Articles by Eva M. Gjerde in JoVE

Cannulation of the Mouse Submandibular Salivary Gland via the Wharton's Duct

JoVE 3074 5/14/2011

Yusuke Kuriki¹, Younan Liu¹, Dengsheng Xia¹, Eva M. Gjerde¹, Saeed Khalili¹, Brennan Mui¹, Changyu Zheng², Simon D. Tran¹

¹Faculty of Dentistry, McGill University, ²National Institutes of Health, Bethesda, MD, USA

A protocol for the cannulation of the mouse submandibular salivary gland via the Wharton's duct is described. For this experiment, the trypan blue solution is used as a dyer to demonstrate how this technique effectively delivers infusions into the targeted gland, and to suggest the reliability of this new approach as a potential clinical drug/cell therapy for the regeneration of salivary glands.