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In JoVE (1)
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Articles by Emiri T. Mandeville in JoVE
Cerebral blodets syresättning Mätning baseras på syre-beroende härdning av Fosforescens
Sava Sakadžić1, Emmanuel Roussakis2, Mohammad A. Yaseen1, Emiri T. Mandeville3, Vivek J. Srinivasan1, Ken Arai3, Svetlana Ruvinskaya1, Weicheng Wu1, Anna Devor1,4, Eng H. Lo3, Sergei A. Vinogradov2, David A. Boas1
1Optics Division, Athinoula A. Martinos Center for Biomedical Imaging, Department of Radiology, Massachusetts General Hospital and Harvard Medical School, 2Department of Biochemistry and Biophysics, University of Pennsylvania, 3Neuroprotection Research Laboratory, Departments of Radiology and Neurology, Massachusetts General Hospital and Harvard Medical School, 4Departments of Neurosciences and Radiology, University of California
Vi presenterar en experimentell metoder för att mäta partialtrycket av syre (PO2) i hjärnans kärlsystem baseras på syre-beroende härdning av fosforescens. Animal förberedelser och procedurer bildhantering angavs för både stora synfält CCD-baserad avbildning av pO2 på råttor och 2-photon excitation baserad avbildning av pO2 i möss.
Other articles by Emiri T. Mandeville on PubMed
Two-photon High-resolution Measurement of Partial Pressure of Oxygen in Cerebral Vasculature and Tissue
Nature Methods. Sep, 2010 | Pubmed ID: 20693997
Measurements of oxygen partial pressure (pO(2)) with high temporal and spatial resolution in three dimensions is crucial for understanding oxygen delivery and consumption in normal and diseased brain. Among existing pO(2) measurement methods, phosphorescence quenching is optimally suited for the task. However, previous attempts to couple phosphorescence with two-photon laser scanning microscopy have faced substantial difficulties because of extremely low two-photon absorption cross-sections of conventional phosphorescent probes. Here we report to our knowledge the first practical in vivo two-photon high-resolution pO(2) measurements in small rodents' cortical microvasculature and tissue, made possible by combining an optimized imaging system with a two-photon-enhanced phosphorescent nanoprobe. The method features a measurement depth of up to 250 microm, sub-second temporal resolution and requires low probe concentration. The properties of the probe allowed for direct high-resolution measurement of cortical extravascular (tissue) pO(2), opening many possibilities for functional metabolic brain studies.
Stroke; a Journal of Cerebral Circulation. May, 2011 | Pubmed ID: 21415401
Normobaric oxygen (NBO) therapy may be neuroprotective in acute ischemic stroke. However, how NBO may affect intracerebral hemorrhage is unclear. We tested NBO in a rat model of striatal intracerebral hemorrhage.