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In JoVE (3)
- Passaging HuES Human Embryonic Stem Cell-lines with Trypsin
- Freezing Human ES Cells
- Human ES cells: Starting Culture from Frozen Cells
Other Publications (1)
Articles by Erin Trish in JoVE
Passaging HuES Human Embryonic Stem Cell-lines with Trypsin
Erin Trish, John Dimos, Kevin Eggan
Department of Molecular and Cell Biology, Harvard
In this video we demonstrate how our lab routinely passages HuES human embryonic stem cell lines with trypsin.
Freezing Human ES Cells
Erin Trish, John Dimos, Kevin Eggan
Department of Molecular and Cell Biology, Harvard
Here we demonstrate how our lab freezes HuES human embryonic stem cell lines.
Human ES cells: Starting Culture from Frozen Cells
Erin Trish, John Dimos, Kevin Eggan
Department of Molecular and Cell Biology, Harvard
Here we demonstrate how our lab begins a HuES human embryonic stem cell line culture from a frozen stock.
Other articles by Erin Trish on PubMed
Adjusting for Risk Selection in State Health Insurance Exchanges Will Be Critically Important and Feasible, but Not Easy
Health Affairs (Project Hope). Feb, 2012 | Pubmed ID: 22323160
The Affordable Care Act calls for the establishment of state-level health insurance exchanges. The viability and success of these exchanges will require effective risk-adjustment strategies to compensate for differences in enrollees' health status across health plans. This article describes why the Affordable Care Act could lead to favorable or adverse risk selection across plans. It reviews provisions in the act and recent proposed regulations intended to mitigate the problem of risk selection. We performed a simulation that showed that under the premium rating restrictions in the law, large incentives for insurers to attract healthier enrollees will be likely to persist-resulting in substantial overpayment to plans with very healthy enrollees and underpayment to plans with very sick members. We conclude that risk adjustment based on patients' diagnoses, such as will be in place from 2014 on, will yield payments to insurers that will be more accurate than what will come solely from the age-adjusted and other rating allowed by the act. We also describe additional challenges of implementing risk adjustment.
