लिए ऑप्टिकल इमेजिंग ट्यूमर और गैर invasively एक अनुदैर्ध्य अध्ययन में वृद्धि और विकास पर नजर रखने के.
JoVE Clinical and Translational Medicine
Bioluminescence और माइक्रो सीटी इमेजिंग के साथ ट्यूमर Metastases और osteolytic घावों की निगरानी
Imaging Biology Research and Development, Caliper Life Sciences
अस्थि मेटास्टेसिस के एक प्रयोगात्मक माउस मॉडल luciferase स्तन ट्यूमर कोशिकाओं को व्यक्त करने के intracardiac प्रसव के बाद स्थापित किया गया था. ट्यूमर विकास और osteolytic घाव परिणामस्वरूप longitudinally bioluminescence और सूक्ष्म सीटी इमेजिंग के साथ निगरानी की गई.
Other articles by Ed Lim on PubMed
Pelvic Fracture in the Elderly is Associated with Increased Mortality
The elderly population is currently the fastest growing sector in America. The purpose of this study was to examine the age-related outcome in patients after blunt pelvic injury.
Noninvasive Bioluminescence Imaging of Normal and Spontaneously Transformed Prostate Tissue in Mice
Several transgenic mouse models of prostate cancer have been developed recently that are able to recapitulate many key biological features of the human condition. It would, therefore, be desirable to employ these models to test the efficacy of new therapeutics before clinical trial; however, the variable onset and non-visible nature of prostate tumor development limit their use for such applications. We now report the generation of a transgenic reporter mouse that should obviate these limitations by enabling noninvasive in vivo bioluminescence imaging of normal and spontaneously transformed prostate tissue in the mouse. We used an 11-kb fragment of the human prostate-specific antigen (PSA) promoter to achieve specific and robust expression of firefly luciferase in the prostate glands of transgenic mice. Ex vivo bioluminescence imaging and in situ hybridization analysis confirmed that luciferase expression was restricted to the epithelium in all four lobes of the prostate. We also show that PSA-Luc mice exhibit decreased but readily detectable levels of in vivo bioluminescence over extended time periods following androgen ablation. These results suggest that this reporter should enable in vivo imaging of both androgen-dependent and androgen-independent prostate tumor models. As proof-of-principle, we show that we could noninvasively image SV40 T antigen-induced prostate tumorigenesis in mice with PSA-Luc. Furthermore, we show that our noninvasive imaging strategy can be successfully used to image tumor response to androgen ablation in transgenic mice and, as a result, that we can rapidly identify individual animals capable of sustaining tumor growth in the absence of androgen.
A Novel Image Capture System for Use in Telehealth Applications
A novel image capture and retrieval system has been developed for use in a range of telehealth applications in the home and in residential care facilities. The system is based around the JPEG 2000 standard and uses the PTP protocol for image capture from any high resolution digital camera and the Kakadu suite of JPEG2000 utilities to serve the collected images via a proxy server over any available communication channel from telephone lines to broadband services. When coupled with an image processing system such as the AMWIS system for pressure wound management, the system provides a high level of clinical functionality suitable for a wide range of telemedicine applications in rural and remote sites.
A Spontaneous Acinar Cell Carcinoma Model for Monitoring Progression of Pancreatic Lesions and Response to Treatment Through Noninvasive Bioluminescence Imaging
We have generated an EL1-luc/TAg transgenic mouse model that develops spontaneous and bioluminescent acinar cell carcinomas. We applied this model to noninvasively monitor tumor development and drug response.
Fluorescent Nano-optodes for Glucose Detection
We have designed fluorescent nanosensors based on ion-selective optodes capable of detecting small molecules. By localizing the sensor components in a hydrophobic core, these nanosensors are able to monitor dynamic changes in concentration of the model analyte, glucose. The nanosensors demonstrated this response in vitro and also when injected subcutaneously into mice. The response of the nanosensors tracked changes in blood glucose levels in vivo that were comparable to measurements taken using a glucometer. The development of these nanosensors offers an alternative, minimally invasive tool for monitoring glucose levels in such fields as diabetes research. Furthermore, the extension of the ion-selective optode sensor platform to small molecule detection will allow for enhanced monitoring of physiological processes.
Enhancement of a Master-slave Robotic System for Natural Orifice Transluminal Endoscopic Surgery
Introduction: A novel robotic platform for Natural Orifice Transluminal Endoscopic Surgery (NOTES) is presented in this paper. It aims to tackle two crucial technical barriers which hinder its smooth transition from animal studies to clinical trials: providing effective instrumentations to perform complex NOTES procedures and maintaining the spatial orientation for endoscopic navigation. Materials and Methods: The technical barriers are overcome by the design of the robotic system considering size, triangulation, dexterity, maneuverability and complexity. It is also shown that haptic feedback and interventional navigation system could solve the problem of off-axis manipulation of the camera angle and loss of spatial orientation upon entering the peritoneal cavity in transgastric NOTES procedure, respectively. Results: Successful ESD (endoscopic submucosal dissection) and wedge hepatic resection have been performed on live pigs with our Master And Slave Transluminal Endoscopic Robot (MASTER) system, showing its capability to perform advanced endoscopic surgical and NOTES procedures. It is found that the MASTER exhibited good grasping and cutting efficiency. And the lesion resection time could be significantly reduced with more practice between the endoscopist and the robot operator. Conclusion: This study evaluates the feasibility of MASTER system as a platform overcoming the barriers to NOTES. It is also demonstrated that the MASTER could effectively mitigate the technical constraints normally encountered in NOTES procedures.
Imaging Pulmonary NF-kappaB Activation and Therapeutic Effects of MLN120B and TDZD-8
NF-κB activation is a critical signaling event in the inflammatory response and has been implicated in a number of pathological lung diseases. To enable the assessment of NF-κB activity in the lungs, we transfected a luciferase based NF-κB reporter into the lungs of mice or into Raw264.7 cells in culture. The transfected mice showed specific luciferase expression in the pulmonary tissues. Using these mouse models, we studied the kinetics of NF-κB activation following exposure to lipopolysaccharide (LPS). The Raw264.7 cells expressed a dose-dependent increase in luciferase following exposure to LPS and the NF-κB reporter mice expressed luciferase in the lungs following LPS challenge, establishing that bioluminescence imaging provides adequate sensitivity for tracking the NF-κB activation pathway. Interventions affecting the NF-κB pathway are promising clinical therapeutics, thus we further examined the effect of IKK-2 inhibition by MLN120B and glycogen synthase kinase 3 beta inhibition by TDZD-8 on NF-κB activation. Pre-treatment with either MLN120B or TDZD-8 attenuated NF-κB activation in the pulmonary tissues, which was accompanied with suppression of pro-inflammatory chemokine MIP-1ß and induction of anti-inflammatory cytokine IL-10. In summary, we have established an imaging based approach for non-invasive and longitudinal assessment of NF-κB activation and regulation during acute lung injury. This approach will potentiate further studies on NF-κB regulation under various inflammatory conditions.
