Articles by Fabian Moine in JoVE

Imaging Pheromone Sensing in a Mouse Vomeronasal Acute Tissue Slice Preparation

JoVE 3311 12/06/2011

Julien Brechbühl¹, Gaëlle Luyet¹, Fabian Moine¹, Ivan Rodriguez², Marie-Christine Broillet¹

¹Department of Pharmacology and Toxicology, University of Lausanne, ²Department of Genetics and Evolution, University of Geneva

In mice, the ability to detect pheromones is principally mediated by the vomeronasal organ (VNO). Here, an acute tissue slice preparation of VNO for performing calcium imaging is described. This physiological approach allows observations of subpopulations and/or individual neurons in a living tissue and is convenient for receptor-ligand identification.