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JoVE Neuroscience Ex vivo Culturing of Whole, Developing Drosophila Brains JoVE 4270 7/27/2012 Ranjini Prithviraj1,2, Svetlana Trunova1,2, Edward Giniger1,2 1National Institute of Neurological Disorders and Stroke, 2National Human Genome Research Institute, National Institutes of Health, Bethesda, MD This article describes a method by which one can mimic in vivo development of the Drosophila mushroom body in an ex vivo culture system.