Articles by Elizabeth Normand in JoVE

A Practical Approach to Genetic Inducible Fate Mapping: A Visual Guide to Mark and Track Cells In Vivo

JoVE 1687 12/30/2009

Ashly Brown¹, Stephen Brown², Debra Ellisor², Nellwyn Hagan¹, Elizabeth Normand¹, Mark Zervas²

¹Department of Neuroscience, Division of Biology and Medicine, Brown University, ²Department of Molecular Biology, Cell Biology and Biochemistry, Division of Biology and Medicine, Brown University

Genetic Inducible Fate Mapping (GIFM) marks and tracks cells with fine spatial and temporal control in vivo and elucidates how cells from a specific genetic lineage contribute to developing and adult tissues. Demonstrated here are the techniques required to fate map E12.5 mouse embryos for epifluorescent and explant analysis.