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Articles by Eric A. Dunford in JoVE
JoVE General
DNA Stable-Isotope Probing (DNA-SIP)
Department of Biology, University of Waterloo
DNA stable-isotope probing is a cultivation-independent method to identify and characterize active communities of microorganisms that are capable of utilizing specific substrates. Assimilation of substrate enriched in heavy isotope leads to incorporation of labelled atoms into microbial biomass. Density gradient ultracentrifugation retrieves labelled DNA for downstream molecular analyses.
